Timing of nuclear maturation and cumulus dissociation in human oocytes stimulated with clomiphene citrate, human menopausal gonadotropin, and human chorionic gonadotropin

Bomsel-Helmreich, O.; Huyen, Lan Vu N.; Durand-Gasselin, Ingrid; Salat-Baroux, J.; Antoine, Jean-Marie

doi:10.1016/s0015-0282(16)59469-2

Cited by 30 publications

(8 citation statements)

References 14 publications

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“…Earlier histological studies of mammalian follicles provide evidence that the cumulus oophorus in rats and in rabbits responds acutely to an ovulatory stimulus by rapidly expanding several fold in volume within the follicular antrum prior to ovulation (Parr, 1974;Zamboni, 1974). Massive expansion of the cumulus oophorus occurs in the intact human COC between 8 and 38 hr after an ovulatory stimulus (Bomsel-Helmreich et al, 1987) and in an earlier study, it was demonstrated that cumulus expansion in the stimulated primed immature rat occurs between 2.5 and 5 hr after the ovulatory stimulus (Larsen et al, 1986). Similar effects of an ovulatory stimulus have been observed to occur in mouse COCs in vivo in the present study.…”

Section: Observations In Other Laboratoriesmentioning

confidence: 99%

“…Normal expansion of the cumulus oophorus may influence a variety of fundamental developmental changes in both the oocyte and the spermatozoan required for normal fertilization and development of the zygote. Several studies have provided indirect evidence that cumulus expansion may be functionally related to the nuclear or cytoplasmic maturation of the oocyte (Gilula et al, 1978;Liebfried and First, 1979; Dekel et al, , 1988; Dekel and Beers, 1980;Fukui and Sakuma, 1980;Eppig, 1982;Chan et al, 1982;Liebfried and Bavister, 1983; Salustri and Siracusa, 1983; Ball et al, 1985;Larsen et al, 1986Larsen et al, , 1987; Wang and Greenwald, 1987;Bomsel-Helmreich et al, 1987;Suss et al, 1988;Murdoch, 1988; Wert and Larsen, 1989). The expansion of the cumulus oophorus may also be relevant to the success of in vitro fertilization (IVF) of cultured oocytes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronic acid synthesis and gap junction endocytosis are necessary for normal expansion of the cumulus mass

Wert

Hendrix

et al. 1990

Molecular Reproduction Devel

140

102

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The application of a quantitative videographic technique has provided an opportunity to compare the quantitative volumetric expansion of cultured oocyte complexes (COCs) to quantitative changes in gap junction down-regulation and hyaluronic acid synthesis and to investigate the effects of physiological agents that influence these processes. Results of these experiments support the idea that the down-regulation of cumulus gap junctions is required for the initial phase of cumulus cell disaggregation and confirm earlier reports that hyaluronic acid synthesis plays a major role in additional expansion of the cumulus. These studies also provide evidence that the degree of expansion observed in culture lacking substrates of hyaluronic synthesis is significantly attentuated when compared with expansion occurring in vivo and that the failure of cultured complexes to expand maximally can be overcome by the addition of substrates of hyaluronic acid synthesis to the culture medium.

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Section: Observations In Other Laboratoriesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid synthesis and gap junction endocytosis are necessary for normal expansion of the cumulus mass

Wert

Hendrix

et al. 1990

Molecular Reproduction Devel

140

102

View full text Add to dashboard Cite

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“…Human oocytes that were obtained from healthy women undergoing ovarian hyperstimulation at various times after hCG, underwent GV breakdown between 15 and 20 h post hCG and had entered metaphase I by 20 to 26 h post hCG (21). A separate study in the rhesus macaque found the majority of oocytes collected 12 hours post hCG injection to be at the GV stage while 36 hours post hCG injection yielded a majority of MII oocytes (22).…”

Section: Discussionmentioning

confidence: 99%

Nuclear maturation and structural components of nonhuman primate cumulus–oocyte complexes during in vivo and in vitro maturation

Prada

Hill

Chaffin

et al. 2009

Fertility and Sterility

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Study Objective To compare cumulus cell structure and timing of oocyte maturation of in vitro and in vivo matured nonhuman primate oocytes. Design In vivo (VVM) and in vitro (IVM) maturation of oocytes. Setting Animal cell culture laboratory. Animal(s) 48 female rhesus macaques. Interventions 15 females were administered FSH and aspirated oocytes were cultured in vitro for 0, 3, 6, 12 or 24 hours (IVM). 33 females were administered FSH and hCG and oocytes were collected 3, 6, 12, or 28–30 hours following hCG (VVM). Main Outcome Measures Nuclear maturation and microtubule scores of oocytes and actin and tubulin transzonal processes of cumulus cells. Embryo development was observed for VVM oocytes. Results The rate of nuclear maturation was faster for IVM oocytes compared to VVM oocytes. Actin transzonal processes decreased 0 to 12 hours post hCG for VVM oocytes. Tubulin transzonal processes of IVM and VVM oocytes decreased from 0 to 24 hours and 0 to 3 hours respectively. Embryo development improved as VVM time increased. Conclusions Nuclear maturation and remodeling of cumulus oocyte complex structural components associated with IVM do not parallel that of oocyte maturation in vivo, indicating that in vitro culture conditions continue to be sub-optimal.

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“…The authors concluded that follicular flushing recovered oocytes of optimal reproductive competence. Mechanisms proposed for this finding included oocytes recovered by aspiration alone representing an early hCG reaction and being over-matured [27]. Oocytes achieved secondarily by flushing might have benefited from longer contact with cumulus cells [28].…”

Section: Quality Of Oocytes Obtained From Follicular Flushingmentioning

confidence: 99%

Is there a benefit in follicular flushing in assisted reproductive technology?

Hill

Levens

2010

Current Opinion in Obstetrics &Amp; Gynecology

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Randomized controlled trials consistently demonstrate no benefit and increased procedural time with follicular flushing in both normal and poor-responding ART patients. Nonrandomized data suggest a possible role for follicular flushing in natural cycle or minimal stimulation ART and in those undergoing in-vitro maturation IVF cycles; however, randomized controlled trials are needed to verify this finding. Presently, there is insufficient evidence to recommend the routine use of follicular flushing.

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Timing of nuclear maturation and cumulus dissociation in human oocytes stimulated with clomiphene citrate, human menopausal gonadotropin, and human chorionic gonadotropin

Cited by 30 publications

References 14 publications

Hyaluronic acid synthesis and gap junction endocytosis are necessary for normal expansion of the cumulus mass

Hyaluronic acid synthesis and gap junction endocytosis are necessary for normal expansion of the cumulus mass

Nuclear maturation and structural components of nonhuman primate cumulus–oocyte complexes during in vivo and in vitro maturation

Is there a benefit in follicular flushing in assisted reproductive technology?

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