Tissue culture of forest trees: Clonal multiplication of Tectona grandis L. (teak) by tissue culture

Gupta, Pramod Kumar; Nadgir, A. L.; Mascarenhas, A. F.; Jagannathan, V.

doi:10.1016/0304-4211(80)90156-x

Cited by 128 publications

(47 citation statements)

References 10 publications

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“…According to the literature, BAP has been the most commonly used in micropropagation of teak alone or with kinetin [12,13]. High concentration of BAP (5 mg L −1 ) was applied for a few hours [25].…”

Section: Shoot Proliferationmentioning

confidence: 99%

“…However, although numerous authors have been experimenting to establish an efficient, reproducible and simple system for micropropagating teak [11,16,25,27], it still remains problematic due to the poor capacity of shoot proliferation, high susceptibility of shoots to vitrification and browning and the low frequency of in vitro rooting. None of the published protocols fully satisfies the requirements for commercial application in spite of the progress made on tissue culture of teak since the 1970s [8,13,18].…”

Section: Introductionmentioning

confidence: 99%

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Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations

2007

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-Multiple shoots of high quality were produced in vitro from nodal explants of Tectona grandis. An average of about 4 shoots/uninodal explant was obtained within 4 weeks of culture on Murashige and Skoog's (mMS) medium modified by 50% reduction in NH 4 NO 3 concentration, supplemented with benzylaminopurine (1.5 mg L −1 ); indole-3-butyric acid (0.01 mg L −1 ) and gibberellic acid (0.1 mg L −1 ). The latter was applied both in the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L −1 . Hundred percent of shoots rooted cultured on modified MS medium containing IBA (0.5 mg L −1 ) and putrescine (160 mg L −1 ). Putrescine promoted both strong and highly ramified roots and fast growing shoots during the rooting phase, conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a short acclimatization stage in greenhouse where they survived at 100%. This highly reproducible procedure can be adopted for large scale teak propagation. . La présence de la putrescine a permis d'obtenir des racines fortes et ramifiées, des pousses à croissance rapide pendant l'enracinement, donnant des plantes de qualité aptes à une bonne survie. Les plantes ont été endurcies et transférées dans des Jiffy pots pour une acclimatation brève en conditions de serre ayant pour résultat 100% de survie. Ce procédé hautement reproductible peut être adapté à grande échelle dans la propagation de teak du fait de l'efficacité du nombre de pousses obtenues (prolifération), de l'enracinement, de l'acclimatation et du bon développement des plantes. régulateurs de croissance / putrescine / enracinement / explants nodaux / acide gibbérélique

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Section: Shoot Proliferationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations

2007

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“…The of S. suaveolens were placed on MS supplemented with 0.44 µM BAP and 50.0 mg/l CA showed shoot number1.2 ± 0.11, shoot length (mm) 22.9 ± 0.58 and number of nodes per micro shoot 3.06 ± 0.22 and when placed on MS medium supplemented with 0.44 µM BAP and 250.0 mg/l PVP showed shoot number 1.13 ± 0.09, maximum shoot length Table 2). Similarly [22] reported that when PVP is incorporated in the medium as an adsorbent and used to remove the toxic metabolites from the medium. The subcultured stem nodes from in vitro grown plants of Acacia catechu on MS medium adjuvanted with N 6 -benzyladenine and augmented with 1.5 g l -1 PVP to control browning [23].…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Multipurpose Medicinal Tree Stereospermum Suaveolens?Factors Controlling the In Vitro Regeneration

Shukla¹

2013

J Biotechnol Biomaterial

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Stereospermum suaveolens (DC.) is a multipurpose tree endowed with medicinal properties and good timber wood quality. It has analgesic and wound healing property. It is one of the important tree species used in the preparation of ayurvedic formulation known as Dashmula, in which roots of Dashmula tree species are used in preparation of ayurvedic formulations. Wood is employed for construction work, planks and beam, carts, carriages and wagons, furniture, cabinet work and tool handles. The destruction of this plant species is severe and planting of seedlings is almost negligible. Due to low germination and unsuccessful vegetative propagation, in vitro regeneration of Stereospermum suaveolens through micropropagation was studied as a tool for large scale propagation. In the present study, shoots were initiated from the nodal segments of Stereospermum suaveolens further shoot proliferation was achieved on MS medium supplemented with different concentrations of cytokinins and additives.100% root initiation was achieved in the MS medium supplemented with IBA. Different substrates were evaluated during hardening phase. The plants of S. suaveolens grown on coco peat showed 84.6 percent survival and those on soil mixture showed 76.47 percent survival.

