Time Sequence of Action of Estrogen on Nucleic Acid and Protein Synthesis in the Uterus and Blastocyst During Delayed Implantation in the Rat

Dass, C. M. S.; Mohla, Suresh; Prasad, M. R. N.

doi:10.1210/endo-85-3-528

“…Perme¬ ability and synthetic changes are established within 5 h of priming with oestradiol (Table 3). The incorporation of uridine into embryonic RNA, detected in utero by autoradiography, increases within 1 h of administering oestradiol to 'delayed implanting' rats and is maintained for up to 18 h (Dass, Mohla & Prasad, 1969). The increase in metabolic activity observed in vitro occurs without the 4 h lag period observed for 'delayed' embryos (Table 3).…”

Section: Discussionmentioning

confidence: 97%

Uterine proteins and the activation of embryos from mice during delayed implantation

Pratt¹

1977

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Summary. Ovariectomy-induced delay of implantation was used to study the role of the uterine environment in controlling implantation in mice. Labelling studies in vivo showed that uterine protein synthesis and secretion is maximal 2\p=n-\5h and 24\p=n-\30h after the oestradiol injection which initiates implantation. Embryos removed from uteri 5,12 or 30 h after oestradiol injection were able to transport and utilize precursors of nucleic acids and proteins in short-term cultures at the same rate as normal embryos, although 'delayed' embryos had low levels of activity. These results suggest that 'delayed' embryos are metabolically activated within 5 h of release from delay, perhaps because of the hormonally-induced changes in uterine proteins which occur at this time.

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“…

In earlier studies which showed that oestrogen activates the synthesis of RNA, DNA and protein in the blastocyst and uterus during delayed implantation, it was postulated that activation of the delayed blastocyst might be due to the direct action of oestrogen (Mohla, 1968;Prasad, Dass & Mohla, 1968;Dass, Mohla & Prasad, 1969).One of the earliest effects of oestrogen on the rat uterus is the release of adenosine 3\m='\5\m='\-monophosphate(cyclic ApMP), the levels of which are enhanced within 15 sec after the intravenous administration of oestrogen (Szego & Davis, 1967). Griffin & Szego (1968) showed that cyclic ApMP, like oestrogen, enhanced within 5 min the incorporation of labelled amino acids into the protein of uterine segments of untreated ovariectomized rats.

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mentioning

confidence: 99%

“…mated females were bilaterally ovariectomized on Day 3 of pregnancy (the first day of finding spermatozoa in the vagina was designated Day 1 of pregnancy) and were given 4 mg/day of progesterone in olive oil till the termination of the experi¬ ment. Precursors were instilled into the uterine lumen according to methods described in our earlier studies (Prasad et al, 1968;Dass et al, 1969). The following experiments were performed on Day 9 of delayed implantation.…”

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confidence: 99%

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STIMULATION OF RNA SYNTHESIS IN THE BLASTOCYST AND UTERUS OF THE RAT BY ADENOSINE 3'5'-MONOPHOSPHATE (CYCLIC ApMP)

