Tissue culture and Agrobacterium-mediated genetic transformation studies in four commercially important indica rice cultivars

Sundararajan, Sathish; Balaji, S.; Venkatesh, R.; Ramalingam, Sathishkumar

doi:10.1007/s12892-017-0045-0

Cited by 19 publications

(10 citation statements)

References 24 publications

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“…Asimismo, los callos embriogénicos de la variedad indica Ratnagiri-711 de 35 días de edad obtuvieron eficiencia de transformación de 34,03 %, mientras que al utilizar callos de veinticinco días se dio una reducción considerable en la eficiencia de la transformación 8,86 % (Sawant et al, 2018). En otro caso, se utilizó la variedad ASD16, en donde fueron los callos de veintiún días de edad los que mostraron mayor frecuencia de transformación, en contraste con los callos de catorce días (Sundararajan et al, 2017). Este estudio evidencia un efecto de la concentración de la acetosiringona sobre la eficiencia de transformación con Agrobacterium, ya que al duplicar la concentración de 76 µM a 150 µM se incrementó la expresión transitoria del gen GusPlus pero decayó al utilizar la concentración de 300 µM.…”

Section: Discussionunclassified

Aspectos clave para la transformación genética de arroz (Oryza sativa L.) subespecie indica mediante Agrobacterium tumefaciens

et al. 2021

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Introducción. La transformación del arroz (Oryza sativa L. ssp indica) mediada por Agrobacterium, representa una oportunidad para la investigación científica y el mejoramiento genético. Es necesaria la optimización del protocolo para obtener la mayor eficiencia de transformación. Objetivo. Evaluar diferentes factores que afectan la transformación genética en callos embriogénicos de arroz de la subespecie indica vía Agrobacterium tumefaciens. Materiales y métodos. Este estudio se realizó en San José, Costa Rica, entre 2012 y 2014. En seis tratamientos se evaluaron: el efecto de la edad del callo, la concentración de acetosiringona, condición luminosa, la presencia o ausencia de radícula y la cepa de Agrobacterium tumefaciens en la transformación genética de callos embriogénicos de arroz de la variedad CR5272 con el gen reportero gus. Se compararon la cepa de Agrobacterium LBA4404 con el plásmido pCAMBIA1305.2 y las cepas ATHV, GV3101 y LBA4404, con el plásmido pCAMBIA1303; mediante pruebas histoquímicas para la detección de la expresión transitoria del gen marcador que codifica para la β-glucuronidasa. Resultados. La evaluación de los seis tratamientos con la cepa LBA4404::pCAMBIA1305.2 resultó en expresión transitoria del gen GusPlus de 1,33-7,00 % para la variedad CR5272 y de 8,00 % para el control con la variedad Nipponbare (ssp. japonica). Las cepas con el plásmido pCAMBIA1303 presentaron una expresión transitoria del gen gusA entre el 100-65 % con un área promedio de 14,23 mm2 (ATHV), 8,81 mm2 (GV3101), y 8,83 mm2 (LBA4404), sin diferencias significativas entre ellas; sin embargo, sí hubo diferencias al compararlas con la cepa LBA4404::pCAMBIA1305.2 (85 %, 4,39 mm2). Conclusiones. La utilización de las condiciones: callos de seis días, concentración de acetosiringona de 76 µM, luz antes y después del cocultivo, presencia de radícula y la cepa ATHV::pCAMBIA 1303, mejoraron la eficiencia de transformación con Agrobacterium tumefaciens en la variedad de arroz CR5272.

