Tissue Culture Methods

Oberlander, Herbert

doi:10.1007/978-1-4612-6076-9_7

Springer Series in Experimental Entomology

1980

DOI: 10.1007/978-1-4612-6076-9_7

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Tissue Culture Methods

Herbert Oberlander¹

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1985

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Cited by 27 publications

References 48 publications

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Immune Effector Mechanisms Required for Protection to Rabies Virus

et al. 1995

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Genetically engineered mice with targeted mutations in genes encoding immunologically relevant molecules were used to elucidate the role of different immune effector mechanisms in protection against a rabies virus (RV) infection. In vaccinated animals challenged with a highly virulent strain of RV, antibodies were crucial in protection. In naive mice challenged with an attenuated strain of the virus that does not cause disease in adult fully immunocompetent mice but kills RAG mice that lack functionally active T and B cells, different immune effector mechanisms were shown to suffice for protection.

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Immune Effector Mechanisms Required for Protection to Rabies Virus

et al. 1995

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Postmolt cuticle growth in a cockroach: in vitro deposition of multilamellate and circadian-like layered endocuticle

Weber

1985

Experientia

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Insect tissue culture systems: models for study of hormonal control of development

Porcheron

1991

In Vitro Cell Dev Biol - Animal

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The regulation of growth and development of insects is under endocrine control and involves both juvenile hormones and ecdysteroids. Neuropeptides are master regulators which control the secretion of these hormones. Most experiments in insect endocrinology have been conducted in vivo, but tissue culture methodology is playing an increasing role due to the great interest in simpler model systems for the study of complex processes that occur in vivo. The availability of appropriate media has allowed the culture of a variety of insect organs and cell lines of defined origin which have kept certain properties of the parent tissues. Tissue culture approaches have been useful for studying hormonal control of morphogenetic processes. Cell lines are particularly suited to the study of hormonally regulated mechanisms of macromolecular biosynthesis and gene expression. Thus, the value of in vitro analysis in studies of regulation of hormone production is now recognized. Results obtained from tissue culture allow more precise definition of the hormonal requirements of insect cells and tissues for growth and differentiation and might make possible the discovery of new growth regulators.

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Tissue Culture Methods

Cited by 27 publications

References 48 publications

Immune Effector Mechanisms Required for Protection to Rabies Virus

Immune Effector Mechanisms Required for Protection to Rabies Virus

Postmolt cuticle growth in a cockroach: in vitro deposition of multilamellate and circadian-like layered endocuticle

Insect tissue culture systems: models for study of hormonal control of development

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