Mohla¹,

Prasad²

1970

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In earlier studies which showed that oestrogen activates the synthesis of RNA, DNA and protein in the blastocyst and uterus during delayed implantation, it was postulated that activation of the delayed blastocyst might be due to the direct action of oestrogen (Mohla, 1968;Prasad, Dass & Mohla, 1968;Dass, Mohla & Prasad, 1969).One of the earliest effects of oestrogen on the rat uterus is the release of adenosine 3\m='\5\m='\-monophosphate(cyclic ApMP), the levels of which are enhanced within 15 sec after the intravenous administration of oestrogen (Szego & Davis, 1967). Griffin & Szego (1968) showed that cyclic ApMP, like oestrogen, enhanced within 5 min the incorporation of labelled amino acids into the protein of uterine segments of untreated ovariectomized rats. They postulated that the cyclic ApMP released as a result of oestrogen treatment also participates in the early effects of oestrogen on the uterus. A further effect, noted within 5 min of treatment with oestrogen, is an increase in the incorporation of [3H]uridine into RNA in the blastocyst and uterus (Mohla & Prasad, 1970). It was, therefore, of interest to study the effect of cyclic ApMP on the incorpora¬ tion of [3H]uridine into RNA in the blastocyst and uterus of the rat during delayed implantation.Colony-bred, adult, virgin, female, albino rats of the Holtzman strain (240 to 280 g) were maintained in light-controlled (14 hr light/10 hr dark), airconditioned rooms (25 ± 1°C ) and fed a standard diet. Delayed implantation was induced by the method of Cochrane & Meyer (1957), i.e. mated females were bilaterally ovariectomized on Day 3 of pregnancy (the first day of finding spermatozoa in the vagina was designated Day 1 of pregnancy) and were given 4 mg/day of progesterone in olive oil till the termination of the experi¬ ment. Precursors were instilled into the uterine lumen according to methods described in our earlier studies (Prasad et al., 1968;Dass et al., 1969). The following experiments were performed on Day 9 of delayed implantation. N6-2-0 dibutyryl adenosine 3'5' monophosphate (cyclic ApMP, Cal. Biochem., Lot No. 920036) was dissolved in sterile physiological saline (pH 7-40). The experimental group (eight rats) received an intraluminal injection of 0-05 ml saline (pH 7-40) containing 1-25 mM cyclic ApMP and 1 /tc of [3H]uridine (Sp.Act. 6-0 Ci/mM; Schwartz Bioresearch)/uterine horn. The control animals (ten rats) received an intraluminal injection of 1 µ of [3H]uridine in 0-05 ml 327

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“…The role of oestrogen is less clear. In embryos exhibiting delayed implantation, oestrogen has been shown to activate metabolism by increasing incorporation of precursors into RN A (Prasad, Dass & Mohla, 1968;Dass, Mohla & Prasad, 1969) and protein (Weitlauf & Greenwald, 1968;Prasad et al, 1968;Dass et al, 1969) and by increasing the production of C02 (Weitlauf, 1974;Torbit & Weitlauf, 1974) and glycogen (Ozias & Weitlauf, 1971). In the present experiment oestrogen caused no change in the metabolism of the preimplantation embryo in utero ; its only effect was to reverse the effects of progesterone on embryonic metabolism.…”

Section: Discussionmentioning

confidence: 41%

Effect of parenteral administration of oestrogen and progesterone on the glycogen metabolism of mouse morulae--early blastocysts in vivo

Edirisinghe¹,

Wales²

1984

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Summary. Morulae\p=n-\earlyblastocysts were pulse-labelled with radioactive glucose and subsequently transferred for 24 h to ovariectomized recipients maintained on different hormone regimens. Embryos transferred to recipients treated with progesterone utilized more glycogen than did those incubated in untreated controls. Treatment with oestrogen alone had no significant effect on glycogen turnover of transferred embryos and, when given in combination with progesterone, antagonized the effect of progesterone. Priming doses of oestrogen given >4 days before progesterone had no significant effect on the response to progesterone.The results indicate that the high levels of progesterone circulating in the mother before implantation cause glycogen degradation in the embryo and account for the low glycogen content of uterine embryos compared to those cultured in vitro in the presence of glucose.

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Time Sequence of Action of Estrogen on Nucleic Acid and Protein Synthesis in the Uterus and Blastocyst During Delayed Implantation in the Rat

Cited by 28 publications

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Uterine proteins and the activation of embryos from mice during delayed implantation

Uterine proteins and the activation of embryos from mice during delayed implantation

STIMULATION OF RNA SYNTHESIS IN THE BLASTOCYST AND UTERUS OF THE RAT BY ADENOSINE 3'5'-MONOPHOSPHATE (CYCLIC ApMP)

Effect of parenteral administration of oestrogen and progesterone on the glycogen metabolism of mouse morulae--early blastocysts in vivo

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