Tirucallane-type triterpenoids from the fruits of Phellodendron chinense Schneid and their cytotoxic activities

Yan, Chen; Zhang, Yundong; Wang, Xinghui; Geng, Shun-Dong; Wang, Tian-Yuan; Sun, Mao; Liang, Wei-Zhe; Zhang, Weiqing; Zhang, Xiaodong; Luo, Heng

doi:10.1016/j.fitote.2016.07.020

Cited by 14 publications

(6 citation statements)

References 16 publications

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“…For thousands of years, the dried bark of Phellodendron amurense Rup. has been used as a TCM to remove damp heat and cure heat accumulation in the intestines and stomach, diarrhea, and other syndromes (Li et al, 2006;Yan et al, 2016). Coptis chinensis Franch is a common component of traditional Chinese herbal formulae used to relieve abdominal pain and diarrhea (Tjong et al, 2011); Fraxinus rhynchophylla Hance is commonly used to treat gout, arthritis, diarrhea, and bacillary dysentery in clinics and can remove wetness and heat (Fu et al, 2014); and Huanglian, Huangbai, and Qinpi synergistically enhance the Baitouweng effect of PD.…”

Section: Introductionmentioning

confidence: 99%

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Hua

Ma²,

Zhang

et al. 2020

Front. Pharmacol.

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Ethnopharmacological Relevance: Diarrhea is a major medical problem in clinical practice. According to the theory of traditional Chinese medicine (TCM), different types of diarrhea should be treated with different TCM formulations based on the targeted medical condition. Dampness-heat diarrhea (DHD) is a serious diarrheal disease and Pulsatilla decoction (PD), a TCM, has been found effective against DHD. Objective: The aim of this study was to clarify the mechanism of action of PD in DHD using an untargeted lipidomics strategy. Materials and Methods: Wistar rats were randomized to four groups, including the control group, model group, PD groups and self-healing group. The PD groups were given a daily intragastric gavage of PD at doses of 3.76 g/kg. The rat model of DHD established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment, drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of DHD. Then the clinical symptoms and signs, blood routine, serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed to evaluate DHD model and therapeutic effect of PD. Lipid biomarkers of DHD were selected by comparing the control and model groups with the colon lipidomics technology and an ultra-high performance liquid chromatography (UHPLC) coupled with Q Exactive plus mass analyzer. Multivariate statistical analysis and pattern recognition were employed to examine different lipids within the colon of PD-treated rats. Results: The clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of DHD. After treatment with PD, the clinical symptoms and signs of the rats with DHD were improved; the indexes of blood routine and inflammatory cytokines levels tended to be normal. The lipidomics profile of the model group were

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Section: Introductionmentioning

confidence: 99%

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Hua

Ma²,

Zhang

et al. 2020

Front. Pharmacol.

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“…The traditional medicinal herb P. chinense has been used to reduce heat and fever and to release toxins in Asian countries, including Korea, China, and Japan [ 10 ]. Here, we demonstrated that an ethanol extract of P. chinense had a potent effect on Alzheimer’s disease-related in vitro bioevents, such as AChE activation, antioxidation, and the protection against neuronal cell damage.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, P. chinense is a major composition of various herbal formulae such as Hwanglyeonhaedok-tang and Dangguiyukhwang-tang. Of interest, recent scientific reports determined the biological activities of P. chinense and its active components, which include anticancer [ 10 , 11 ], anti-inflammation [ 12 ], antidiabetes (and its complications) [ 13 ], and antialgal [ 14 ] activities, as well as neuroprotection [ 15 ]. However, Xian et al [ 15 ] described the neuroprotective effect of P. chinense using PC12 cells derived from pheochromocytoma of the rat adrenal medulla, and not neuronal cells.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Quantification and the In Vitro Acetylcholinesterase Inhibitory Activity of Phellodendron chinense and Its Components

et al. 2017

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The dried bark of Phellodendron chinense has been used as a traditional herbal medicine to remove damp heat, relieve consumptive fever, and cure dysentery and diarrhea. In the present study, we performed quantitative analyses of the two components of P. chinense, phellodendrine and berberine, using high-performance liquid chromatography. A 70% ethanol extract of P. chinense was prepared and the two components were separated on a C-18 analytical column using a gradient solvent system of acetonitrile and 0.1% (v/v) aqueous trifluoroacetic acid. The ultraviolet wavelength used for detection was 200 nm for phellodendrine and 226 nm for berberine. The analytical method established here showed high linearity (correlation coefficient, ≥0.9991). The amount of phellodendrine and berberine used was 22.255 ± 0.123 mg/g and 269.651 ± 1.257 mg/g, respectively. Moreover, we performed an in vitro acetylcholinesterase (AChE) activity assay and an amyloid-β aggregation test to examine the biological properties of phellodendrine and berberine as therapeutic drugs for Alzheimer’s disease. Phellodendrine and berberine inhibited AChE activity in a dose-dependent manner (IC50 = 36.51 and 0.44 μM, respectively). In contrast, neither phellodendrine nor berberine had an effect on amyloid-β aggregation. The P. chinense extract and phellodendrine, but not berberine, exhibited antioxidant activity by increasing radical scavenging activity. Moreover, P. chinense demonstrated a neuroprotective effect in hydrogen peroxide-treated HT22 hippocampal cells. Overall, our findings suggest that P. chinense has potential as an anti-Alzheimer’s agent via the suppression of the enzymatic activity of acetylcholinesterase and the stimulation of antioxidant activity.

