Time-series Analysis of Correlation between Climatic Parameters and Dengue Fever in Indonesia

Nirwantono, Rudi; Trinugroho, Joko Pebrianto; Sudigyo, Digdo; Hidayat, Alam Ahmad; Pardamean, Bens

doi:10.1109/icimcis56303.2022.10017843

Cited by 1 publication

(1 citation statement)

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“…Furthermore, for the large-scale production, it is necessary to apply certain physical settings, such as high-temperature conditions [9]. This can speed up the enzymatic process and prevent bacterial or fungal contamination [10]. Hence, using L-AI enzyme that has a high specificity and withstands a high temperature could tackle the issues.…”

Section: Introductionmentioning

confidence: 99%

Developing a DNA Marker Approach for the Sustainable Production of D-Tagatose

Trinugroho,

Hidayat,

Nirwantono

et al. 2023

E3S Web Conf.

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D-tagatose is known as a type of sugar that has low-calorie and numerous benefits. The sugar is also known to have potential for the food industry. D-tagatose can be produced biologically using the L- arabinose isomerase (L-AI) enzyme. However, sustainable production of D-Tagatose still faces an issue due to the specificity of the enzyme and the requirement of a high temperature for large-scale production. This study aims to develop an approach to discovering new bacteria that have the L-AI enzyme by implementing the DNA marker technique. We collected protein sequences from a public biological database and performed a multiple-sequence alignment. Then, the degenerate primers were designed based on the aligned sequence. The primer characterization was carried out using Oligo Calc. In-silico PCR amplification was also performed to test the primers’ specificity. Overall, the primers’ properties have met the criteria for optimally working primers. In addition, gel electrophoresis confirmed the successful amplification of the L- AI enzyme from several bacteria. Our study could be used to discover the L-AI enzyme that has the desired characteristics, which allows the sustainable production of D-tagatose.

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Section: Introductionmentioning

confidence: 99%