Tissue bioaccumulation patterns, xenobiotic biotransformation and steroid hormone levels in Atlantic salmon (Salmo salar) fed a diet containing perfluoroactane sulfonic or perfluorooctane carboxylic acids

Mortensen, Anne S.; Letcher, Robert J.; Cangialosi, Maria Vittoria; Chu, Shi-Jye; Arukwe, Augustine

doi:10.1016/j.chemosphere.2011.01.067

Cited by 59 publications

(37 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, eicosanoids, including prostaglandins, thromboxanes and leukotrienes, belong to an extensive family of oxygenated metabolites derived from 20-carbon PUFAs such as ARA and EPA [52], which primarily act as potent local modulators in cells [53]. In accordance with previous findings [39], [49], [50] and as demonstrated in the present study, salmon liver is capable of FAD6-desaturation of ALA to stearidonic acid (18∶4n3) followed by elongation and FAD5 desaturation to EPA, in addition to FAD6 desaturation of Linoleic acid (18∶2n-6) to γ-linoleic acid (18∶3n6) followed by elongation to Dihomo-γ-linolenic acid (DGLA, 20∶3n-6) and FAD5 desaturation to ARA [54]. Our data show that hypoxic conditions reduced several n-3 PUFAs such as ALA, DHA and EPA, which were not in accordance with the increased expression of FAD5, FAD6 and FAE mRNA.…”

Section: Discussionsupporting

confidence: 87%

“…Previously, we showed that PFOSA produced time- and concentration-dependent alterations in the hepatic membrane content of several classes of FAs in salmon hepatocytes [39], [49], [50]. Herein, we show that exposure of cells to hypoxic conditions produced changes in hepatic membrane FA composition, similar to the effect of PFOSA alone, and combined exposure to hypoxia and PFOSA, further modulated the effects of hypoxic conditions alone.…”

Section: Discussionsupporting

confidence: 56%

“…Regardless, we reported recently that PFOA, PFOS and PFOSA modulated lipid homeostasis and PPAR transcription in salmon in vivo and in vitro systems [39], [50]. It has also been suggested that certain POPs can interact with transcription factors in a similar manner as FAs, acting as a PPAR agonist [82].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Modulation of Membrane Lipid Composition and Homeostasis in Salmon Hepatocytes Exposed to Hypoxia and Perfluorooctane Sulfonamide, Given Singly or in Combination

2014

Self Cite

View full text Add to dashboard Cite

The relative importance of environmental hypoxia due to global climate change on organismal ability to adapt to chemical insult and/or mechanisms of these responses is not well understood. Therefore, we have studied the effects of combined exposure to perfluorooctane sulfonamide (PFOSA) and chemically induced hypoxia on membrane lipid profile and homeostasis. Primary salmon hepatocytes were exposed to PFOSA at 0, 25 and 50 µM singly or in combination with either cobalt chloride (CoCl2: 0 and 150 µM) or deferroxamine (DFO: 0 and 100 µM) for 24 and 48 h. CoCl2 and DFO were used to induce cellular hypoxia because these two chemicals have been commonly used in animal experiments for this purpose and have been shown to increase hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF) levels. Fatty acid (FA) profiles were determined by GC-MS, while gene expression patterns were determined by quantitative PCR. Hypoxic condition was confirmed with time-related increases of HIF-1α mRNA levels in CoCl2 and DFO exposed cells. In general, significant alterations of genes involved in lipid homeostasis were predominantly observed after 48 h exposure. Gene expression analysis showed that biological responses related to peroxisome proliferation (peroxisome proliferator-activated receptors (PPARs) and acyl coenzyme A (ACOX)) and FA desaturation (Δ5- and Δ6-desaturases: FAD5 and FAD6, respectively) and elongation (FAE) were elevated slightly by single exposure (i.e. either PFOSA, CoCl2 or DFO exposure alone), and these responses were potentiated in combined exposure conditions. Principal component analysis (PCA) showed a clustering of peroxisome proliferation responses at transcript levels and FA desaturation against membrane FAs levels whose changes were explained by PFOSA and chemically induced hypoxia exposures. Overall, our data show that most of the observed responses were stronger in combined stressor exposure conditions, compared to individual stressor exposure. In general, our data show that hypoxia may, singly or in combination with PFOSA produce deleterious health, physiological and developmental consequences through the alteration of membrane lipid profile in organisms.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 56%

See 1 more Smart Citation

Modulation of Membrane Lipid Composition and Homeostasis in Salmon Hepatocytes Exposed to Hypoxia and Perfluorooctane Sulfonamide, Given Singly or in Combination

2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reproduction and the endocrine system of fish might be susceptible towards both endocrine disrupting chemicals (EDCs) (Arcand-Hoy and Benson, 1998), multiple climatic and environmental stressors (Baroiller and D'Cotta, 2001;Schreck et al, 2001). PFOS has previously been found to affect endocrine parameters, sexual development and reproduction in fish (Ankley et al, 2005;Fang et al, 2012;Mortensen et al, 2011;Oakes et al, 2005;Wang et al, 2011). However, to our knowledge there are no studies that have examined in fish how elevated pCO2 might modulate the response to PFOS exposure on hormonal and biotransformation systems.…”

