Time-resolved microwell cell-pairing array reveals multiple T cell activation profiles

Abstract

The differences in behaviour between individual cells in a large population are often important, yet are masked in bulk analyses where only average parameters are measured. One unresolved question in...

Single-Cell Microarray Chip with Inverse-Tapered Wells to Maintain High Ratio of Cell Trapping

“…The trapping method using gravitational force is called Single-Cell Microarray (SCM) [ 16 , 26 ]. As SCM is a passive method in which cells are trapped using gravitational force by seeding the cell suspension, it has been applied in a variety of ways as a minimally invasive and simple trapping method [ 27 , 28 ].…”

Microfluidic-based platforms for cell-to-cell communication studies

“…In active cell-pairing methods, external forces such as electric [21,22], magnetic [23], optical [24,25], and acoustic [26] are applied to manipulate cells for intercellular communication studies. Passive cell-pairing methods utilize physical barriers to trap single cells, such as droplet-based encapsulation [27][28][29], a microwell-based method [20,30], and structural traps with hydrodynamic-based technology [31][32][33][34][35].…”

Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip

“…In contrast to conventional multiwell plates, the confined space available in microwells facilitates localization, monitoring, and tracking of the cells. This is particularly useful for efficient high-throughput screening and time-lapse imaging at the single-cell level ( Desalvo et al., 2020 ; Zhou et al., 2020 ). In addition, manual handling of the chip or rapid microscopy stage movements during screening have little or no influence on the position of cells inside the microscale wells, where the liquid remains stationary.…”

“…In addition, manual handling of the chip or rapid microscopy stage movements during screening have little or no influence on the position of cells inside the microscale wells, where the liquid remains stationary. However, many microwell devices have limitations in terms of long-term (>24 h), live cell assays ( Desalvo et al., 2020 ; Kim et al., 2019 ; Zhou et al., 2020 ); simultaneous testing of multiple or multiplexed liquid conditions ( Desalvo et al., 2020 ; Fang et al., 2019 ; Guldevall et al., 2016 ; Kim et al., 2019 ; Olofsson et al., 2018 ; Varadarajan et al., 2011 ; Yang et al., 2017 ; Zhou et al., 2020 ); optical quality of the chip ( Chao et al., 2020 ; Desalvo et al., 2020 ; Fang et al., 2019 ; Varadarajan et al., 2011 ; Yang et al., 2017 ; Zhou et al., 2020 ); and possibility for both 2D and 3D cell cultures ( Chao et al., 2020 ; Desalvo et al., 2020 ; Fang et al., 2019 ; Guldevall et al., 2016 ; Kim et al., 2019 ; Varadarajan et al., 2011 ; Zhou et al., 2020 ).…”