Tissue Culture of Douglas-Fir and Western North American Conifers

AboEl-Nil, Mostafa

doi:10.1007/978-94-017-0992-7_6

Cited by 5 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, a achieve and unreliable (Minocha 1980; Mott & bioassay employing mung bean seedling cuttings Amerson, 1981;Amerson & Mott, 1982; Bornman, has been widely used to detect rooting factors and 1983;Abo El-Nil, 1987). Spontaneous rooting of cofactors ;Bassuk, Hunter cultured shoots without the aid of growth regulator & Howard, 1981).…”

Section: Introductionmentioning

confidence: 99%

“…Cuttings prepared from young conifer seedlings have frequently been used to study the processes Rooting gymnosperm shoots on tissue culture media involved in root induction (Smith & Thorpe, 1975 a, has been widely recognized to be both difficult to 6; Gronroos & von Arnold, 1987). Similarly, a achieve and unreliable (Minocha 1980; bioassay employing mung bean seedling cuttings Amerson, 1981;Amerson & Mott, 1982; Bornman, has been widely used to detect rooting factors and 1983;Abo El-Nil, 1987). Spontaneous rooting of cofactors ;Bassuk, Hunter cultured shoots without the aid of growth regulator & Howard, 1981).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adventitious root formation in hypocotyl cuttings of Picea sitchensis (Bong.) Carr. — the influence of plant growth regulators

1992

View full text Add to dashboard Cite

SUMMARYA simple method for the study of adventitious root initiation on Sitka spruce [Picea sitchensis (Bong.) Carr.] hypocotyl cuttings is described and was used to examine the effects of plant growth regulators. Twenty-one-dayold seedlings, grown at a low light intensity (15-20 fimol m~^ s~^) to stimulate hypocotyl elongation, provided easy to handle tissues which were responsive to root inducing treatments. Spontaneous rooting only occurred at a low frequency on untreated cuttings and reliable rooting depended upon auxin treatment. When continuous auxin treatment was used, indole-3-butyric acid (IBA) was more effective than either indole-3-acetic acid (IAA) or 1-naphthaleneacetic acid (NAA), and was optimal between lO""" and 10~* M. Pulse treatments with 10~^ M IBA for as little as 10 min were sufficient to stimulate rooting. All other growth regulators examined were inhibitory to IBA induced rooting. 6-benzylaminopurine (BA) was the most active being inhibitory down to a concentration of 3 X 10"' M when it was applied in a mixture with IBA. Pre-treatment of hypocotyl cuttings with BA prior to auxin treatment demonstrated strong cytokinin 'carry-over' inhibitory effects on rooting. Gibberellic acid Ag (GAg), abscisie acid (ABA) and 2-chloroethyl-phosphoric acid (ethrel) were less potent inhibitors of rooting.

show abstract

Section: Introductionmentioning

confidence: 99%