Tissue Culture Independent Agrobacterium tumefaciens Mediated In Planta Transformation Method for Tropical Maize (Zea mays.L)

Abhishek, Alok; Kumari, Rita; Karjagi, Chikkappa G.; Kumar, Pradyumn; Kumar, B. M. Vijay; Dass, Sain; Kumar, Rajesh; Ramteke, Pramod W.

doi:10.1007/s40011-014-0454-0

Cited by 13 publications

(6 citation statements)

References 17 publications

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“…In some protocols, a selection step under in vivo conditions (e.g. maize [133]) or in vitro conditions using soilrite as a medium (e.g. roselle/Hibiscus sabdariffa [134]) has been added after inoculation to select the best-performing T 0 chimeric plants.…”

Section: Shoot Apical Meristem Injury Under In Vivo Conditionsmentioning

confidence: 99%

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformation slows scientific progress for a large range of crops that are essential for food security on a global scale. Over the years, novel stable transformation strategies loosely grouped under the term “in planta” have been proposed and validated in a large number of model (e.g. Arabidopsis and rice), major (e.g. wheat and soybean) and minor (e.g. chickpea and lablab bean) species. The in planta approach is revolutionary as it is considered genotype-independent, technically simple (i.e. devoid of or with minimal tissue culture steps), affordable, and easy to implement in a broad range of experimental settings. In this article, we reviewed and categorized over 300 research articles, patents, theses, and videos demonstrating the applicability of different in planta transformation strategies in 105 different genera across 139 plant species. To support this review process, we propose a classification system for the in planta techniques based on five categories and a new nomenclature for more than 30 different in planta techniques. In complement to this, we clarified some grey areas regarding the in planta conceptual framework and provided insights regarding the past, current, and future scientific impacts of these techniques. To support the diffusion of this concept across the community, this review article will serve as an introductory point for an online compendium about in planta transformation strategies that will be available to all scientists. By expanding our knowledge about in planta transformation, we can find innovative approaches to unlock the full potential of plants, support the growth of scientific knowledge, and stimulate an equitable development of plant research in all countries and institutions.

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Section: Shoot Apical Meristem Injury Under In Vivo Conditionsmentioning

confidence: 99%

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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“…However, the transformation frequency was reported to be quite low and the screening of the transformants by PCR analysis was time-consuming. Abhishek et al (2014b) developed a tissue culture independent protocol for in planta transformation in tropical maize by using plumular meristems of germinating seeds as explants and transforming them using Agrobacterium approach.…”

Section: Transformation Techniquesmentioning

confidence: 99%

Advances in Maize Transformation Technologies and Development of Transgenic Maize

et al. 2017

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Maize is the principal grain crop of the world. It is also the crop where genetic engineering has been employed to a great extent to improve its various traits. The ability to transform maize is a crucial step for application of gene technology in maize improvement. There have been constant improvements in the maize transformation technologies over past several years. The choice of genotype and the explant material to initiate transformation and the different types of media to be used in various stages of tissue culture can have significant impact on the outcomes of the transformation efforts. Various methods of gene transfer, like the particle bombardment, protoplast transformation, Agrobacterium-mediated, in planta transformation, etc., have been tried and improved over years. Similarly, various selection systems for retrieval of the transformants have been attempted. The commercial success of maize transformation and transgenic development is unmatched by any other crop so far. Maize transformation with newer gene editing technologies is opening up a fresh dimension in transformation protocols and work-flows. This review captures the various past and recent facets in improvement in maize transformation technologies and attempts to present a comprehensive updated picture of the current state of the art in this area.

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“…The establishment of a sesame regeneration system has been problematic, and therefore, sesame was labeled a recalcitrant plant, 13 , 14 with a minimal-successful rate of genetic modification. 14 , 16–18 , 19 , 20 In the present work, we report a new, more straightforward, non-tissue culture-based method for rapid and stable transformation of sesame seeds using the ‘imbibed seed co-cultivation method’ (ISCM).…”

Section: Introductionmentioning

confidence: 99%

Stable In-Planta Transformation System For Egyptian Sesame ( Sesamum indicum L.) cv. Sohag 1

Sultan

Tawfik

2023

GM Crops & Food

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Sesame ( Sesamum indicum L.) is an important oil crop and one of the oldest-known oil crops to humankind. Sesame has excellent nutritional and therapeutic properties; it is rich in important fatty acids, protein, fiber, and vital minerals. Oil percentage varies among different genotypes but generally accounts for more than 50% of the seed’s dry weight. To meet the increasing demand for vegetable oil production worldwide, expanding the cultivation of oil crops in newly reclaimed areas worldwide is essential. Molecular breeding is an expeditious approach for varietal improvement but requires efficient transgenesis. Published sesame transformation methods are highly genus-specific, tedious, and involve preparing and testing different media and explants. We produced transgenic sesame plants using a stable, noninvasive, and robust Agrobacterium tumefaciens transformation method. Leaves and flowers excised from the T 0 plants at different developmental stages were PCR screened, and 61/93 seedlings were found to be PCR positive. T 1 seeds resulting from two lines were germinated in a biocontainment greenhouse facility and screened using PCR, basta leaf painting, and spraying fully matured plants with basta herbicide (0.02 mg/l); non-transgenic segregants and control non-transgenic plants were severely damaged, and eventually died, while transgenic plants were not affected by the Basta spraying. RT-PCR on T1 plants indicated the presence of Bar transcripts in T 1 progeny. Furthermore, RT-PCR using NPTII primers did not result in any amplification in transgenic sesame plants (NPTII is present in the vector but not in the T-DNA region) indicating that the transgenic sesame plants were not an Agrobacterium -contaminant.

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Tissue Culture Independent Agrobacterium tumefaciens Mediated In Planta Transformation Method for Tropical Maize (Zea mays.L)

Cited by 13 publications

References 17 publications

A comprehensive review of in planta stable transformation strategies

A comprehensive review of in planta stable transformation strategies

Advances in Maize Transformation Technologies and Development of Transgenic Maize

Stable In-Planta Transformation System For Egyptian Sesame ( Sesamum indicum L.) cv. Sohag 1

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