TIP49b, a New RuvB-like DNA Helicase, Is Included in a Complex Together with Another RuvB-like DNA Helicase, TIP49a

Kanemaki, Masato; Kurokawa, Yumiko; Matsuura, Toru; Makino, Yasutaka; Masani, Abdull; Okazaki, Katsuichiro; Morishita, Takashi; Tamura, Tomohiro

doi:10.1074/jbc.274.32.22437

Cited by 135 publications

(136 citation statements)

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“…RUVBL2 was previously shown to be also required for the growth and survival of yeast. 7,31,32 Our data have shed some light on the mechanisms of apoptosis induced by RUVBL2 deficiency. We show that RUVBL2 down-regulation is associated with the increased conformational activation of the proapoptotic proteins Bak-1 and Bax.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, RUVBL2 has been found in supramolecular complexes that do not include ␤-catenin, 11 and ␤-catenin-independent functions of RUVBL2 have already been identified. 15 It is also noteworthy that RUVBL2 is required for the viability of yeast, 7,31,32 an organism devoid of ␤-catenin. In this context, 1 of the salient findings of this study was that in addition to its overexpression in HCC, RUVBL2 was also found in the cytoplasm of HCC cells.…”

Section: Discussionmentioning

confidence: 99%

“…14 RUVBL2 also has limited homology to the bacterial RuvB adenosine triphosphatedependent DNA helicase. Human RUVBL2 has indeed been shown to have ATPase activity, 7,11 whereas its DNA helicase activity has been disputed. 7,11 RUVBL2 is found in several nuclear high-molecular-weight chromatin-remodeling complexes.…”

mentioning

confidence: 99%

“…Human RUVBL2 has indeed been shown to have ATPase activity, 7,11 whereas its DNA helicase activity has been disputed. 7,11 RUVBL2 is found in several nuclear high-molecular-weight chromatin-remodeling complexes. 10,11 Recently, it has been found that RUVBL2 is able to interact with ␤-catenin, resulting in altered T-cell factor/lymphoid enhancer-binding factormediated transcription.…”

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confidence: 99%

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Overexpression and role of the ATPase and putative DNA helicase RuvB-like 2 in human hepatocellular carcinoma

et al. 2007

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Using a proteomic analysis of human hepatocellular carcinoma (HCC), we identified the overexpression in 4 tumors of RuvB-like 2 (RUVBL2), an ATPase and putative DNA helicase known to interact with ␤-catenin and cellular v-myc myelocytomatosis viral oncogene homolog (c-myc). RUVBL2 expression was further analyzed in tumors with quantitative reverse-transcription polymerase chain reaction analysis and immunohistochemistry; in addition, RUVBL2 expression in a HuH7 cell line was silenced by small interfering RNA or increased with a lentiviral vector. RUVBL2 messenger RNA overexpression was confirmed in 72 of 96 HCC cases, and it was associated with poorly differentiated tumors (P ‫؍‬ 0.02) and a poor prognosis (P ‫؍‬ 0.02) but not with ␤-catenin mutations or c-myc levels. Although RUVBL2 was strictly nuclear in normal hepatocytes, tumoral hepatocytes exhibited additional cytoplasmic staining. There was no mutation in the coding sequence of RUVBL2 in 10 sequenced cases. Silencing RUVBL2 in HuH7 HCC cells reduced cell growth (P < 0.001) and increased apoptosis, as shown by DNA fragmentation (P < 0.001) and caspase 3 activity (P < 0.005). This was associated with an increased expression of several proapoptotic genes and with an increased conformational activation of Bak-1 and Bax. On the other hand, HuH7 cells with an overexpression of RUVBL2 grew better in soft agar (P < 0.03), had increased resistance to C2 ceramide-induced apoptosis (P < 0.001), and gave rise to significantly larger tumors when injected into immunodeficient Rag2/␥c mice (P ‫؍‬ 0.016). Conclusion: RUVBL2 is overexpressed in a large majority of HCCs. RUVBL2 overexpression enhances tumorigenicity, and RUVBL2 is required for tumor cell viability. These results argue for a major role of RUVBL2 in liver carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Overexpression and role of the ATPase and putative DNA helicase RuvB-like 2 in human hepatocellular carcinoma

et al. 2007

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“…The R2TP module RUVBL1 and RUVBL2 (RVB1 and RVB2; TIP49 and TIP48; pontin and reptin) RUVBL1 and RUVBL2 are ATPases of the AAA+ superfamily (ATPase associated with diverse cellular activities) and are related to the prokaryotic DNA helicase RuvB (Qiu et al 1998;Kanemaki et al 1999), which is involved in homologous recombination and doublestrand break repair (see Fig. 2 for a schematic representation of the R2TP module components).…”

