Time-response changes in the thrombogenicity of platelets spread on a collagen-coated surface

Leytin, Valery; Ljubimova, E.V.; Sviridov, Dmitri; Repin, V. S.; Смирнов, В. Н.

doi:10.1016/0049-3848(80)90236-4

Cited by 18 publications

(2 citation statements)

References 7 publications

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“…[12][13][14][15] With this in mind, we have investigated a variety of platelet behaviors as an indicator of hemocompatibility for some materials and thin films commonly found in MEMS devices (silicon, silicon dioxide, silicon nitride, low-stress silicon nitride, SU-8 photoresist, and parylene). For this investigation we measured platelet adhesion, spreading, and circularity using fluorescence microscopy and computerized image analysis.…”

mentioning

confidence: 99%

Hemocompatibility of materials used in microelectromechanical systems: Platelet adhesion and morphology in vitro

Weisenberg

Mooradian

2002

J. Biomed. Mater. Res.

126

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Microelectromechanical systems (MEMS) create an opportunity for the development of smaller, cheaper, and more precise biomedical instrumentation and devices. Little is known, however, about the hemocompatibility of the materials used to fabricate these devices. Because of the potentially harmful consequences of thrombus formation, a better understanding of blood interactions with bioMEMS materials is desirable. This study is an in vitro assessment of the hemocompatibility of silicon (Si), silicon dioxide (SiO2), silicon nitride (Si3N4), low-stress silicon nitride (Si(1.0)N(1.1)), SU-8 photoresist, and parylene thin films. A polycarbonate-based polyurethane, was used as a reference material. Experiments were carried out to detect differences in platelet adhesion or morphology after contact with these materials under static conditions. Platelet adhesion on Si, Si3N4, Si(1.0)N(1.1,) and SU-8 photoresist was significantly greater (P < 0.05) than platelet adhesion on polyurethane. Adhesion on parylene and SiO(2) was not significantly different from on polyurethane (P < 0.05). The median platelet area and circularity were higher on polyurethane than all other materials. Materials that showed higher levels of platelet adhesion tended to have platelets that showed less spreading, except for SiO2, where platelets exhibited relatively low adhesion and spreading. This data suggests that Si, Si3N4, Si(1.0)N(1.1), and SU-8 photoresist may be more reactive to platelets and therefore more thrombogenic than parylene, SiO2, and polyurethane. These results may be helpful in guiding the selection of materials for use in the development of blood-contacting microelectromechanical systems.

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mentioning

confidence: 99%

Hemocompatibility of materials used in microelectromechanical systems: Platelet adhesion and morphology in vitro

Weisenberg

Mooradian

2002

J. Biomed. Mater. Res.

126

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“…surface Platelet adhesion was carried out in 16.4-mm wells of culture plates (Falcon) coated with fibrillar calf skin collagen [9]. Gel-filtered platelets (2-4 x 107) in 200~1 Tyrode solution, with or without trapidil, were introduced into each well with subsequent addition of AA, U46619 or thrombin [3,10].…”

Section: Platelet Adhesion To the Collagen-coatedmentioning

confidence: 99%

Decrease of platelet aggregation and spreading via inhibition of the cAMP phosphodiesterase by trapidil

Мазуров¹,

Menshikov²,

Leytin³

et al. 1984

FEBS Letters

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Trapidil (N,N‐diethyl‐5‐methyl[l,2,4]triazolo[l,5‐α]pyrimidine‐7‐amine) inhibits platelet spreading and aggregation induced by arachidonic acid (AA), a stable analogue of prostaglandin (PG) endoperoxides (U46619), ADP, and low concentrations of thrombin, but not by A23187 and high concentrations of thrombin. Trapidil does not affect platelet adenylate cyclase but inhibits the cAMP PDE by approx. 50%. PDE inhibition proceeds via a competitive mechanism (K i = 0.52 mM) and is not mediated by calmodulin inhibition. Trapidil does not change the platelet basal cAMP level but potentiates an increase of cAMP induced by the stable prostacyclin analogue (6β‐PGIi). These results suggest that trapidil antiplatelet effects may be due to the inhibition of platelet PDE.

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Platelet Spreading and the Formation of Thrombi-Like Aggregates on a Collagen Substrate. Effect of Soluble Inducers of Platelet Activity Under Different Hydrodynamic Conditions

Мазуров¹,

Leytin²,

Репин³

et al. 1986

Thrombosis and Thrombolysis

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Time-response changes in the thrombogenicity of platelets spread on a collagen-coated surface

Cited by 18 publications

References 7 publications

Hemocompatibility of materials used in microelectromechanical systems: Platelet adhesion and morphology in vitro

Hemocompatibility of materials used in microelectromechanical systems: Platelet adhesion and morphology in vitro

Decrease of platelet aggregation and spreading via inhibition of the cAMP phosphodiesterase by trapidil

Platelet Spreading and the Formation of Thrombi-Like Aggregates on a Collagen Substrate. Effect of Soluble Inducers of Platelet Activity Under Different Hydrodynamic Conditions

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