Time-series responses of swine plasma metabolites to ingestion of diets containing<i>myo-</i>inositol or phytase

Cowieson, A. J.; Roos, Franz F.; Ruckebusch, J.-P.; Wilson, Jonathan; Guggenbuhl, P.; Lu, Hang; Ajuwon, Kolapo M.; Adeola, O.

doi:10.1017/s0007114517003026

Cited by 22 publications

(12 citation statements)

References 27 publications

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“…In addition, recent studies have reported a dose-dependent increase in phytate disappearance and myo-inositol concentrations in intestinal digesta of piglets with phytase [14,15,55,56]. Free myo-inositol is absorbed and can be detected in both portal and peripheral blood of pigs [15,52,56,57].…”

Section: Discussionmentioning

confidence: 99%

Application of Creep Feed and Phytase Super-Dosing as Tools to Support Digestive Adaption and Feed Efficiency in Piglets at Weaning

Lee

Febery²,

Wilcock

et al. 2021

Animals

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A total of 64 piglets were used in a 35-day study to evaluate whether creep feeding piglets on the sow or super-dosing phytase to piglets post-weaning can be used as a tool to reduce stress and support adaption to weaning. Treatments consisted of creep or no creep feed being offered pre-weaning and with or without phytase supplementation at 2000 FTU/kg post-weaning. Blood samples were collected from eight piglets per treatment on days 0 (weaning), 7 and 21 post-weaning to determine plasma cortisol and myo-inositol concentrations. Four piglets per treatment (n = 16) were administered Heidelberg pH capsules 1 week prior to weaning, on the day of weaning, as well as 7 days and 21 days post-weaning, with readings monitored over a 3 h period. In the first week post-weaning, creep-fed piglets had higher daily gains (0.23 vs. 0.14 kg/d, p < 0.05) and a lower feed conversion ratio (FCR, 0.99 vs. 1.35, p < 0.01), compared to non-creep-fed pigs. At 21 days post-weaning, irrespective of creep feed, phytase supplementation reduced FCR (1.10 vs. 1.18, p = 0.05) of piglets. Average real-time stomach pH was lower in creep-fed piglets at 1 week prior to weaning (pH 3.2 vs. 4.6, p < 0.001) and on day of weaning (pH 3.1 vs. 3.7, p < 0.01). Following weaning, phytase reduced average stomach pH of piglets at days 7 (pH 2.6 vs. 3.3, p < 0.001) and 21 (pH 2.2 vs. 2.6, p < 0.01). Both cortisol and myo-inositol concentrations in plasma decreased with age; however, cortisol levels were unaffected by either treatment. Plasma myo-inositol concentrations were higher in creep-fed piglets at day of weaning (p < 0.05) and with phytase super-dosing on day 21 (p < 0.001). These findings demonstrate that both creep feeding and phytase super-dosing are useful practices to encourage better adaption to weaning and support piglet performance. This response was not related to reduced stress in piglets, as determined by cortisol levels, but instead appears to relate to improved gastric conditions for digestion, phytate degradation and myo-inositol provision in piglets.

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Section: Discussionmentioning

confidence: 99%

Application of Creep Feed and Phytase Super-Dosing as Tools to Support Digestive Adaption and Feed Efficiency in Piglets at Weaning

Lee

Febery²,

Wilcock

et al. 2021

Animals

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“…According to Cowieson et al [17], diets that do not provide sufficient macro- and micro-nutrients (due to formulation or high levels of enzyme matrix) can prevent the pathways stimulated by myo-inositol from improving FCR by increasing protein accumulation and downregulating gluconeogenesis. Cowieson et al [15] and Schmeisser et al [32] indicated a positive effect of phytase on the concentration of myo-inositol, whose action mimics insulin, with similar effects on GLUT 4 when blood glucose concentration increases, which is involved in aspects related to gluconeogenesis and improves digestibility, weight gain, and feed conversion.…”

Section: Discussionmentioning

confidence: 99%

“…Gonçalves et al [13], however, observed that the effects in finishing pigs are variable and appear to be more evident if the concentrations of digestible phosphorus and other nutrients are marginal in the diet. The benefits of this inclusion pattern are related not only to animal performance, but also to health, well-being, and, possibly, to meat quality [14], the latter being determined by the significant increase in the plasma levels of myo-inositol [15]. Besides that, the levels of inorganic phosphorus in animal diets can be decreased by using phytase, thus reducing phosphorus excretion to the environment [16].…”

Section: Introductionmentioning

confidence: 99%

Increasing doses of phytase from Citrobacter braakii in diets with reduced inorganic phosphorus and calcium improve growth performance and lean meat of growing and finishing pigs

