Nitrogen (N) is vital for life (Wetzel, 2001), but its excess is a pollutant that contributes to eutrophication and dead zones in rivers, estuaries, and coastal seas worldwide, with significant economic consequences (Galloway et al., 2008). In Europe, the average N surplus reached 60 kg/ha•yr in agricultural catchments (Leip et al., 2011), which resulted in generally high N levels in rivers (Green et al., 2004), with little improvement despite implementation of EU Water Framework Directive/Nitrate Directive in recent years (Bouraoui & Grizzetti, 2011). Therefore, there remains a need to understand how excess N is transported to river systems, and how it is transformed along dominant flow pathways. However, the underlying hydrological and biogeochemical processes are generally characterized by marked spatiotemporal heterogeneity, with numerous local factors interacting (e.g., climate, topography, soils, and land management practices) (Musolff et al., 2015). As a result of the integrated effect of these numerous processes, which usually have high spatial complexity, the accurate prediction for hydrological and N fluxes is still a challenging task at the catchment scale (Grizzetti et al., 2015).Spatially distributed modeling is one way to improve predictions of catchment hydrological functioning and nutrient transport processes, as spatial details can be incorporated via regional parameterization (Rozemeijer et al., 2016). More distributed models have been developed and applied over the recent decades, due to the increase in available environmental data and computational resources (Wellen et al., 2015). However, while benefiting from better spatial representation, increased model complexity poses challenges from the increase in parameter numbers, depending on the grid resolution and number of state predictions (Tang et al., 2007). The high-dimensional, nonlinear parameter spaces make it extremely difficult to identify parameter values due to the resulting equifinality (Tonkin & Doherty, 2005), sometimes leading to an intractable and uncertain calibration