TiO₂ nanotubes synthesised on Ti-6Al-4V ELI exhibits enhanced osteogenic activity: A potential next-generation material to be used as medical implants

Dual response of osteoblast activity and antibacterial properties of polarized strontium substituted hydroxyapatite—Barium strontium titanate composites with controlled strontium substitution

“…MG63 human osteoblast‐like cells, derived from human osteosarcoma, were used for cell culture studies. Cells were cultured on the unpolarized and polarized 5SrHA‐5BST and 10SrHA‐10BST composites, as per our previous report 25 …”

“…The MG63 cell adhesion and proliferation was also estimated on unpolarized and polarized 5SrHA‐5BST and 10SrHA‐10BST composites by using CCK 8 assay following the protocols as performed in our previous study 25 …”

Fabrication of silver doped nano hydroxyapatite-carrageenan hydrogels for articular cartilage applications

“…Table 1 depicts the primers used in RT-PCR analyses. ImageJ 1.41 software was used to analyse the bands obtained from the electrophoresis of a 1% (w/V) agarose gel [21].…”

“…The culture media was renewed every alternate day. After achieving confluence, the non-viable cells were removed by a trypsin-EDTA solution (0.5 g/L trypsin and 0.2 g/L EDTA, Gibco) and the cells were shifted to a new tissue culture flask [21].…”

“…The specimens were added with 10 µL of CCK-8 solution and incubated for 3 h. The optical density (OD) was then quantified with the help of a microplate reader at 490 nm. For each incubation time of 1, 7 and 14 days, the absorbance values were taken as the mean of three measurements [21].…”