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“…There are 3 compounds in PCS that have been found to resist three types of tumours which included leukemic cell lines K562 and HEL, breast cancer cell line MDA, and prostate cancer cell line PC3. The compound [(21S, 23R) epoxy-24-hydroxy-21β, 25-diethoxy] tirucalla-7-en-3-one has a relatively strong effect as Adriamycin against four tumors with the measurement of IC 50 ; toonaciliatin K and piscidinol A have a comparably moderate effect in this regard [42]. There are 9 most effective compounds of PC for prostate cancer, namely, magnoflorine-O-glucuronide, (p-hydroxybenzyl)-6, 7-dihydroxy-N-methyl tetrahydro iso-quinoline-7-O-p-D-glucopyranosid, magnoflorine, menisperine-O-glucuronide, menisperine, berberine, Jatrorrhizine, obaculactone , and obacunone [43].…”

Section: Pharmacologymentioning

confidence: 99%

Phellodendri Cortex: A Phytochemical, Pharmacological, and Pharmacokinetic Review

Sun

Lenon

Yang

2019

Evidence-Based Complementary and Alternative Medicine

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Background. Phellodendri Cortex (PC) or Huang Bai. According to the scientific database of China Plant Species and Chinese pharmacopeia 2015 edition, PC has two main species which are Phellodendron amurense Rupr (PAR) or “Guan Huang bai” in Chinese and Phellodendron chinense Schneid (PCS) or “Chuan Huang bai” in Chinese. The crude drugs of PAR and PCS are also called Phellodendri amurensis cortex (PAC) and Phellodendri chinense cortex (PCC), respectively. The medicinal part of the plant is the dried trunk bark. PC has comprehensive therapeutic effects which include anti-inflammatory, antimicrobial, anticancer, hypotensive, antiarrhythmic, antioxidant, and antipyretic agents. The exact ingredients in PC and its species are not fully summarised. Aim of the Study. This study was designed to review and evaluate the pharmacological actions of compounds and to explore the pharmacokinetic knowledge of PC and its species and to also identify the chemical compound(s) with a potential therapeutic effect on atopic dermatitis. Methods. “Huang Bai” and its English, botanical, and pharmaceutical names were used as keywords to perform database search in Encyclopaedia of traditional Chinese Medicines, PubMed, EMBASE, MEDLINE, Science Direct, Scopus, Web of Science, and China Network Knowledge Infrastructure. The data selection criteria included all the studies that were related to the phytochemical, pharmacological, and pharmacokinetic perspectives of PC and its species or their active constituents. More importantly, the voucher number has been provided to ensure the genuine bark of PC used as the medicinal part in the studies. Results. 140 compounds were summarized from PC and its species: specifically, 18 compounds from PCC, 44 compounds from PCS, 34 compounds from PAC, and 84 compounds from PAR. Obacunone and obaculactone are probably responsible for antiatopic dermatitis effect. PC and its species possess a broad spectrum of pharmacological actions including anti-inflammatory effect, antibacterial effect, antiviral effect, antitumor effect, antigout effect, antiulcer effect, neuroprotective effect, and antiatopic dermatitis effect. PC could widely distribute in plasma, liver, spleen, kidney, and brain. Berberine may be responsible for the toxic effect on the susceptible users with hemolytic disease or in the peripartum and neonatal period. Conclusions. The compounds of the crude bark of PC and its subspecies have showcased a wide range of pharmacological effects. Pharmacological efficacies of PC are supported by its diverse class of alkaloid, limonoid, phenolic acid, quinic acid, lignan, and flavonoid. Obacunone and obaculactone could be the bioactive compounds for atopic dermatitis management. PC and its subspecies are generally safe to use but extra care is required for certain conditions and group of people.

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Tirucallane-type triterpenoids from the fruits of Phellodendron chinense Schneid and their cytotoxic activities

Cited by 14 publications

References 16 publications

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Pulsatilla Decoction Can Treat the Dampness-Heat Diarrhea Rat Model by Regulating Glycerinphospholipid Metabolism Based Lipidomics Approach

Phytochemical Quantification and the In Vitro Acetylcholinesterase Inhibitory Activity of Phellodendron chinense and Its Components

Phellodendri Cortex: A Phytochemical, Pharmacological, and Pharmacokinetic Review

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