et al. 2019

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The objective of this study was to evaluate the effect of increasing doses of bacterial phytase (RONOZYME HiPhos) on performance and carcass characteristics of growing and finishing pigs. The study included 120 castrated males with initial weight of 23.21 ± 1.91 kg and 68 days of age, distributed in a randomized block design with five treatments and eight replicates with three animals each. The pigs were fed five corn-soybean meal-based diets: positive control (PC), supplemented with inorganic phosphorus and calcium; negative control (NC), with 0.13% reduction in available phosphorus and 0.11% in calcium; and three NC diets supplemented with 1,000, 2,000, and 3,000 phytase units (FYT)/kg in the feed. Compared with the NC diets without phytase, diets with 1,000, 2,000, and 3,000 FYT/kg inclusion increased the daily weight gain by +12% (quadratic, p<0.05) during the growing I period; +2.9, +2.9, and +10.5% (linear, p<0.01), respectively, during the growing II period; and +4.1, +5.1, and +8.2% (linear, p<0.001), respectively, over the entire experimental period. The daily feed intake increased by 0, +2.8, and +4.3% (linear, p<0.05), respectively, considering the entire experimental period; and the final live weight increased by +3.2, +4.2, and +6.1% (linear, p<0.001), respectively. The phytase treatments did not influence feed conversion ratio, carcass weight and yield, backfat thickness, loin depth and carcass lean meat. According to the European Carcass Classification (SEUROP), however, the animals fed the PC diet and the three phytase levels had more carcasses classified as E (between 55–60% lean meat) when compared to carcasses of pigs fed the NC. Supplementing increasing levels of phytase to a corn- and soybean meal-based diet with inorganic P and Ca reduction improved daily weight gain and feed intake of growing pigs, and such effects were maintained until slaughter age.

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“…Others (Guggenbuhl et al 2016;Laird et al 2018) have also found increased plasma inositol concentrations with increased dietary phytase application. A study done by Cowieson et al (2017) reported an immediate increase of plasma myo-inositol concentration after oral ingestion of myo-inositol, and 3000 FTU phytase /kg feed significantly increased plasma myo-inositol 6 h after feed intake. Therefore, the increased myo-inositol concentration in Downloaded from https://academic.oup.com/jas/advance-article-abstract/doi/10.1093/jas/skz234/5530987 by University of East Anglia user on 17 July 2019 A c c e p t e d M a n u s c r i p t the fed status at 2000 FTU/kg was likely due to the released myo-inositol by phytase.…”

Section: Inositol Phosphatesmentioning

confidence: 95%

Effect of phytase on intestinal phytate breakdown, plasma inositol concentrations, and glucose transporter type 4 abundance in muscle membranes of weanling pigs1

Kühn

Bedford

et al. 2019

Journal of Animal Science

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The objective of this present study was to determine the effects of phytase dosing on growth performance, mineral digestibility, phytate breakdown, and the level of glucose transporter type 4 (GLUT4) in muscle plasma membranes of weanling pigs. A total of 160 barrows were used in a randomized completely block design and assigned to 4 treatments for a 7-wk study. Depending on the feeding phase, diets differed in dietary calcium (Ca) and phosphorus (P) levels (positive control [PC]: 8 to 6.8g/kg Ca; 7.3 to 6.3 g/kg P; negative control [NC]: 5.5 to 5.2 g/kg Ca; 5.4 to 4.7 g/kg P). NC diets were supplemented with phytase at 0 (NC); 500 (NC + 500 FTU); or 2,000 FTU/kg (NC + 2,000 FTU) phytase units/kg. Blood was collected after fasting (day 48) or feeding (day 49) for measurement of plasma inositol concentrations. On day 49, 2 pigs per pen were euthanized, and duodenal and ileal digesta samples were collected to determine inositol phosphates (InsP6-2) concentrations. High phytase supplementation increased BW on days 21, 35, and 49 (P < 0.05). Over the entire feeding period, ADG, ADFI, and feed efficiency were increased by NC + 2,000 FTU compared with the other treatments (P < 0.05). Postprandial plasma inositol concentration was increased in NC + 2,000 (P < 0.01), but there was only a tendency (P = 0.06) of a higher fasting plasma inositol concentration in this group. Inositol concentrations in the portal vein plasma (day 49) were not different among treatments. Duodenal digesta InsP5 and InsP6 concentrations were similar in PC and NC, but higher in these 2 treatments (P < 0.05) than those supplemented with phytase. Phytase supplementation decreased InsP6-4, resulting in increased InsP3-2 and myo-inositol concentrations. Similar effects were found in ileal contents. Compared with NC, phytase supplementation resulted in greater cumulative InsP6-2 disappearance (93.6% vs. 72.8% vs. 25.0%, for NC + 2,000 FTU, NC + 500 FTU and NC, respectively, P < 0.01) till the distal ileum. Longissimus dorsi muscle plasma membrane GLUT4 concentration was increased by NC + 2,000 FTU (P < 0.01) compared with NC. In summary, high phytase supplementation increased growth performance of nursery pigs. The higher myo-inositol release from phytate could contribute to the increased expression of GLUT4 in muscle plasma membranes. Further investigation is needed to determine whether this is associated with enhanced cellular glucose uptake and utilization.

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Time-series responses of swine plasma metabolites to ingestion of diets containingmyo-inositol or phytase

Cited by 22 publications

References 27 publications

Application of Creep Feed and Phytase Super-Dosing as Tools to Support Digestive Adaption and Feed Efficiency in Piglets at Weaning

Application of Creep Feed and Phytase Super-Dosing as Tools to Support Digestive Adaption and Feed Efficiency in Piglets at Weaning

Increasing doses of phytase from Citrobacter braakii in diets with reduced inorganic phosphorus and calcium improve growth performance and lean meat of growing and finishing pigs

Effect of phytase on intestinal phytate breakdown, plasma inositol concentrations, and glucose transporter type 4 abundance in muscle membranes of weanling pigs1

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