2003
DOI: 10.1091/mbc.e02-11-0715
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TINA Interacts with the NIMA Kinase inAspergillus nidulansand Negatively Regulates Astral Microtubules during Metaphase Arrest

Abstract: The tinA gene of Aspergillus nidulans encodes a protein that interacts with the NIMA mitotic protein kinase in a cell cycle-specific manner. Highly similar proteins are encoded in Neurospora crassa and Aspergillus fumigatus. TINA and NIMA preferentially interact in interphase and larger forms of TINA are generated during mitosis. Localization studies indicate that TINA is specifically localized to the spindle pole bodies only during mitosis in a microtubule-dependent manner. Deletion of tinA alone is not letha… Show more

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Cited by 30 publications
(30 citation statements)
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References 55 publications
(77 reference statements)
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“…First, MTs generated from the SPB could interact with cortical proteins such as ApsA (Num1 in S. cerevisiae) and MT-plus-end localized dynein could be subsequently activated at the cortex and pull the MTs with the attached nucleus. Second, MTs produced from adjacent nuclei overlap in the cytoplasm and sliding of the antiparallel filaments leads to the translocation of nuclei (Fischer, 1999;Osmani et al, 2003) (Fig. 8).…”
Section: Mt-dependent Nuclear Movementmentioning
confidence: 99%
“…First, MTs generated from the SPB could interact with cortical proteins such as ApsA (Num1 in S. cerevisiae) and MT-plus-end localized dynein could be subsequently activated at the cortex and pull the MTs with the attached nucleus. Second, MTs produced from adjacent nuclei overlap in the cytoplasm and sliding of the antiparallel filaments leads to the translocation of nuclei (Fischer, 1999;Osmani et al, 2003) (Fig. 8).…”
Section: Mt-dependent Nuclear Movementmentioning
confidence: 99%
“…tinC and A. nidulans hetC were deleted as described previously (31). Briefly, an 8,802-kb genomic fragment containing tinC was cloned into the Litmus 29 plasmid vector (New England Biolabs) as an AflII/AvrII restriction fragment.…”
Section: Race Pcrmentioning
confidence: 99%
“…For deletion of A. nidulans hetC, an A. nidulans hetC-containing bacterial artificial chromosome clone (20C15) was identified from an A. nidulans genomic library as described previously (31). This bacterial artificial chromosome was used to generate a deletion construct in which A. nidulans hetC was replaced by a Zeo r /pyroA ϩ cassette.…”
Section: Race Pcrmentioning
confidence: 99%
“…Although several studies have documented the behavior of nuclei in conidia and hyphae, none have yet provided a detailed description of mitosis in living cells of N. crassa. This is in stark contrast to studies of mitosis performed with other filamentous fungi, including Aspergillus nidulans (43,44), Nectria haematococca (1,2,69), and Ashbya gossypii (26). Green fluorescent protein (GFP) labeling has greatly facilitated the observation of nuclei in living filamentous fungi (23,24,26,66,70) and has been particularly useful for time-lapse studies (22,44).…”
mentioning
confidence: 99%