Time to abandon dogma: CD14 is expressed by non‐myeloid lineage cells

Jersmann, Hubertus

doi:10.1111/j.1440-1711.2005.01370.x

Cited by 109 publications

(82 citation statements)

References 99 publications

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“…Consistent with these findings, sCD14 predicts higher incident cardiovascular risk [11]. However, CD14 is not just primarily expressed on monocytes/ macrophages; cell membrane or secreted CD14 protein also occurs in cells of non-hematopoietic origin [12].…”

mentioning

confidence: 56%

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Presepsin: solving a soluble (CD14) problem in sepsis?

Ackland

Prowle

2015

Intensive Care Med

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mentioning

confidence: 56%

“…Endotoxin induces transcription of CD14 mRNA in epithelial cells from several organs, including lung, kidney, and liver, as well as endothelial, microglial and vascular smooth muscle cells [12]. sCD14 is produced either through proteolytic cleavage on activated monocytes or by the secretion of a larger 56-kDa form [13,14].…”

mentioning

confidence: 99%

Presepsin: solving a soluble (CD14) problem in sepsis?

Ackland

Prowle

2015

Intensive Care Med

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“…Thus, other unknown receptors seem to mediate intracellular Ca 2+ mobilization in the presence of rough hematopoietic origin may express CD14. 47 This molecule was discovered 15 years ago, but its precise role has never been elucidated. Most of the information available about CD14 concerns its role as a coreceptor working with TLR4 in macrophages.…”

Section: Apoptotic Death Of Cdcsmentioning

confidence: 99%

“…45,46 Cells of both hematopoietic and non immune system. 47 In neurons, the CD14/ NFAT pathway may be involved in nociceptor activation, 56 whereas, in the myocardium, it may be involved in the pathogenesis of chronic heart failure. 57 We therefore need to identify the cell types in which this pathway is active and its pathophysiologic role in these cell types, if we are to identify appropriate intervention strategies.…”

Section: Apoptotic Death Of Cdcsmentioning

confidence: 99%

The dendritic cell life cycle

Granucci¹,

Zanoni²

2009

Cell Cycle

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D endritic cells (DCs) are a specialclass of leukocytes actively involved in initiating innate and adaptive immune responses against invading pathogens. They play a fundamental role in determining both the type and efficiency of adaptive immune reactions. In particular, the efficiency of adaptive responses is strictly correlated with the survival of the DCs that have encountered the antigen. In physiological conditions, the rapid death of DCs by apoptosis after an encounter with a microbe is important to prevent both aberrant activation and autoimmunity. The mechanism leading to DC apoptosis after exposure to lipopolysaccharide (LPS) was recently elucidated, with activation of the c2 and c3 isoforms of nuclear factor of activated T cells (NFAT) playing a particularly important role in this process. In particular, the exposure of DCs to LPS induces the activation of Src-family kinases and phospholipase C (PLC)γ2, the influx of extracellular Ca 2+ and calcineurin-dependent nuclear NFAT translocation. The initiation of this pathway is independent of TLR4 engagement and depends exclusively on CD14. We also consider here the possible role of CD14 in initiating this pathway and the way in which the c2 and c3 isoforms of NFAT exert their pro-apoptotic effects.

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“…CD14 is expressed strongly on the surface of monocytes and other myeloid cells, and is expressed at lower levels on B-lymphocytes, basophils, mammary cells, placental trophoblasts, gingival fibroblasts [18] and low passage number endothelial cells [19,20]. A soluble form of CD14 is also present in serum at lg/ml levels.…”

Section: Introductionmentioning

confidence: 99%

CD14 is a ligand for the integrin α4β1

Humphries

2007

FEBS Letters

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Cell adhesion mediated by the integrin a4b1 plays a key role in many biological processes reflecting both the number and functional significance of a4b1 ligands. The lipopolysaccharide (LPS) receptor, CD14, is a GPI-linked cell surface glycoprotein with a wide range of reported functions and associations, some of which overlap with that of a4b1. This overlap led us to test the specific hypothesis that a4b1 and CD14 interact directly. Jurkat T cells (a4b1 + ) were found to adhere to a recombinant CD14-Fc protein via a4b1, whilst K562 cells (a4b1 À ) did not. However, stable reexpression of the a4-subunit conferred this ability. The adhesion of both cell types to CD14 displayed activation state-dependent binding very similar to the interaction of a4b1 with its prototypic ligand, VCAM-1. In solid-phase assays, CD14-Fc bound to affinity-purified a4b1 in a dose-dependent manner that was induced by activating anti-b1 mAbs. Finally, in related experiments, JY cells (a4b7 + ) were also found to attach to CD14-Fc in an a4-dependent manner. In summary, CD14 is a novel ligand for a4b1, exhibiting similar activation-state dependent binding characteristics as other a4b1 ligands. The biological relevance of this interaction will be the subject of further studies.

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Time to abandon dogma: CD14 is expressed by non‐myeloid lineage cells

Cited by 109 publications

References 99 publications

Presepsin: solving a soluble (CD14) problem in sepsis?

Presepsin: solving a soluble (CD14) problem in sepsis?

The dendritic cell life cycle

CD14 is a ligand for the integrin α4β1

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