Timing of administration of transforming growth factor-beta and epidermal growth factor influences the effect on material properties of the in situ frozen-thawed anterior cruciate ligament

Azuma, Hirotaka; Yasuda, Kazunori; Tohyama, Harukazu; Sakai, Toshihiko; Majima, Tokifumi; Aoki, Yoshimitsu; Minami, Akio

doi:10.1016/s0021-9290(02)00426-8

Cited by 37 publications

(38 citation statements)

References 24 publications

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“…This effect of time is consistent with previous work in this same model (Sakai et al, 2003). On the other hand, in the hemarthrosis group, many cells with a round or ovoid nucleus were scattered in the midsubstance at each period.…”

Section: Discussionsupporting

confidence: 92%

“…These results are supported by Jackson's study using a goat model (Jackson et al, 1991). Therefore, the in situ frozen-thawed rabbit ACL model has been accepted as a useful model for biomechanical studies (Azuma et al, 2003;Ju et al, 2006;Nagumo et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an application of blood clot promotes tissue repair (Anitua et al, 2004;Bab and Einhorn, 1994). A series of the authors' studies (Azuma et al, 2003;Sakai et al, 2002;Nagumo et al, 2005) reported that intra-articular administration of TGF-beta induces the extrinsic cell infiltration into the in situ frozen ACL and prevents the deterioration of its mechanical…”

Section: Discussionmentioning

confidence: 99%

“…To determine the mechanical properties of the ligament substance, we used only the anteromedial bundle of the ACL for tensile testing (Woo et al, 1992;Sakai et 9 al., 2003;Azuma et al, 2003;Nagumo et al, 2005;Ju et al, 2006), because pilot tests showed that the whole ACL substance was stronger than the bone at the insertion sites.…”

Section: Mechanical Properties Of the Anteromedial Bundle Of The Aclmentioning

confidence: 99%

“…Recently, the in situ frozen-thawed ACL, which are anatomical but acellular, have been used as an idealized ACL reconstruction model for many biomechanical studies (Jackson et al, 1991;Katsuragi et al, 2000), and has been used to determine the effect of a local application of various growth factors (Azuma et al, 2003;Ju et al, 2006;Nagumo et al, 2005;Sakai et al, 2002). In this model, the joint cavity is filled with a small amount of blood clot from peripheral vessels, but bleeding from bone marrow does not occur at all, because no bone tunnels are created.…”

Section: Introductionmentioning

confidence: 99%

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Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament

Yasuda

Sakai

Kondo

et al. 2007

Clinical Biomechanics

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Introduction. The purpose of this study is to biomechanically and histologically evaluate the effect of bleeding from bone marrow on remodeling of the in situ frozen-thawed anterior cruciate ligament.Methods. Forty-four rabbits were used. Eight rabbits were used to evaluate the amount of bleeding at days 1-4. Thirty-six rabbits were divided into 2 groups, after the right anterior cruciate ligament underwent the freeze-thaw treatment. In Group I, no treatments were applied. In Group II, a tunnel was drilled into the bone marrow the femoral intercondylar notch. Each rabbit was sacrificed at 6 or 12 weeks. We examined the mechanical properties, and the histology of the anterior cruciate ligament. Results. A small amount of blood clot was observed only on the first day inGroup I, while a large amount of blood clot was seen around the anterior cruciate ligament for 3 days after surgery in Group II. In the midsubstance, a number of cells were scattered in Group II, no cells were seen in Group I at 6 weeks. The tangent modulus showed some tendency of difference between Groups I and II at each period.Discussion. Bleeding from the bone marrow obviously enhanced extrinsic cell infiltration into the in situ frozen-thawed anterior cruciate ligament at 6 weeks, and 2 showed some effects on its mechanical properties. This study implied that, in anterior cruciate ligament reconstruction, the blood from a bone tunnel play an important role in graft remodeling.3

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Mechanical Properties Of the Anteromedial Bundle Of The Aclmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament

Yasuda

Sakai

Kondo

et al. 2007

Clinical Biomechanics

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Effect of extraction protocols and epidermal growth factor on the cellular repopulation of decellularized anterior cruciate ligament allografts

Harrison

Gratzer

2005

J Biomedical Materials Res

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We are developing a decellularized bone-anterior cruciate ligament (ACL)-bone allograft for treatment of ACL disruption in young or active patients. This study demonstrates the feasibility of seeding decellularized ACL tissue with primary ligament fibroblasts. Porcine ACLs were decellularized by one of three protocols, each differing only by the detergent/solvent used during the second wash (SDS, Triton-X, or TnBP). Porcine ACL fibroblasts were obtained by explant and seeded onto tissue samples of decellularized ACL. Culture conditions were varied to compare the relative effect of three different decellularization protocols on cellular repopulation. Culture condition variables included (1) the number of cells used for seeding, (2) the addition of epidermal growth factor (EGF), and (3) culture duration. Cellular ingrowth was assessed by metabolic activity (MTT assay), DNA quantification (Hoescht dye), and histology (H&E staining). Cell counting on histological sections demonstrated that Triton-X-and TnBP-treated ligaments were more receptive to cellular ingrowth than SDS-treated samples. The addition of EGF to culture medium did not significantly increase cellular ingrowth. Both the Triton-X and TnBP decellularization treatments provide suitable, naturally derived scaffolds for the ingrowth of primary ACL fibroblasts, and should be further investigated in the development of an allograft-derived bone-ACL-bone graft.

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Growth Factors: Application in Orthopaedic Surgery and Trauma

Balletbó

Cugat

2011

Sports Injuries

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Timing of administration of transforming growth factor-beta and epidermal growth factor influences the effect on material properties of the in situ frozen-thawed anterior cruciate ligament

Cited by 37 publications

References 24 publications

Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament

Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament

Effect of extraction protocols and epidermal growth factor on the cellular repopulation of decellularized anterior cruciate ligament allografts

Growth Factors: Application in Orthopaedic Surgery and Trauma

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