Tissue-Autonomous Phenylpropanoid Production Is Essential for Establishment of Root Barriers

Andersen, Tonni Grube; Molina, David J.; Kilian, Joachim; Franke, Rochus; Ragni, Laura; Geldner, Niko

doi:10.1016/j.cub.2020.11.070

Cited by 56 publications

(58 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon infection or after treatment with the suberin hormone inducer abscisic acid, suberin was chemically detected in petioles (Robb et al ., 1991), and suberin as well as lignin coatings, were both deposited in intercellular spaces between parenchyma cells adjoining a xylem vessel or infusing and occluding pit membranes coatings (Robb et al ., 1991; Street et al ., 1996). Besides, inhibition of the phenylpropanoid pathway by blocking PAL enzyme inhibited the formation of both lignin and suberin coatings (Street et al ., 1996), in agreement with the ferulic acid requirement to correctly deposit suberin (Andersen et al ., 2021) and reinforcing our observations of the presence of a ferulate/feruloylamide-derived polymer detected in H7996 R. solanacearum KOH-treated samples observed under UV light (Fig. 4).…”

Section: Discussionsupporting

confidence: 76%

“…Ferulic acid present in the suberin and lignin-like fractions is proposed to link both polymers (Graça, 2010) and its continuous production has been demonstrated essential for suberin deposition (Andersen et al ., 2021). Ferulic acid amides, such as feruloyltyramine and feruloyloctopamine, have been described as structural components of the lignin-like polymer and in the phenolic soluble fraction of suberizing wound-healing potato tuber (Negrel et al ., 1996; Razem and Bernards, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato roots

Kashyap

Capellades

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

The soil borne pathogen Ralstonia solanacearum is the causing agent of bacterial wilt, a devastating disease affecting major agricultural crops. R. solanacearum enters plants through the roots and reaches the vasculature, causing rapid wilting. We recently showed that tomato varieties resistant to bacterial wilt restrict bacterial movement in the plant. In the present work we go a step forward by identifying the physico-chemical nature of the barriers induced in resistant tomato roots in response to R. solanacearum. We describe that resistant tomato specifically responds to infection by assembling de novo a structural barrier at the vasculature formed by a ligno-suberin coating and tyramine-derived hydroxycinnamic acid amides (HCAAs). On the contrary, susceptible tomato does not form these reinforcements in response to the pathogen but instead displays lignin structural changes compatible with its degradation. Further, we show that overexpressing genes of the ligno-suberin pathway in a commercial susceptible variety of tomato restricts R. solanacearum movement inside the plant and slows disease progression, enhancing resistance to the pathogen. We thus propose that the induced barrier in resistant plants does not only restrict the movement of the pathogen, but may also prevent cell wall degradation by the pathogen and confer anti-microbial properties.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato roots

Kashyap

Capellades

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…These data demonstrate that transient expression of a single gene, either StMYB74 or StMYB102, was sufficient to activate all steps required for the synthesis, export, and deposition of suberin into a proper lamellar ultrastructure in the correct subcellular location. Given recent findings that phenylpropanoid biosynthesis and the deposition of ester-linked ferulic acid are critical for anchoring polyaliphatic suberin to the cell wall (Andersen et al, 2021), these results support a hypothesis that StMYB74 and StMYB102 are likely regulators of multiple aspects of suberin biosynthesis and deposition, including polyaliphatic and ligno-suberin.…”

Section: Identification and Characterization Of Tfs That Regulate Suberin Biosynthesissupporting

confidence: 75%

Transcriptional regulation of wound suberin deposition in potato cultivars with differential wound healing capacity

et al. 2021

View full text Add to dashboard Cite

Wounding during mechanical harvesting and post-harvest handling results in tuber desiccation and provides an entry point for pathogens resulting in substantial post-harvest crop losses. Poor wound healing is a major culprit of these losses. Wound tissue in potato (Solanum tuberosum) tubers, and all higher plants, is composed of a large proportion of suberin that is deposited in a specialized tissue called the wound periderm. However, the genetic regulatory pathway controlling wound-induced suberization remains unknown. Here, we implicate two potato transcription factors, StMYB102 (PGSC0003DMG400011250) and StMYB74 (PGSC0003DMG400022399), as regulators of wound suberin biosynthesis and deposition. Using targeted metabolomics and transcript profiling from the wound healing tissues of two commercial potato cultivars, as well as heterologous expression, we provide evidence for the molecular-genetic basis of the differential wound suberization capacities of different potato cultivars. Our results suggest that (i) the export of suberin from the cytosol to the apoplast and ligno-suberin deposition may be limiting factors for wound suberization, (ii) StMYB74 and StMYB102 are important regulators of the wound suberization process in tubers, and (iii) polymorphisms in StMYB102 may influence cultivar-specific wound suberization capacity. These results represent an important step in understanding the regulated biosynthesis and deposition of wound suberin and provide a practical foundation for targeted breeding approaches aimed at improving potato tuber storage life.

show abstract

“…Also, nine MYB transcription factors were also upregulated in April (Table 1 ), indicating the importance of this gene family in contributing to phellem cell differentiation. From them, AtMYB106 was described to regulate cuticular lipid-deposits (wax and cutin) in epidermal cells 32 and three MYBs were related with lignification and/or suberization: AtMYB36 promotes the lignification of the Casparian strip and suberization of endodermis 33 , 34 , and AtMYB3 and AtMYB4 repress phenylpropanoid biosynthesis 35 – 37 and when overexpressed lead to dysfunctional Casparian strip lignification and lower suberin deposition in endodermis and phellem 38 . Overall, these MYB transcription factors are candidates to early regulate in phellogen derivatives to phellem cells the phenylpropanoid and fatty acyl biosynthetic processes that lead to the final accumulation of soluble or insoluble phenylpropanoids (such as condensed tannins or lignin) or fatty-acyl derived compounds (such as waxes or suberin).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation

Fernández-Piñán

Boher

Soler

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem or cork. Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far. Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell. At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified. Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated. Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development. The putative function of relevant genes identified are discussed in the context of phellem ontogeny.

show abstract

Tissue-Autonomous Phenylpropanoid Production Is Essential for Establishment of Root Barriers

Cited by 56 publications

References 58 publications

Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato roots

Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato roots

Transcriptional regulation of wound suberin deposition in potato cultivars with differential wound healing capacity

Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation

Contact Info

Product

Resources

About