A formula based on the measured relation between solar radiation and photosynthetic rate was developed for computation of the annual productivity of benthic microflora. The saturating light level of 1260 pE m -' s i obtained with intact sediment cores was > 5 X higher than reported in other studies. This, together with the invalid assumption that measurement periods were representative of average conditions, accounted for discrepancies among estimates when 3 formulas employed in other studies were applied to our data. The new formula developed in this study is likely to be the most reliable estimator of annual productivity, since it is based on measured relations between photosynthesis and irradiance and does not assume that measurement periods were representative of average conditions. Its drawback is that it requires laborious analysis. In addition to the annual study, intensive sampling was conducted during a single month (60 samples incubated every other day) to obtain an estimate closely approximating actual monthly production. By using this close-to-direct measurement of monthly production, we were able to assess quantitatively the sources of error likely to be introduced by converting the hourly rates to monthly production using two of the formulas. This error was compared to the error likely to be introduced by spatial heterogeneity and by temporal heterogeneity (i.e. different sampling rates). Results indicate that the error introduced by inadequate sampling in space and time outweighs the error introduced by the commonly used conversions from measured midday productivity to estimated monthly production. Compositing many small samples from a study area into each incubation chamber efficiently addressed the problem of spatial heterogeneity. Monthly production estimated from 4 sampling days per month agreed closely with monthly production determined from 16 sampling days (mean deviation 5 YO). Estimates based on 1 or 2 sampling days m o ' were not in close agreement with the 16 sampling day estimate (mean deviation 30 YO).