Tissue and plasma free amino acid detection by LC-MS/MS method in high grade glioma patients

Toklu, Sureyya; Kemerdere, Rahşan; Kacira, Tibet; Gürses, Murat Serdar; Aksungar, Fehime Benli; Tanrıverdi, Taner

doi:10.1007/s11060-023-04329-z

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…We found high levels of serum histidine in glioblastoma patients compared to the controls. This was parallel with the result of Toklu et al, where histidine levels were elevated in patients with high-grade glioblastoma [55]. Previous studies found that low plasma levels of histidine were observed in patients with shorter overall survival in glioblastoma patients.…”

Section: Discussionsupporting

confidence: 84%

“…The higher concentration of lysine observed in our study in patients with glioma and meningioma, considering the division by gender, was also observed in patients with head and neck cancers and sarcoma [52][53][54]. Toklu et al found high levels of serum lysine in high-grade glioma patients [55]. Mören et al observed that serum lysine levels of highgrade glioma decreased during radiotherapy when the disease is indolent [56].…”

Section: Discussionsupporting

confidence: 77%

“…Cadoni et al noted a high level of aminoadipic acid in the serum of patients with a reduced risk of advanced head and neck cancer [54]. Toklu et al did not observe statistically significant differences in aminoadipic acid between high-grade glioma and the control group determined in serum and brain tissue [55].…”

Section: Discussionmentioning

confidence: 96%

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Comparative Analysis of Amino Acid Profiles in Patients with Glioblastoma and Meningioma Using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS)

Kośliński,

Pluskota,

Koba

et al. 2023

Molecules

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Brain tumors account for 1% of all cancers diagnosed de novo. Due to the specificity of the anatomical area in which they grow, they can cause significant neurological disorders and lead to poor functional status and disability. Regardless of the results of biochemical markers of intracranial neoplasms, they are currently of no diagnostic significance. The aim of the study was to use LC-ESI-MS/MS in conjunction with multivariate statistical analyses to examine changes in amino acid metabolic profiles between patients with glioblastoma, meningioma, and a group of patients treated for osteoarthritis of the spine as a control group. Comparative analysis of amino acids between patients with glioblastoma, meningioma, and the control group allowed for the identification of statistically significant differences in the amino acid profile, including both exogenous and endogenous amino acids. The amino acids that showed statistically significant differences (lysine, histidine, α-aminoadipic acid, phenylalanine) were evaluated for diagnostic usefulness based on the ROC curve. The best results were obtained for phenylalanine. Classification trees were used to build a model allowing for the correct classification of patients into the study group (patients with glioblastoma multiforme) and the control group, in which cysteine turned out to be the most important amino acid in the decision-making algorithm. Our results indicate amino acids that may prove valuable, used alone or in combination, toward improving the diagnosis of patients with glioma and meningioma. To better assess the potential utility of these markers, their performance requires further validation in a larger cohort of samples.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 77%

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Comparative Analysis of Amino Acid Profiles in Patients with Glioblastoma and Meningioma Using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS)

Kośliński,

Pluskota,

Koba

et al. 2023

Molecules

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Development of a rapid and robust hydrop interaction liquid chromatography tandem mass spectrometry method for the detection of 13 endogenous amino acids as well as trimethylamine oxide in serum and tissues of the mice

Hu,

Liu,

Yao

et al. 2024

Biomedical Chromatography

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This work aimed to establish an HILIC‐MS/MS method to simultaneously determine the levels of 13 endogenous amino acids and trimethylamine oxide in the biological samples from the mice. Electrospray ion source was used for the analysis of mass spectrometry. The 20 min separation was applied in a Dikma Inspire Hilic column (2.1 × 100.0 mm, 3 μM). Positive ion mode under an MRM model gave a satisfying response value. The limits of quantitation were evaluated by accuracy from −12.59% to 7.89% and precision from 1.77% to 14.00% as well as acceptable interday and intraday precision, matrix effect, recovery, and stability. Later, the assay was successfully used to measure the concentrations of the determinands in the biological samples. Individual and tissue distribution differences for these metabolites were observable. The amino acids had a consistent highest content in the spleens, while the lowest levels were found in the livers. Alanine was the most abundant amino acid in the serum, and taurine kept the highest content in all of the tissues. Trimethylamine oxide remained low level, especially in the liver samples.

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The Effects of Hypoxia on the Immune–Metabolic Interplay in Liver Cancer

He,

Xu,

Liu

et al. 2024

Biomolecules

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M2-like macrophages promote tumor growth and cancer immune evasion. This study used an in vitro model to investigate how hypoxia and tumor metabolism affect macrophage polarization. Liver cancer cells (HepG2 and VX2) and macrophages (THP1) were cultured under hypoxic (0.1% O2) and normoxic (21% O2) conditions with varying glucose levels (2 g/L or 4.5 g/L). Viability assays and extracellular pH (pHe) measurements were conducted over 96 hours. Macrophages were exposed to the tumor-conditioned medium (TCM) from the cancer cells, and polarization was assessed using arginase and nitrite assays. GC-MS-based metabolic profiling quantified TCM meta-bolites and correlated them with M2 polarization. The results showed that pHe in TCMs decreased more under hypoxia than normoxia (p < 0.0001), independent of glucose levels. The arginase assay showed hypoxia significantly induced the M2 polarization of macrophages (control group: p = 0.0120,0.1%VX2-TCM group: p = 0.0149, 0.1%HepG2-TCM group: p < 0.0001, 0.1%VX2-TCMHG group: p = 0.0001, and 0.1%HepG2-TCMHG group: p < 0.0001). TCMs also induced M2 polarization under normoxic conditions, but the strongest M2 polarization occurred when both tumor cells and macrophages were incubated under hypoxia with high glucose levels. Metabolomics revealed that several metabolites, particularly lactate, were correlated with hypoxia and M2 polarization. Under normoxia, elevated 2-amino-butanoic acid (2A-BA) strongly correlated with M2 polarization. These findings suggest that targeting tumor hypoxia could mitigate immune evasion in liver tumors. Lactate drives acidity in hypoxic tumors, while 2A-BA could be a therapeutic target for overcoming immunosuppression in normoxic conditions.

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Tissue and plasma free amino acid detection by LC-MS/MS method in high grade glioma patients

Cited by 3 publications

References 29 publications

Comparative Analysis of Amino Acid Profiles in Patients with Glioblastoma and Meningioma Using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS)

Comparative Analysis of Amino Acid Profiles in Patients with Glioblastoma and Meningioma Using Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS)

Development of a rapid and robust hydrop interaction liquid chromatography tandem mass spectrometry method for the detection of 13 endogenous amino acids as well as trimethylamine oxide in serum and tissues of the mice

The Effects of Hypoxia on the Immune–Metabolic Interplay in Liver Cancer

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