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Section: Discussionunclassified

Aspectos clave para la transformación genética de arroz (Oryza sativa L.) subespecie indica mediante Agrobacterium tumefaciens

et al. 2021

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“…Ace) ovary tissues (Ramsay et al, 2003). However, N6 medium induced higher percentages of callus and green plants than did MS medium for rice (O. sativa; Sundararajan et al, 2017). For Indian siris (Albizia lebbeck), the WPM medium achieved the highest primary somatic embryoids development, whereas enhanced maturation of primary somatic embryoids occurred on MS medium (Saeed and Shahzad, 2015).…”

Section: Basal Culture Mediummentioning

confidence: 99%

“…The ability of plant regeneration is affected by multiple factors, including use of a plant growth regulator (PGR; Çabuk and Özgen, 2016 ; Gerdakaneh et al, 2020 ), the composition of basic medium ( Sundararajan et al, 2017 ; Chimdessa, 2020 ), and explant type ( Dhar and Joshi, 2005 ; Minutolo et al, 2020 ). Importantly, plant tissue culture presents strong species dependence and genotype specificity.…”

Section: Introductionmentioning

confidence: 99%

New Insights Into Tissue Culture Plant-Regeneration Mechanisms

et al. 2022

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Plant regeneration occurs when plants repair or replace damaged structures based on the totipotency and pluripotency of their cells. Tissue culture is one of the most widely used regenerative technologies. Recently, a series of breakthroughs were made in the study of plant regeneration. This review summarizes two regenerative pathways in tissue culture: somatic embryogenesis and de novo organogenesis. Furthermore, we review the environmental factors influencing plant regeneration from explant sources, basal culture medium, plant growth regulators, and light/dark treatment. Additionally, we analyse the molecular mechanisms underlying two pathways. This knowledge will promote an understanding of the fundamental principles of plant regeneration from precursor cells and lay a solid foundation for applying plant micropropagation and genetic modification.

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“…Furthermore, suitable transformation systems for gene delivery and expression in plant cells, such as Agrobacterium -mediated, particle bombardment, and biolistic methods, are necessary. Agrobacterium -mediated transformation is one of the most commonly used methods for plant transformation − because it is highly effective for the stable integration of the transgene into the plant genome with low transgene copy number …”

Section: Strategies For Multifold Enhancement Of Vitamin E Content In...mentioning

confidence: 99%

Antioxidant Green Factories: Toward Sustainable Production of Vitamin E in PlantIn VitroCultures

2023

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Vitamin E is a dietary supplement synthesized only by photosynthetic organisms and, hence, is an essential vitamin for human well-being. Because of the ever-increasing demand for natural vitamin E and limitations in existing synthesis modes, attempts to improve its yield using plant in vitro cultures have gained traction in recent years. With inflating industrial production costs, integrative approaches to conventional bioprocess optimization is the need of the hour for multifold vitamin E productivity enhancement. In this review, we briefly discuss the structure, isomers, and important metabolic routes of biosynthesis for vitamin E in plants. We then emphasize its vital role in human health and its industrial applications and highlight the market demand and supply. We illustrate the advantages of in vitro plant cell/tissue culture cultivation as an alternative to current commercial production platforms for natural vitamin E. We touch upon the conventional vitamin E metabolic pathway engineering strategies, such as single/ multigene overexpression and chloroplast engineering. We highlight the recent progress in plant systems biology to rationally identify metabolic bottlenecks and knockout targets in the vitamin E biosynthetic pathway. We then discuss bioprocess optimization strategies for sustainable vitamin E production, including media/process optimization, precursor/elicitor addition, and scale-up to bioreactors. We culminate the review with a short discussion on kinetic modeling to predict vitamin E production in plant cell cultures and suggestions on sustainable green extraction methods of vitamin E for reduced environmental impact. This review will be of interest to a wider research fraternity, including those from industry and academia working in the field of plant cell biology, plant biotechnology, and bioprocess engineering for phytochemical enhancement.

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Tissue culture and Agrobacterium-mediated genetic transformation studies in four commercially important indica rice cultivars

Cited by 19 publications

References 24 publications

Aspectos clave para la transformación genética de arroz (Oryza sativa L.) subespecie indica mediante Agrobacterium tumefaciens

Aspectos clave para la transformación genética de arroz (Oryza sativa L.) subespecie indica mediante Agrobacterium tumefaciens

New Insights Into Tissue Culture Plant-Regeneration Mechanisms

Antioxidant Green Factories: Toward Sustainable Production of Vitamin E in PlantIn VitroCultures

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