Time-Series Analyses of Transcriptomes and Proteomes Reveal Molecular Networks Underlying Oil Accumulation in Canola

Abstract

Understanding the regulation of lipid metabolism is vital for genetic engineering of canola (Brassica napus L.) to increase oil yield or modify oil composition. We conducted time-series analyses of transcriptomes and proteomes to uncover the molecular networks associated with oil accumulation and dynamic changes in these networks in canola. The expression levels of genes and proteins were measured at 2, 4, 6, and 8 weeks after pollination (WAP). Our results show that the biosynthesis of fatty acids is a dominant cellular process from 2 to 6 WAP, while the degradation mainly happens after 6 WAP. We found that genes in almost every node of fatty acid synthesis pathway were significantly up-regulated during oil accumulation. Moreover, significant expression changes of two genes, acetyl-CoA carboxylase and acyl-ACP desaturase, were detected on both transcriptomic and proteomic levels. We confirmed the temporal expression patterns revealed by the transcriptomic analyses using quantitative real-time PCR experiments. The gene set association analysis show that the biosynthesis of fatty acids and unsaturated fatty acids are the most significant biological processes from 2-4 WAP and 4-6 WAP, respectively, which is consistent with the results of time-series analyses. These results not only provide insight into the mechanisms underlying lipid metabolism, but also reveal novel candidate genes that are worth further investigation for their values in the genetic engineering of canola.

Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes

“…(c) and (d) Seed oil content of BC 1 F 2 (180886-180889) plants and mutant progenies from (M 3 9 Express-617) 9 (M 3 9 Express-617) crossing generations (180876 and 180877). One-way ANOVA test was performed at P < 0.05, and grouping was done using the Tukey test at P < 0.05. consumes seed reserves including the storage lipids (Wan et al, 2017). Our study provides the first successful example where knockout of BnSFAR4 and BnSFAR5 lipase genes in an oil crop resulted in higher SOC while germination rates and seed vigour remained unaffected.…”

De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism

“…In a previous study, genes related to fatty acid biosynthesis were grouped into two categories and detected in the early or later stages of seed development in tree peony [49]. Similarly, the coordinated regulation of multiple genes has been shown to promote the high accumulation of longchain unsaturated fatty acids in the seeds of tea oil camellia [50], and consistent results have been obtained in Brassica napus [51,52], Glycine max [53], and Linum usitatissimum [54]. Above all, the present results indicated that 40 days after fertilization could be a key point at which the first few steps of fatty acid biosynthesis are promoted in Z. armatum, and the subsequent reactions could be catalysed and completed in the stage occurring 62 days after fertilization, especially for long-chain fatty acid biosynthesis.…”

An integrated omics analysis reveals molecular mechanisms that are associated with differences in seed oil content between Glycine max and Brassica napus

“…To cluster and visualize the expressional patterns of all 2048 rapeseed and soybean genes in the 230 OGs, we exploited STEM software [ 56 ] to analyze the expression data of four seed development stages in rapeseed (GSE77637, [ 57 ]) and soybean (GSE42871, [ 58 ]). In this study, R3, R4, R7 and R8 stages in soybean and 2, 4, 6, and 8 weeks after pollination (WAP) in rapeseed were defined as t1’, t2’, t3’ and t4’ in soybean and t1, t2, t3 and t4 in rapeseed, respectively.…”