Tissue Culture of Dillenia Philippinensis Rolfe

Lumeran, Belen T.

doi:10.17148/iarjset.2016.3921

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…this species has many uses and is rather difficult to find in the wild. According to Lumeran (2016), their seeds do not germinate in its natural habitat due to its hard seed coat. The fruits are utilized as hair cleanser, an excellent source of jam, sauce, and fish flavourings.…”

Section: Conservation Valuementioning

confidence: 99%

Plant Collection in Bogor Botanical Garden as a Source of Functional Food

Hidayat¹

2021

Medkon

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Plant collection in Bogor Botanical Garden (BBG) has various potentials, one of which is as a food source. However, not all food produced from plants can be claimed as functional foods. This study analyses the potential of plants kept in the collections of BBG as functional foods and medicine sources with nutritional and health values. Literature studies have been carried out related to nutrition value and active substances owned by these plants, while data related to health claim was based on the results of ethnobotany studies, especially the use of medicinal plants by 28 ethnics in Indonesia. The results suggest 168 species of the BBG collection which can be claimed as a source of functional food which can be categorized into seven main categories. Among these plants are species classified as threatened or endangered such as Mangifera casturi, Vanilla planifolia, Pterocarpus indicus, Borassus flabellifer, Cycas rumphii, Dillenia philippinensis, and Litchi chinensis. Key words: BBG, functional food, threatened species

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Section: Conservation Valuementioning

confidence: 99%

Plant Collection in Bogor Botanical Garden as a Source of Functional Food

Hidayat¹

2021

Medkon

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“…Kalus kompak tersebut muncul dari daerah hipokotil kecambah biji yang dibiarkan tumbuh cukup lama pada media WP yang mengandung 1 mg/l BAP. Pada penelitian Lumeran (2016), kalus kompak terbentuk dengan eksplan akar kecambah in vitro pada media MS dengan penambahan berbagai kombinasi konsentrasi BAP dan NAA. Tunas dihasilkan dari regenerasi optimal kalus yang ditanam di media yang mengandung 1 mg/l BAP dan 1 mg/l NAA.…”

Section: B Pembahasanunclassified

“…Kultur jaringan D. phillipinensis juga telah dilakukan oleh Lumeran (2016) melalui perkecambahan in vitro biji pada media Knudson C yang ditambahkan BAP serta NAA, setelah tumbuh kalus, dipindahkan ke media MS yang mengandung BAP serta NAA. Media perbanyakan tunas terbaik adalah MS yang ditambahkan BAP dan NAA pada konsentrasi yang sama yaitu 1 mg/l, namun tunas yang terbentuk masih tidak berbeda nyata dengan 0,5 mg/l dan pengamatan tentang morfologi tunas belum dilakukan.…”

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“…Perbanyakan tunas dari kalus kompak yang mengandung calon tunas dan peningkatan ketegaran eksplan dengan GA3 telah dilakukan oleh Wulandari et al, (2019b). Tujuan dari penelitian ini adalah untuk mengetahui respon tumbuh dan morfologi kultur tunas sempur dengan menggunakan modifikasi media hasil penelitian Lumeran (2016), yaitu menggunakan kombinasi BAP dan NAA dengan konsentrasi 1-2mg/l dan 0,5-1 mg/l, dari eksplan tunas pucuk kultur in vitro.…”

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PERTUMBUHAN DAN MORFOLOGI KULTUR TUNAS SEMPUR (Dillenia philippinensis Rolfe) PADA MEDIA MS-BAP-NAA (Growth and Morphology of Sempur (Dillenia philippinensis Rolfe) Shoot Culture on MS –BAP-NAA Media)

Rantau

Wulandari

Ermayanti

et al. 2021

Jur.pen.hut.tanaman

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Propagation of sempur (Dillenia philippinensis Rolfe) using conventional vegetative methods is considered ineffective due to slow process and low germination level. Propagation by tissue culture is considered more effective because it does not depend on the season and requires less plant material. It can benefit sempur conservation since it is categorized as threatened with extinction in 2020 on the IUCN red list. This study aimed to evaluate the effect of BAP and NAA on the growth of sempur's shoots and to observe the morphology of shoot culture. The media used as a control was MS without growth regulators. The treatment medium was MS with the addition of BAP and NAA. Shoots were used as explants. Shoot growth and plantlet morphology were observed eight weeks after planting. The results indicated that the combination of 1 mg/l BAP and 0.5 mg/l NAA resulted in the highest total number of leaves, nodes and adventitious shoots. The combination of BAP and NAA, each 1 mg/l, resulted in the highest fresh weight and callus formation. The combination of 1 and 2 mg/l BAP and 0.5-1 mg/l NAA could not form roots. In comparison, the combination of BAP and NAA in culture media could change the shape and size of the leaves. The survival rate of growth of plantlets derived from MS medium was 50% at 22 weeks after acclimatization.

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In vitro growth of Sempur (Dillenia philippinensis Rolfe) shoots in response to different types of plants growth regulators supplemented on MS medium

Wulandari

Rudiyanto

Rantau

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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Plant tissue culture of Sempur (Dillenia philippinensis Rolfe) has not been widely reported. The previous study showed that BAP added to Murashige & Skoog (MS) basal medium promoted the growth of D. philippinensis shoot culture. This research aimed to investigate the growth of D. philippinensis shoot tip cultures on MS medium containing three types of cytokinins namely BAP, Kinetin, and 2-iP compared with Gibberelic acid (GA3). The results showed that the type and concentrations of cytokinins significantly affected all growth parameters observed except the number of roots which was only influenced by cytokinin concentrations alone. However, different levels of GA3 did not significantly affect all growth parameters observed. After eight weeks of culture, MS medium containing 1 mg/l 2-iP gave the best response for plant height, (3.68± 0.26 cm). The highest number of leaves (20.33±3.14) and nodes (6.67± 0.21) were found in MS medium containing 0.5 mg/l BAP. While the highest number of roots (1.83±0.17) was found in MS medium without the addition of cytokinins. The range of average values of plant height at different levels of GA3 was 3.08-3.74 cm; the number of leaves was 5.1-7.5; the number of nodes was 3.9-5.4 and the number of roots was 1.0-2.8.

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Tissue Culture of Dillenia Philippinensis Rolfe

Cited by 3 publications

References 5 publications

Plant Collection in Bogor Botanical Garden as a Source of Functional Food

Plant Collection in Bogor Botanical Garden as a Source of Functional Food

PERTUMBUHAN DAN MORFOLOGI KULTUR TUNAS SEMPUR (Dillenia philippinensis Rolfe) PADA MEDIA MS-BAP-NAA (Growth and Morphology of Sempur (Dillenia philippinensis Rolfe) Shoot Culture on MS –BAP-NAA Media)

In vitro growth of Sempur (Dillenia philippinensis Rolfe) shoots in response to different types of plants growth regulators supplemented on MS medium

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