Tissue clearing technique: Recent progress and biomedical applications

Tian, Ting; Yang, Zhaoyang; Li, Xiaoguang

doi:10.1111/joa.13309

Cited by 104 publications

(78 citation statements)

References 118 publications

(259 reference statements)

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“…The use of tissue clearing in human health—and more particularly for respiratory diseases—emerges and has already been reported for the analysis of lung organoids or lung biopsies from human patients [ 39 , 57 ]. In combination with biochemistry and histology, tissue clearing and 3D imaging will contribute to personalized medicine [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution

Frétaud

Descamps

Laubreton

et al. 2021

Viruses

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Background: Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis of RSV infection is required. To date, the pathophysiology of the disease and its diagnosis has mostly relied on chest X-ray and genome detection in nasopharyngeal swabs. The development of new imaging approaches is instrumental to further the description of RSV spread, virus–host interactions and related acute respiratory disease, at the level of the entire lung. Methods: By combining tissue clearing, 3D microscopy and image processing, we developed a novel visualization tool of RSV infection in undissected mouse lungs. Results: Whole tissue analysis allowed the identification of infected cell subtypes, based on both morphological traits and position within the cellular network. Furthermore, 3D imaging was also valuable to detect the cytoplasmic viral factories, also called inclusion bodies, a hallmark of RSV infection. Conclusions: Whole lung clearing and 3D deep imaging represents an unprecedented visualization method of infected lungs to allow insight into RSV pathophysiology and improve the 2D histology analyses.

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Section: Discussionmentioning

confidence: 99%

New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution

Frétaud

Descamps

Laubreton

et al. 2021

Viruses

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“…Using the tissue clearing technique, several methods are available for 3D imaging 7 . Immunostaining is advantageous as it allows the staining of specific proteins using antibodies.…”

Section: Discussionmentioning

confidence: 99%

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Seya

Mukunoki

et al. 2021

JoVE

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Blood vessels are complex networks with tree-like structures, and vascular networks are essential for maintaining both circulation and maintaining organ function.Clarifying the mechanism of blood vessel formation is therefore extremely useful for elucidating developmental processes and pathological mechanisms. Murine hind-limb vessels are often used as a model for physiological and pathological angiogenesis.Evaluation is mainly performed via a two-dimensional method using tissue sections.However, methods for evaluating three-dimensional (3D) vascular morphology are particularly limited. This paper introduces a method for visualizing murine hind-limbs using computed tomography (CT). Radiation-opaque resin is injected through the descending aorta, and whole vessels are filled with dye. By adjusting the time of dye injection, arterial-specific filling is also possible, and samples can be obtained with any micro-X-ray CT device. This contrast method provides a basic technique for the 3D evaluation of murine blood vessels in the lower extremities. Furthermore, this method can be used to visualize all blood vessels below the diaphragm and evaluate blood vessels in the abdominal organs.

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“…However, the main focus of tissue clearing methods is in neuroscience and brain development. The majority of original papers and reviews in tissue clearing as well as 3D brain CUBIC-atlas come from this field (Murakami et al, 2018;Tian et al, 2021;Ueda et al, 2020;Vigouroux et al, 2017). However, the number of publications discussing tissue clearing methods on heart is limited (Kolesova et al, 2016;Nehrhoff et al, 2016;Yokoyama et al, 2017), even though the field of heart and vasculature development has its avid audience.…”

Section: Tissue Clearing Methods Optimized For Heart and Vessel Imagingmentioning

confidence: 99%

Tissue clearing and imaging methods for cardiovascular development

et al. 2021

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Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different tissue clearing methods for 3D imaging of heart and vasculature, based on chemical composition (solvent-based, simple immersion, hyperhydration, and hydrogel embedding techniques). We discuss in detail compatibility of various tissue clearing techniques with visualization methods: fluorescence preservation, immunohistochemistry, nuclear staining, and fluorescent dyes vascular perfusion. We also discuss myocardium visualization using autofluorescence, tissue shrinking, and expansion. Then we overview imaging methods used to study cardiovascular system and live imaging. We discuss heart and vessels segmentation methods and image analysis. The review covers the whole process of cardiovascular system 3D imaging, starting from tissue clearing and its compatibility with various visualization methods to the types of imaging methods and resulting image analysis.

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Tissue clearing technique: Recent progress and biomedical applications

Cited by 104 publications

References 118 publications

New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution

New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Tissue clearing and imaging methods for cardiovascular development

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