TiO2-based photocatalyst Generated Reactive Oxygen Species cause cell membrane disruption of Staphylococcus aureus and Escherichia coli O157:H7

Yong, So-Seum; Lee, Jae Ik; Kang, Dong‐Hyun

doi:10.1016/j.fm.2022.104119

Cited by 20 publications

(11 citation statements)

References 63 publications

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“…However, the red fluorescence emitted by g‐C 3 N 4 nanosheets was found to be negligible. The bactericidal mechanism was confirmed to involve the generation of ROS through photocatalysis, which subsequently accumulated intracellularly and caused damage to the cell membrane, ultimately leading to cell death 55 . The photocatalytic activity of g‐C 3 N 4 @ZnO is superior to that of g‐C 3 N 4 , resulting in the generation of more ROS.…”

Section: Resultsmentioning

confidence: 98%

“…The photocatalytic effects generated ROS, which subsequently overwhelmed the antioxidant defense systems in pathogens, leading to cell death and membrane damage. 55 The cell envelope, composed of the polymer peptidoglycan, encases gram-negative bacterial cells. This robust structure determines bacterial cell morphology and confers resistance to osmotic lysis.…”

Section: Bacterial Cytomembrane Integrity Measurementmentioning

confidence: 99%

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Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Cai,

Huang,

Feng

et al. 2023

Pest Management Science

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The utilization of nonmetallic inorganic nanomaterials for antimicrobial photocatalytic technology has emerged as a promising approach to combat drug‐resistant bacteria. Recently, g‐C3N4 nanosheets have attracted significant attention due to their exceptional stability, degradability, low cost, and remarkable antibacterial properties. In this study, a facile electrostatic self‐assembly approach was utilized to functionalize ZnO nanoparticles with g‐C3N4 nanosheets, resulting in the formation of g‐C3N4@ZnO nanoparticle composites. The Z‐shaped heterojunction architecture of these composites facilitates efficient separation of photogenerated electron‐hole pairs and enhances visible light catalytic performance. Moreover, the formation of the g‐C3N4@ZnO heterostructure showed a higher photocatalytic capacity and the generation of reactive oxygen species than g‐C3N4 nanosheets. The photocatalytic antibacterial mechanisms of g‐C3N4@ZnO at the transcriptomic level primarily involve disrupting bacterial membrane synthesis and inhibiting motility and energy metabolism. Therefore, the antibacterial mechanism of g‐C3N4@ZnO can be attributed to a combination of physical membrane damage, chemical damage (ROS enhancement) and inhibition of chemotaxis, biofilm formation and flagellar motility. These findings collectively provide novel high potential and insights into the practical application of photocatalysts in plant disease management.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 98%

Section: Bacterial Cytomembrane Integrity Measurementmentioning

confidence: 99%

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Cai,

Huang,

Feng

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

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“…The addition of nanofillers, such as silver, zinc oxide and titanium dioxide nanoparticles, has been widely used in science to obtain starch-based nanocomposites for external packaging due to their outstanding properties and antimicrobial characteristics [ 20 , 21 , 22 ]. Particularly, titanium dioxide nanoparticles (TiO 2 NP) have shown bactericidal capacity against E. coli and S. aureus and the ability to reflect and absorb UVA (320–400 nm) and UVB (290–320 nm) rays, protecting against sunburn, photoaging and extending product’s shelf life after harvesting [ 23 , 24 , 25 ]. In addition, FDA has a restriction of titanium dioxide of 1 wt.% when it is used as a food additive [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of TiO2 Nanoparticles and Extrusion Process on the Physicochemical Properties of Biodegradable and Active Cassava Starch Nanocomposites

et al. 2023

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Biodegradable polymers have been strongly recognized as an alternative to replace traditional petrochemical plastics, which have become a global problem due to their long persistence in the environment. In this work, the effect of the addition of titanium dioxide nanoparticles (TiO2NP) on the morphology, physicochemical properties and biodegradation under industrial composting conditions of cassava starch-based nanocomposites obtained by extrusion at different screw speeds (80 and 120 rpm) were investigated. Films performed at 120 rpm (S120 and S120-TiO2NP) showed completely processed starch and homogeneously distributed nanoparticles, leading to much more flexible nanocomposites than those obtained at 80 rpm. The incorporation of TiO2NP led to an increase in storage modulus of all films and, in the case of S120-TiO2NP, to higher strain at break values. From the Kohlrausch–Williams–Watts theoretical model (KWW), an increase in the relaxation time of the nanocomposites was observed due to a decrease in the number of polymer chains involved in the relaxation process. Additionally, S120-TiO2NP showed effective protection against UV light, greater hydrophobicity and faster biodegradation in compost, resulting in a promising material for food packaging applications.

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“…Next, the ROS react with organic molecules on the microbes’ outer surface, such as proteins (porins, proteins involved in oxidative stress response, in transport, and in bacterial metabolism) and polyunsaturated fatty acids [ 32 ], and destroy the cell wall and membrane, as evidenced by scanning electron microscopy [ 12 , 33 ]. Interestingly, it has recently been reported that different TiO 2 configurations generate different types of ROS, exerting differential bactericidal efficacies [ 34 ]. In the case of viruses, ROS attack the phospholipid bilayer and the envelope and/or capsid proteins [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Photo-Fenton and TiO2 Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization

et al. 2023

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Photocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton’s reagent and TiO2, respectively. A comprehensive study of homogeneous and heterogeneous photocatalysis on a range of microorganisms will significantly establish the most efficient method. Here, we report a comparative study of TiO2- and Fe+3-based photocatalytic inactivation under UV-A of diverse microorganisms, including Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, bacterial spores (Bacillus stearothermophilus spores) and viruses (MS2). We also present data on the optimization of TiO2 photocatalysis, including optimal catalyst concentration and H2O2 supplementation. Our results indicate that both photo-Fenton and TiO2 could be successfully applied for the management of microbial loads in liquids. Efficient microorganism inactivation is achieved with homogeneous photocatalysis (7 mg/L Fe+3, 100 mg/L H2O2, UV-A) in a shorter processing time compared to heterogeneous photocatalysis (0.5 g/L TiO2, UV-A), whereas similar or shorter processing is required when heterogenous photocatalysis is performed using microorganism-specific optimized TiO2 concentrations and H2O2 supplementation (100 mg/L); higher H2O2 concentrations further enhance the heterogenous photocatalytic inactivation efficiency. Our study provides a template protocol for the design and further application for large-scale photocatalytic approaches to inactivate pathogens in liquid biomedical waste.

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TiO2-based photocatalyst Generated Reactive Oxygen Species cause cell membrane disruption of Staphylococcus aureus and Escherichia coli O157:H7

Cited by 20 publications

References 63 publications

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Effect of TiO2 Nanoparticles and Extrusion Process on the Physicochemical Properties of Biodegradable and Active Cassava Starch Nanocomposites

Photo-Fenton and TiO2 Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization

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TiO2-based photocatalyst Generated Reactive Oxygen Species cause cell membrane disruption of Staphylococcus aureus and Escherichia coli O157:H7

Cited by 20 publications

References 63 publications

Composite g‐C3N4@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Composite g‐C3N4@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Effect of TiO2 Nanoparticles and Extrusion Process on the Physicochemical Properties of Biodegradable and Active Cassava Starch Nanocomposites

Photo-Fenton and TiO2 Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization

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Product

Resources

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Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease