TiO₂ nanotube immobilised 5-lipoxygenase-mediated screening and isolation of anti-inflammatory active compounds from the leaves of lonicera japonica thunb

Abstract

In this work, a highly effective separation approach mediated by 5-Lipoxygenase (5-LOX) was established for screening and isolation of anti-inflammatory ingredients from leaves of Lonicera japonica Thunb. (LLJT). Using 5-LOX immobilised on TiO 2 nanotubes as a microreactor, the targeted screening was exploited by combining with HPLC-MS system. Four compounds confirmed as luteolin, luteoside, lonicerin, and isochlorogenic acid C and a fraction (M1) were screened out to be potent inhibitors of 5-LOX. Their anti-inflammatory activities were further investigated and confirmed by RAW 264.7 cells inflammation model and rat foot swelling model. Furthermore, M1 was prepared by MCI GEL CHP20P column chromatography, and further separated by Pre-HPLC. One new compound confirmed to be 5,7,3′,4′-tetrahydroxyflavone-7-O-sambubioside was first isolated from LLJT. The results provide a new method for the effective separation of active components derived from natural products. Highlights A 5-LOX mediated separation method was established for isolation of anti-inflammatory compounds. An anti-inflammatory ingredient was separated by MCI GEL CHP20P column chromatography. One new compound was first isolated from leaves of Lonicera japonica Thunb. 5-LOX was immobilised on TiO 2 nanotubes and exploited by combining with HPLC-MS system. The anti-inflammatory activity of screened components was evaluated.

Lonicerin alleviates ovalbumin-induced asthma of mice <i>via</i> inhibiting enhancer of zeste homolog 2/nuclear factor-kappa B signaling pathway

“…Activation of Th2 cells drives the inflammatory response and its downstream factors coordinate allergic inflammatory cascade, including IgE synthesis and eosinophil survival and maturation [ 24 ]. Previous studies demonstrated that lonicerin had anti-inflammatory effect in vivo and in vitro , and for example, it reduced the levels of pro-inflammatory markers in the rat with ulcerative colitis and mice with acute lung inflammation and injury [ 15 , 19 , 25 ]. Our results revealed that lonicerin inhibited Th2 activation, which was evidenced by the decreased OVA-specific IgE, thereby reducing the inflammatory phenotype, including decreased mucus distribution and inflammatory index, reduced cell count, and lower inflammatory factors.…”

Lonicera japonica Thunb extract ameliorates lipopolysaccharide-induced acute lung injury associated with luteolin-mediated suppression of NF-κB signaling pathway

“…In clinical practice, various Chinese patent medicine injections including LJT and the extracts are widely utilized for treating respiration-related disorders, which have made favorable curative effect. Louicera japonica Thunb extract (LTE) is rich in various chemical components, mainly for organic acids, including, Lut, chlorogenic acid, isochlorogenic acid, caffeic acid and palmitic acid [ 12 ]. Among them, Lut is a typical kind of natural flavonoid compound and an effective active monomer with strong oxidative capacity [ 13 ].…”