Tissue banking in Bangladesh: 12 years of experience (2003–2014)

Akhtar, Naznin; Rahman, Shaifur; Jamil, Hossen Mohammad; Arifuzzaman, Md.; Miah, Munsur; Asaduzzaman, Sikder M.

doi:10.1007/s10561-016-9549-6

Cited by 6 publications

(11 citation statements)

References 22 publications

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“…Human placenta/amniotic sacs were collected during cesarean sections and kept in 4 °C. Within 24 h we processed and prepared the membrane as described before [24, 38]. Amniotic membranes were separated carefully from chorionic membrane manually and washed with PBS (Gibco) repeatedly until a complete elimination of blood clots was achieved.…”

Section: Methodsmentioning

confidence: 99%

“…Clinically, amnion has been applied as a wound covering bioactive material to heal split thickness skin burn wounds as well as for children with partial-thickness facial burns [36, 37]. From our experience, amniotic membrane as a graft for burn wounds enclosure in Bangladesh appears to be advantageous [38]. But the limited number of membrane donors and the lack of trained personnel in amniotic graft processing are major challenges.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract

Rahman

Islam

Rana

et al. 2019

BMC Complement Altern Med

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Background Skin burn wound is a notable medical burden worldwide. Rapid and effective treatment of burnt skin is vital to fasten wound closure and healing properly. Amniotic graft and Aloe vera are widely used as wound managing biomaterials. Sophisticated processing, high cost, availability, and the requirement of medics for transplantation limit the application of amnion grafts. We aim to prepare a novel gel from amnion combined with the Aloe vera extract for burn wound healing which overcome the limitations of graft. Methods Two percent human amniotic membrane (AM), Aloe vera (AV) and AM+AV gels were prepared. In vitro cytotoxicity, biocompatibility, cell attachment, proliferation, wound healing scratch assays were performed in presence of the distinct gels. After skin irritation study, second-degree burns were induced on dorsal region of Wistar rats; and gels were applied to observe the healing potential in vivo. Besides, macroscopical measurement of wound contraction and re-epithelialization; gel treated skin was histologically investigated by Hematoxylin and eosin (H&E) staining. Finally, quantitative assessment of angiogenesis, inflammation, and epithelialization was done. Results The gels were tested to be non-cytotoxic to nauplii and compatible with human blood and skin cells. Media containing 500 μg/mL AM+AV gel were observed to promote HaCaT and HFF1 cells attachment and proliferation. In vitro scratch assay demonstrated that AM+AV significantly accelerated wound closure through migration of HaCaT cells. No erythema and edema were observed in skin irritation experiments confirming the applicability of the gels. AV and AM+AV groups showed significantly accelerated wound closure through re-epithelialization and wound contraction with P < 0.01. Macroscopically, AM and AM+AV treated wound recovery rates were 87 and 90% respectively with P < 0.05. Histology analysis revealed significant epitheliazation and angiogenesis in AM+AV treated rats compared to control ( P < 0.05). AM+AV treated wounds had thicker regenerated epidermis, increased number of blood vessels, and greater number of proliferating keratinocytes within the epidermis. Conclusion We demonstrated that a gel consisting of a combination of amnion and Aloe vera extract has high efficacy as a burn wound healing product. Amniotic membrane combined with the carrier Aloe vera in gel format is easy to produce and to apply.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract

Rahman

Islam

Rana

et al. 2019

BMC Complement Altern Med

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“…Legislation in many Asian countries also does not address technical specifications or the quality management of tissue banking. To overcome this problem, Bangladesh’s only tissue bank, the Tissue Banking and Biomedical Research Unit for instance, follows the IAEA, EATB and AATB guidelines [ 68 ]. In Japan, the Japanese Society of Tissue Transplantation (JSTT) consolidated more comprehensive guidelines in the Asia Pacific region and published three tissue banking related documents: (1) Guideline on the Safety, Storage, and Application of Human Tissue in Medical Practice [ 69 ]; (2) Guideline on Ethical Issues in Application of Human Tissue to Medical Practice [ 70 ]; and (3) Basic Standards for Operating A Human Tissue Bank & Quality Assessment Form [ 71 ].…”