Section: Introductionmentioning

confidence: 99%

New insights into the biogenesis of nuclear RNA polymerases?This paper is one of a selection of papers published in this special issue entitled “Canadian Society of Biochemistry, Molecular & Cellular Biology 52nd Annual Meeting — Protein Folding: Principles and Diseases” and has undergone the Journal's usual peer review process.

Cloutier

Coulombe

2010

Biochem. Cell Biol.

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More than 30 years of research on nuclear RNA polymerases (RNAP I, II, and III) has uncovered numerous factors that regulate the activity of these enzymes during the transcription reaction. However, very little is known about the machinery that regulates the fate of RNAPs before or after transcription. In particular, the mechanisms of biogenesis of the 3 nuclear RNAPs, which comprise both common and specific subunits, remains mostly uncharacterized and the proteins involved are yet to be discovered. Using protein affinity purification coupled to mass spectrometry (AP-MS), we recently unraveled a high-density interaction network formed by nuclear RNAP subunits from the soluble fraction of human cell extracts. Validation of the dataset using a machine learning approach trained to minimize the rate of false positives and false negatives yielded a highconfidence dataset and uncovered novel interactors that regulate the RNAP II transcription machinery, including a set of proteins we named the RNAP II-associated proteins (RPAPs). One of the RPAPs, RPAP3, is part of an 11-subunit complex we termed the RPAP3/R2TP/prefoldin-like complex. Here, we review the literature on the subunits of this complex, which points to a role in nuclear RNAP biogenesis.

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Highly Enantioselective Rhodium‐Catalyzed Asymmetric 1,4‐Addition Reactions of Arylboronic Acids to Acyclic α,β‐Unsaturated Compounds: The Formal Synthesis of (−)‐Indatraline

Wei

Yeh

Kuo

et al. 2011

Chemistry A European J

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H 4 )], [1] also known as the Zeise salt, in 1827, considerable numbers of olefin-coordinated transition-metal complexes have been synthesized for extensive use in organometallic and organic chemistry.[2]They also serve as useful precatalysts in the field of asymmetric catalysis. The labile olefins are rapidly and quantitatively replaced by optically active ligands that bear heteroatoms, such as nitrogen and phosphorus, which exhibit a stronger binding affinity towards metal atoms than the olefins, efficiently enabling the in situ generation of chiral catalysts.[3] However, the utilization of optically active olefin ligands as chiral modifiers had not received much attention until the independent studies of Hayashi et al. [4] and Carreira et al. [5] Their discoveries have demonstrated that chiral dienes with the appropriate geometry can form reasonably stable metal complexes due to chelation effects. These chiral diene-metal catalysts, particularly the rhodium complexes, have since been tested in catalytic asymmetric carboncarbon bond-forming reactions and were found to exhibit higher catalytic activities and enantioselectivities than phosphine ligands.[6] Since then, the design, synthesis, and investigation of chiral dienes for a wide range of asymmetric transformations has attracted much attention among synthetic organic chemists. [7, 8]

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TIP49b, a New RuvB-like DNA Helicase, Is Included in a Complex Together with Another RuvB-like DNA Helicase, TIP49a

Cited by 135 publications

References 45 publications

Overexpression and role of the ATPase and putative DNA helicase RuvB-like 2 in human hepatocellular carcinoma

Overexpression and role of the ATPase and putative DNA helicase RuvB-like 2 in human hepatocellular carcinoma

Highly Enantioselective Rhodium‐Catalyzed Asymmetric 1,4‐Addition Reactions of Arylboronic Acids to Acyclic α,β‐Unsaturated Compounds: The Formal Synthesis of (−)‐Indatraline

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