Time to blood culture positivity: An independent predictor of infective endocarditis and mortality in patients with Staphylococcus aureus bacteraemia

Siméon, S.; Fournier, Damien; Gustave, Claude-Alexandre; Désage, Stéphanie; Chirouze, Catherine; Curlier, Elodie; Descottes-Genon, Cécile; Hoën, Bruno; Patry, Isabelle; Vettoretti, Lucie; Chavanet, P.; Eicher, Jean‐Christophe; Gohier-Treuvelot, Sandrine; Greusard, Marie-Christine; Neuwirth, Catherine; Péchinot, A.; Piroth, Lionel; Célard, M.; Cornu, Catherine; Delahaye, François; Hadid, Malika; Rausch, Pascale; Coma, Audrey; Galtier, Florence; Géraud, Philippe; Jean-Pierre, Hélène; Moing, Vincent Le; Sportouch, Catherine; Reynes, Jacques; Aissa, Nejla; Doco‐Lecompte, T.; Goehringer, François; Keil, Nathalie; Letranchant, L.; Malela, Hepher; May, Thierry; Selton‐Suty, Christine; Bedos, Nathalie; Lavigne, Jean‐Philippe; Lechiche, Catherine; Sotto, Albert; Duval, Xavier; Habensus, Emila Ilic; Iung, Bernard; Leport, Catherine; Longuet, P; Ruimy, Raymond; Bellissant, Éric; Donnio, Pierre-Yves; Gac, Fabienne Le; Michelet, Christian; Revest, Matthieu; Tattevin, Pierre; Thébault, Elise; Alla, François; Braquet, P.; Erpelding, Marie-Line; Minary, Lætitia; Tubiana, Sarah; Bes, Michèle; Etienne, Jérôme; Lelekov-Boissard, Taïssia; Tristan, Anne; Vandenesch, François; Belkum, Alex van; Rivadeneira, Fernando; vanWamel, W.J.B.; Barbas, Sandrine; Delonca, Christine; Sussmuth, Virginie; Verchère, Anne

doi:10.1016/j.cmi.2018.07.015

Cited by 53 publications

(44 citation statements)

References 34 publications

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“…How to use TTP to predict patient outcome is less clear and requires further study [7e11]. TTP has recently been applied to predict infective endocarditis and other endovascular infections [7,12], and to predict outcome and severity of bloodstream infection [7,9,12]. TTP is an independent predictor of death (see ref.…”

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confidence: 99%

“…[7e11]), but interestingly, the relationship between TTP and mortality is not always linear (e.g. in Staphylococcus aureus bacteraemia [7,10]), and long TTP may require caution. We should probably focus on extreme values, not only on short TTP [7,10,11].…”

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confidence: 99%

“…in Staphylococcus aureus bacteraemia [7,10]), and long TTP may require caution. We should probably focus on extreme values, not only on short TTP [7,10,11]. The distributions of TTP frequently overlap in the groups of interest, and the predictive value at the patient level is all the more reduced when the populations overlap.…”

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confidence: 99%

“…Regarding TTP, S. aureus bacteraemia constitutes a difficult case for several reasons. First, TTP associated with S. aureus infective endocarditis surprisingly included both short and long TTP [7], which led the authors to assume that TTP could reflect two pathophysiological models for infective endocarditis (rapidly progressive infective endocarditis with high bacterial load; less virulent bacteria better controlled by the host). Confirmatory studies are required in this field.…”

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confidence: 99%

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Blood culture time-to-positivity: making use of the hidden information

Lamy

2019

Clinical Microbiology and Infection

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Blood culture time-to-positivity: making use of the hidden information

Lamy

2019

Clinical Microbiology and Infection

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“…The conventional culture methods for the detection of Staphylococcus in blood are time-consuming and laborious. In fact, the mean time to blood culture positivity of S. aureus is reported to be between 12 to 16 hours [68][69][70] and after this, the phenotypic tests must be implemented to identify the specific pathogen which takes about 12 to 36 hours 17 . The assay described herein took less than 2 hours to perform after a pre-enrichment step, and therefore provides a rapid, highly sensitive and reliable methodology for the detection of pathogens in blood, outstanding the standard method of BSI diagnosis.…”

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confidence: 99%

A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood

Costa

Dias

Melo

et al. 2020

Sci Rep

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Bloodstream infections (BSIs) are considered a major cause of death worldwide. Staphylococcus spp. are one of the most BSIs prevalent bacteria, classified as high priority due to the increasing multidrug resistant strains. Thus, a fast, specific and sensitive method for detection of these pathogens is of extreme importance. In this study, we have designed a novel assay for detection of Staphylococcus in blood culture samples, which combines the advantages of a phage endolysin cell wall binding domain (CBD) as a specific probe with the accuracy and high-throughput of flow cytometry techniques. In order to select the biorecognition molecule, three different truncations of the C-terminus of Staphylococcus phage endolysin E-LM12, namely the amidase (AMI), SH3 and amidase+SH3 (AMI_SH3) were cloned fused with a green fluorescent protein. From these, a higher binding efficiency to Staphylococcus cells was observed for AMI_SH3, indicating that the amidase domain possibly contributes to a more efficient binding of the SH3 domain. The novel phage endolysin-based flow cytometry assay provided highly reliable and specific detection of 1-5 CFU of Staphylococcus in 10 mL of spiked blood, after 16 hours of enrichment culture. Overall, the method developed herein presents advantages over the standard BSIs diagnostic methods, potentially contributing to an early and effective treatment of BSIs. Bloodstream infections (BSIs) are severe diseases caused by the presence of microorganisms, mainly bacteria, in blood and are characterized by high morbidity and mortality 1,2. The BSIs and associated organ dysfunctions (sepsis or septic shock) remain a life-threating disease due, partially, to the inability to rapidly detect and identify the responsible pathogens 3,4. Staphylococcus spp. are Gram-positive facultative anaerobic bacteria that frequently colonize the human body 5,6. These pathogens are becoming increasingly resistant to antibiotics and are well-established in both community and healthcare environments, being commonly isolated in intensive care units (ICU) 6,7. Staphylococcus aureus is a common cause of a variety of infections, from superficial skin infections to life-threatening diseases, including necrotizing pneumonia 8 , infective endocarditis 9 and BSIs 10. Coagulase-negative staphylococci (CoNS) have also been described as harmful to humans, causing several infections, particularly in patients with implanted medical devices 6. The empirical antibiotic therapy remains the standard of BSIs treatments 11 and its correct use within the first hour after the recognition of the BSI is recommended by the Surviving Sepsis Campaign Guidelines 11 and was reported as having a great impact on the patient survival rate 12. Nevertheless, the extensive use of broad-spectrum antibiotics and the large number of patients having negative blood culture samples and thus receiving unnecessary antibiotic treatment, are important contributors to the increase of antimicrobial resistance 13-15. Thus, sensitive, rapid, cost-efficient and specific ...

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CT-guided discitis-osteomyelitis biopsies with negative microbiology: how many days should we wait before repeating the biopsy?

et al. 2019

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Time to blood culture positivity: An independent predictor of infective endocarditis and mortality in patients with Staphylococcus aureus bacteraemia

Abstract: TTP provides reliable information in patients with S. aureus bacteraemia, on the risk of IE, and prognosis, with short TTP being an independent predictor of death. These data, readily available at no cost, may be used to identify patients who require specific attention.

Cited by 53 publications

References 34 publications

Blood culture time-to-positivity: making use of the hidden information

Blood culture time-to-positivity: making use of the hidden information

A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood

CT-guided discitis-osteomyelitis biopsies with negative microbiology: how many days should we wait before repeating the biopsy?

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