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“…Subsiguientemente, explantes libres de agentes contaminantes de entre 1.5 y 2 cm de longitud, fueron transferidos a un medio de cultivo MS de multiplicación, con bencilaminopurina (BAP) (1, 2 y 3 mg L -1 ) y ácido indolbutírico (AIB) (0.5 y 1 mg L -1 ), siguiendo el método descrito por Gupta et al (1980). Se efectuaron tres siembras a intervalos constantes de 21 días.…”

Section: Fases De La Propagación In Vitrounclassified

PROPAGACIÓN CLONAL in vitro DE Swietenia macrophylla King (CAOBA)

Patiño¹,

Troya²,

Morán³

et al. 2014

CyT

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ResumenL a caoba (Swietenia macrophylla) es una especie forestal maderable de múltiples usos, apreciada por su dureza, resistencia, belleza y calidad. La explotación intensiva y un inadecuado sistema de aprovechamiento de la especie han ocasionado la disminución de la variabilidad genética, haciendo imposible la aplicación de programas de mejoramiento genético de caoba en el Ecuador. El cultivo in vitro es una técnica que ayudaría a disminuir este problema mediante la producción de plantas con alta robustez genética. El objetivo de esta investigación fue establecer un método que facilite la propagación in vitro de caoba a partir de segmentos nodales, empleados en la fase de establecimiento con diferentes concentraciones de Ca(ClO) 2 y tiempos de exposición. La contaminación por microorganismos fue controlada con 15 g de Ca(ClO) 2 durante 20 min, alcanzando el 95% de sobrevivencia. Los explantes sanos fueron transferidos a un medio de cultivo MS/2 con distintos niveles de bencilaminopurina (BAP) y ácido indolbutírico (AIB) para su multiplicación in vitro, obteniéndose 70% de brotes con 2 mg L -1 de BAP en combinación con 1 mg L -1 de AIB. Los mejores brotes de la fase de multiplicación se colocaron en medio de cultivo MS/2 con diferentes concentraciones de ANA para facilitar su enraizamiento, ninguno de los tratamientos ensayados permitió generar raíces a los 21 días de su evaluación, aunque el mayor porcentaje de sobrevivencia (65%) se obtuvo al combinar 2 mg L -1 BAP y 1 mg L -1 ANA. Palabras clave: Protocolo, propagación in vitro, Swietenia macrophylla, segmentos nodalesAbstRAct M ahogany (Swietenia macrophylla), is a widely used timber tree species valued for its hardness, strength, beauty and quality. Intensive explotation and inadequate use of this species have resulted in the loss of genetic variability, making impossible the implementation of breeding programs of mahogany in Ecuador. In vitro culture is a promising technique that would help reducing this problem by producing plants with high genetic strength. The objective of this research was to establish a method to facilitate in vitro propagation of mahogany from nodal segments. In order to prevent contamination, nodal segments were treated with Ca(ClO) 2 solution for 20 min. Healthy explants were transferred to MS/2 culture medium with different concentrations of benzylaminopurine (BAP) and indolebutyric acid (IBA) for further in vitro proliferation, obtaining 70% of shoots on medium supplemented with 2 mg L -1 of BAP and 1 mg L -1 IBA. Bud treatment with different concentrations of ANA for in vitro rooting was not efficient, although the greatest survival rate (65%) was obtained on culture medium containing 2 mg L -1 BAP and 1 mg L -1 NAA.

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Tissue culture of forest trees: Clonal multiplication of Tectona grandis L. (teak) by tissue culture

Cited by 128 publications

References 10 publications

Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations

Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations

In vitro Regeneration of Multipurpose Medicinal Tree Stereospermum Suaveolens?Factors Controlling the In Vitro Regeneration

PROPAGACIÓN CLONAL in vitro DE Swietenia macrophylla King (CAOBA)

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