Section: Reviewmentioning

confidence: 99%

A Review of Skin Banking Guidelines and Standards Worldwide: Towards the Harmonization of Guidelines for Skin Banking in Therapeutic Applications for the Regions under the Asia Pacific Burn Association (APBA)

et al. 2020

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Currently, there are no harmonized guidelines which govern skin banking in the Asia Pacific region. Therefore, skin banks are either unregulated or rely on their nation’s legislation or international accreditation to uphold their quality standards. A new set of skin banking guidelines was developed through a comprehensive review and collation of best international practices for the Asia Pacific Burn Association (APBA) members, from donor screening and testing, to skin recovery, processing, storage and distribution, and quality assurance. National regulatory requirements reviewed include the European directives, Australia’s Therapeutic Goods Administration and Singapore’s tissue banking standards. Further technical and quality management recommendations are referenced from the American Association of Tissue Banks (AATB), the United States Food and Drug Administration standards and guidance documents, various relevant European guides, Japanese Society of Tissue Transplantation guidelines and the Asia Pacific Association of Surgical Tissue Banking. Adapted mainly from the AATB standards, the new Asia Pacific Burn Association Guidelines for Skin Banking in Therapeutic Applications offer a comprehensive manual, addressing: governance and contracts; staff responsibilities; quality management; facilities, equipment and supplies management; donor consent and testing; and recommendations of good practices pertaining to skin recovery, processing, storage and distribution. Besides complementing current generic regulations, they provide technical specifications of major aspects unaddressed in most legislations. This inaugural set of new regional skin banking guidelines would be a start for regional members of the APBA to adopt, and will hopefully culminate in a set of standards so that, in the long run, skin allografts from this region can be of similar quality, which can simplify import process and facilitate the exchange of allografts between members.

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“…For this reason, IAEA contained a broader responsibility in the development of quality, safety, ethical issues at international level and to establish viable networks for providing sterilized tissues through the American Association of Tissue Banks (AATB) and the European Association of Tissue Banks (EATB). Previously, we have reported our experience in tissue banking service in Bangladesh in terms of the method of donor selection, processing, storage and quality assurance of the tissue allografts (Akhtar et al 2016). In this article, we presented the impact of the IAEA programme in tissue banking practices and outcomes in Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

Human bone and amniotic membrane banking in Bangladesh for grafting: the impact of the international atomic energy agency (IAEA) programme

et al. 2020

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The idea of establishing a human tissue bank in Bangladesh was started in 1985. However, in 2003, with the active cooperation of international atomic energy agency (IAEA) and Bangladesh Atomic Energy Commission, a tissue bank laboratory was upgraded as a unit for tissue banking and research. Due to increasing demand of allograft, this unit was transformed as an independent institute ''Institute of Tissue Banking and Biomaterial Research (ITBBR)'' in 2016. This is the only human tissue bank in Bangladesh, which processes human bone and amniotic membrane to provide safe and cost-effective allografts for transplantation. Importantly, banking of human cranial bone as autograft has also started at ITBBR. These processed grafts are sterilized using gamma radiation according to the IAEA Code of Practice for the radiation sterilization of tissues allografts. The amount of grafts produced by the ITBBR from 2007 to 2018 were 120,800 cc of bone chips, 45,420 cm 2 of amniotic membranes, 277 vials of de-mineralized bone granules (DMB), 95 pieces of massive bones, and 134 pieces of cranial bones. Overall, 112,748 cc of bone chips, 40,339 cm 2 of amniotic membranes, 174 vials of DMB, 44 pieces of massive bones, and 64 pieces of cranial bones were transplanted successfully. Nevertheless, to cope up with the modern advanced concepts of cell and tissue banking for therapeutic purpose, ITBBR is working to set up facilities for skin banking, stem cells banking including amniotic and cord blood derived stem cells and scaffold designing. To ensure the quality, safety, ethical and regulatory issues are sustainable in cell and tissue banking practices, ITBBR always works with the Government of Bangladesh for enhancing the national tissue transplantation programme within the contemporary facilities.

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Tissue banking in Bangladesh: 12 years of experience (2003–2014)

Cited by 6 publications

References 22 publications

Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract

Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract

A Review of Skin Banking Guidelines and Standards Worldwide: Towards the Harmonization of Guidelines for Skin Banking in Therapeutic Applications for the Regions under the Asia Pacific Burn Association (APBA)

Human bone and amniotic membrane banking in Bangladesh for grafting: the impact of the international atomic energy agency (IAEA) programme

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