Tissue culture approaches for the selection of aluminium-tolerant<i>Citrus</i>species in the Mekong Delta of Vietnam

Toan, Nguyen Bao; Ve, Nguyen Bao; Debergh, Pierre

doi:10.1080/14620316.2004.11511865

Cited by 5 publications

(3 citation statements)

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“…Al toxicity has several consequences of, including root growth inhibition, oxidative stress as a result of reactive oxygen species (ROS) generation, alteration of cell wall and plasma membrane characteristics, nutrient unbalances, cytoplasmic Ca 2 + efflux and induction of callose (1,3- β -D-glucan) formation [26, 37, 38, 41]. The use of in vitro tissue culture technique is suitable to study the physiological effects of Al and allows the application of cells with uniform growth and the investigation of physiological and biochemical Al toxicity at the cellular level [20, 39, 45]. The negative effects of Al toxicity in cultured cells for some plant species, such as tomato [28], tobacco [50], wheat [11], Citrus species [46] and Lobelia chinensis [18], have been investigated.…”

Section: Introductionmentioning

confidence: 99%

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Awad

Salih

Khalaf

et al. 2019

Journal of Genetic Engineering and Biotechnology

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BackgroundAl is a common metallic element found in earth's crust and is a toxic pollutant present at high concentrations in acidic soil, thus affecting plant growth. Despite being well studied as a toxic element, the effects of Al on date palm have not been investigated. This study aimed to assess the toxic effects of different Al concentrations on the development and growth of date palm callus and evaluate the biochemical and molecular response of date palm cells under Al stress.ResultsOur study revealed the phytotoxicity of Al concentrations (50, 100, 150 and 200 mg.l-1) on date palm callus. The fresh and dry weight and the number of produced embryos were significantly decreased in response to Al concentration. At 150 mg.l-1, the embryo number decreased to 1.66 compared with the 19.33 in the control treatment. At high Al concentration (200 mg.l-1), the callus failed to produce any embryo. Biochemical analysis revealed that Al exposure had negative effect on callus. Total soluble carbohydrates, total soluble protein and free amino acids were decreased in plants receiving 200 mg.l-1 Al treatment compared with those in the untreated ones. A similar decline was observed in total soluble protein and free amino acid in response to Al treatment. Significant accumulations of malondialdehyde, H2O2 and peroxidase activity accompanied the increase in Al concentration in cultured tissues, revealing the generation of toxic reactive oxygen species in affected cultures. The genotoxic effect of Al at high concentrations (150 and 200 mg.l-1) was revealed by protein patterns.ConclusionOur findings revealed for the first time the phytotoxicity of Al to date palm callus. At 200 mg.l-1, Al prevented the embryo production of date palm callus. At 50, 100, 150 and 200 mg.l-1, Al negatively affected the biochemical characteristics of date palm callus. At 150 and 200 mg.l-1, Al induced changes in protein expression. These data showed that the tissue culture technique can be used as a valuable approach in heavy metal toxicity studies.

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Section: Introductionmentioning

confidence: 99%

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Awad

Salih

Khalaf

et al. 2019

Journal of Genetic Engineering and Biotechnology

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“…In addition, it was observed that low concentration of Al was detrimental to the growth of cell cultures of Medicago sativa [20], tobacco [18,19] and carrot [42]. Al toxicity could cause the death of the non aluminium-tolerant cell line of Citrus species [22].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the fresh weight of Nicotiana plumbaginifolia callus tissues was found decreased with the elevated concentrations of Al2(SO4)3 [18,19]. Al toxicity was found to depress the cells growth of Medicago sativa [20] and the growth of protocorm-like bodies of banana [21] as well as caused the death of the non aluminium-tolerant cell line of Citrus species [22]. [23] noticed that there was a significant reduction of the frequency of embryoid induction and root regrowth rate of Triticum aestivum on induction media containing aluminium.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2017

JESR

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Abstract:Being the most abundant element on earth, aluminium has become one of the major restricting factors that affect the growth and development of plants. The first target of aluminium toxicity is the root apex which would in turn affect the overall growth of the plants. In this study, the effect of various concentrations of aluminium (Al) on the growth of Lobelia chinensis was determined. Nodal explants of L. chinensis were cultured on MS medium containing various concentrations of aluminium chloride (AlCl3) (0, 1.0, 2.5, 5.0, 7.5, 10.0 and 20 µM) with 1250.0 μM ion phosphate and pH was adjusted to 4.6. It was observed that both the growth of shoot and root decreased with the increasing concentrations of Al.

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Somatic Embryogenesis, In Vitro Selection and Plantlet Regeneration for Citrus Improvement

Kumar

Gill

Gosal

2018

Biotechnologies of Crop Improvement, Volume 1

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Tissue culture approaches for the selection of aluminium-tolerantCitrusspecies in the Mekong Delta of Vietnam

Cited by 5 publications

References 0 publications

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

Phytotoxic and genotoxic effect of Aluminum to date palm (Phoenix dactylifera L.) in vitro cultures

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Somatic Embryogenesis, In Vitro Selection and Plantlet Regeneration for Citrus Improvement

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