Section: Introductionmentioning

confidence: 74%

“…Quantitative analysis was performed using the isotope dilution method with MPFOS as internal standard, a five-point calibration curve (0-400 ng/mL) for the analyte (PFOS) and a fixed concentration (20ng/mL) of internal standard was used. The procedure for sample work-up and HPLC-MS/MS analysis are previously described by Mortensen et al (2011) and Chu and Letcher (2009). Water CO2 saturation during the CO2 exposure period was estimated based on measured pH using the CO2calc application (Robbins et al, 2010), assuming a salinity of 33.8ppm, total alkalinity of 2223 µmol kg -1 , a temperature of 10°C and atmospheric pressure of 10 dbar.…”

Section: Chemical Analysesmentioning

confidence: 99%

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

et al. 2014

Self Cite

View full text Add to dashboard Cite

In the aquatic environments, the predicted changes in water temperature, pO2 and pCO2 could result in hypercapnic and hypoxic conditions in aquatic animals. These conditions are thought to affect several basic cellular and physiological mechanisms. Yet, possible adverse effects of elevated CO2 (hypercapnia) on teleost fish, as well as combined effects with emerging and legacy environmental contaminants are poorly investigated. In this study, juvenile Atlantic cod (Gadus morhua) were divided into groups and exposed to three different water bath PFOS exposure regimes (0 (control), 100 and 200 µg/L) for 5 days at 1hr/day, followed by three different CO2-levels (normocapnia, moderate (0.3%) and high (0.9%)). The moderate CO2 level is the predicted near future (within year 2300) level, while 0.9% represent severe hypercapnia. Tissue samples were collected at 3, 6 and 9 days after initiated CO2 exposure. Effects on the endocrine and biotransformation systems were examined by analyzing levels of sex steroid hormones (E2, T, 11-KT) and transcript expression of estrogen responsive genes (ERα, Vtg-α, Vtg-β, ZP2 and ZP3). In addition, transcripts for xenobiotic metabolizing enzymes (cyp1a and cyp3a) and hypoxiainducible factor (HIF-1α) were analyzed. Hypercapnia alone produced increased levels of sex steroid hormones (E2, T, 11-KT) with concomitant increase of estrogen responsive genes, while PFOS produced weak and time-dependent effects on E2-inducible gene transcription. Combined PFOS and hypercapnia exposure produced increased effects on sex steroid levels as observed for hypercapnia alone, with transcript expression patterns that are indicative of time-dependent interactive effects. Exposure to hypercapnia singly or in combination with PFOS produced modulations of the biotransformation and hypoxic responses that were apparently concentration and time-dependent. Loading plots of principal component analysis (PCA) produced a significant grouping of individual scores according to the exposure scenarios at day 6 and 9. Overall, the PCA analysis produced a unique clustering of variables that signifies a positive correlation between exposure to high PFOS concentration and expression of E2 responsive genes. Notably, this pattern was not evident for individuals exposed to PFOS concentrations in combination with elevated CO2 scenarios. To our knowledge, the present study is the first of its kind, to evaluate such effects using combined exposure to a perfluoroalkyl sulfonate and elevated levels of CO2 saturation, representative of future oceanic climate change, in any fish species or lower vertebrate.

show abstract

Environmental doses of perfluorooctanoic acid change the expression of genes in target tissues of common carp

Rotondo

Giari

Guerranti

et al. 2018

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

We aimed to evaluate the effects of environmental doses of perfluorooctanoic acid (PFOA) on bioconcentration and gene expression in common carp (Cyprinus carpio). Adult male and female carp were exposed to environmental (200 ng/L) and experimental (2 mg/L) doses of PFOA for 56 d. Carp exposed to 200 ng/L had levels of PFOA below the level of detection in all tissue samples analyzed, whereas variable concentrations were measurable in various tissues from carp exposed to 2 mg/L. The expression level of the glutathione S-transferase (GST) gene, coding for a detoxifying enzyme, increased in a PFOA dose-dependent manner in liver tissues from 200 ng/L to 2 mg/L exposure (p < 0.05). The expression levels of CYP19A gene, coding for the enzyme that converts testosterone into estrogen, were altered in gonadal tissues from male and female carp exposed to either 200 ng/L or 2 mg/L; expression increased in male gonads and decreased in female gonads. Unexpectedly, the expression levels of CYP19A in male and female gonads from carp exposed to 200 ng/L or 2 mg/L were similar (p > 0.05). Therefore, even though environmental doses of PFOA did not accumulate in tissues of the common carp, they did affect the gene expression levels of GST in the liver and CYP19A in the gonads. These observations raise concerns that exposure to environmental doses of PFOA may affect gene expression in animals and possibly in humans, with important health consequences. Environ Toxicol Chem 2018;37:942-948. © 2017 SETAC.

show abstract

Tissue bioaccumulation patterns, xenobiotic biotransformation and steroid hormone levels in Atlantic salmon (Salmo salar) fed a diet containing perfluoroactane sulfonic or perfluorooctane carboxylic acids

Cited by 59 publications

References 66 publications

Modulation of Membrane Lipid Composition and Homeostasis in Salmon Hepatocytes Exposed to Hypoxia and Perfluorooctane Sulfonamide, Given Singly or in Combination

Modulation of Membrane Lipid Composition and Homeostasis in Salmon Hepatocytes Exposed to Hypoxia and Perfluorooctane Sulfonamide, Given Singly or in Combination

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

Environmental doses of perfluorooctanoic acid change the expression of genes in target tissues of common carp

Contact Info

Product

Resources

About