Tissue culture and micropropagation of tree peony (Paeonia suffruticosa Andr.)

Silva, Jaime A. Teixeira da; Shen, Miaomiao; Yu, Xiaonan

doi:10.1007/s12892-012-0040-z

Cited by 21 publications

(15 citation statements)

References 14 publications

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“…These man-made forest fires are also a major source of pollutants including black carbon which is regarded as a major cause of glacier melt in the Himalaya 5 , and has the capacity to influence regional climate 6 . Fire regime the world over is likely to change in many regions because of the global climate change and change in land-use patterns 1 .…”

Section: Nature Of Forest Fires Inmentioning

confidence: 99%

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Histological Analysis of Somatic Embryogenesis in Itoh Peony

2016

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Itoh peony is one of the popular ornamental plants, which represents an intermediate form between herbs and trees. The present study was aimed at developing a culture growing system for mass multiplication of Itoh peony cv. 'Julia Rose'. Axillary buds were cultured on Murashige and Skoog medium with 6-benzylaminopurine and indole-3-butyric acid for indirect somatic embryogenesis. Nodular callus was derived from the base of the axillary buds. Histological analysis was performed to elucidate the origin and development of somatic embryos.

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Section: Nature Of Forest Fires Inmentioning

confidence: 99%

“…An alternative method of vegetative propagation is in vitro micropropagation. The main technique of micropropagation is direct organogenesis induction 5,6 . Axillary buds and leaves were used as explants [7][8][9] .…”

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confidence: 99%

Histological Analysis of Somatic Embryogenesis in Itoh Peony

2016

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“…A feasible and optimal protocol for the somatic embryogenesis of P. ostii 'Fengdan' is still not yet available, even though some research has been done [15]. The aim of this study is to develop efficient plant regeneration protocols via somatic embryogenesis using zygotic embryos, hypocotyls, and cotyledons as the explants.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Ren

Jeong

2019

Plants

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Somatic embryogenesis is a preferred method for vegetative propagation due to its high propagation efficiency. In this study, zygotic embryos, cotyledons, and hypocotyls of Paeonia ostii ‘Fengdan’ were used as the explant to induce somatic embryogenesis. The results showed that a combination of 0.5 mg·L−1 thidiazuron (TDZ) and 0.5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing somatic embryos from the zygotic embryo and cotyledon explants. Hypocotyls only formed somatic embryos on Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 TDZ and 0.5 mg·L−1 1-naphthylacetic acid (NAA). Moreover, the compact callus was effectively produced from zygotic embryo, cotyledon, and hypocotyl explants in medium supplemented with a combination of 3.0 mg·L−1 6-benzylaminopurine (BA) and 1.0 mg·L−1 NAA, and then converted into somatic embryos in the same medium, and the ratio of the explants with embryo induction and number of embryos induced per explant were much higher than those induced by 0.5 mg·L−1 TDZ and either 0.5 mg·L−1 2,4-D or 0.5 mg·L−1 NAA. The MS medium was better than the woody plant medium (WPM) for inducing somatic embryos from zygotic embryo and hypocotyl explants, whereas the WPM was better than the MS medium for somatic embryogenesis induction from cotyledon explants. All of the somatic embryos developed well into mature embryos on their respective media supplemented with both 3.0 mg·L−1 BA and 1.0 mg·L−1 NAA. Overall, the protocols for indirect somatic embryogenesis from zygotic embryo, cotyledon, and hypocotyl of P. ostii ‘Fengdan’ were successfully established, which can greatly facilitate their propagation and breeding processes.

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“…This review provides a compilation of important studies that show progress in research related to Paeonia molecular biology, and assesses molecular markers, genomics, transcriptomics, proteomics, metabolomics, and micro-RNA studies, research related to functional genes, as well as molecular techniques used for biological testing. Many molecular techniques in Paeonia research are closely linked to the use of in vitro biotechnology, tissue culture, and micropropagation (Shen et al 2012;Teixeira da Silva et al 2012). One of the objectives of this review is to provide a compilation of the available literature to guide future studies of Paeonia molecular biology.…”

Section: Introductionmentioning

confidence: 99%

Advances in molecular biology of Paeonia L.

Fan

Wang

Dong

et al. 2019

Planta

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Molecular biology can serve as a tool to solve the limitations of traditional breeding and cultivation techniques related to flower patterns, the improvement of flower color, and the regulation of flowering and stress resistance. These characteristics of molecular biology ensured its significant role in improving the efficiency of breeding and germplasm amelioration of Paeonia. This review describes the advances in molecular biology of Paeonia, including: (1) the application of molecular markers; (2) genomics, transcriptomics, proteomics, metabolomics, and microRNA studies; (3) studies of functional genes; and (4) molecular biology techniques. This review also points out select limitations in current molecular biology, analyzes the direction of Paeonia molecular biology research, and provides advice for future research objectives.

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Tissue culture and micropropagation of tree peony (Paeonia suffruticosa Andr.)

Cited by 21 publications

References 14 publications

Histological Analysis of Somatic Embryogenesis in Itoh Peony

Histological Analysis of Somatic Embryogenesis in Itoh Peony

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Advances in molecular biology of Paeonia L.

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