Tissue and cellular morphological changes in growth plate explants under compression

Differential limb loading in miniature pigs (Sus scrofa domesticus): a test of chondral modeling theory

“…For the current study, this suggests that elevated mechanical loading could result in lower scaled cell counts for the reserve and hypertrophic zones and higher counts for the proliferative zone, as the loading regimen increases the rate at which reserve zone cells enter the proliferative phase and hypertrophic cells become incorporated into the bony matrix of the limb element. Work by Amini et al found zonal differences in chondrocyte bulk strains, with greater strains recorded in the proliferative and hypertrophic chondrocytes, as well as a decrease of the cell to matrix volume ratio following compression in the reserve and hypertrophic zones, coupled with an increase in the proliferative zone (Amini et al, 2010). This demonstrates further support for possible alternative explanations for findings that contradict traditional chondral modeling theory.…”

The Role of Mechanobiology in the Attachment of Tendon to Bone

“…These gradients also contribute to variations in mechanical behavior that likely influence the tissue response to mechanical loading. In rat growth plates, higher compressive strains were detected in the hypertrophic region compared to the resting/proliferative region [30,31]. The resting zone is also stiffer than the combined proliferative and hypertrophic zones [32,33].…”

A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth

“…To overcome the limitation of over-segmentation of the watershed method, marker-controlled watershed techniques have been developed to improve the performance (Yang et al, 2006;Fenistein et al, 2008;Plissiti et al, 2011, Koyuncu et al, 2012Arco et al, 2015). Before applying a segmentation method, morphological analysis is helpful to extract useful information from the cellular images (Amini et al, 2010;Plissiti et al, 2011). A two-step binarization method is proposed to split the clumped nuclei (LaTorre et al, 2013).…”