Tissue culture of forest trees—Clonal propagation of mature trees of Eucalyptus citriodora Hook, by tissue culture

Gupta, Pramod Kumar; Mascarenhas, A. F.; Jagannathan, V.

doi:10.1016/0304-4211(81)90262-5

Cited by 95 publications

(39 citation statements)

References 8 publications

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“…The rooting percentage of shoots increased from around 39 to 92% from the first to the third subculture, hence from the 3 rd culture onwards shoots are more suitable for rooting, which may be due to the better physiological condition of shoots for better rooting. Similar results were observed in Eucalyptus L'Heritier (Goncalves 1980;Gupta et al 1981;Badia 1982;Mccomb, Bennett 1982). Also in perennial plants/tree species the growth of the culture may be slow as compared to annual plants, and restore their juvenile traits essential for rooting upon subsequent subcultures, hence this may be one of the reasons for a high rooting percentage in older cultures/3 rd subculture onwards.…”

Section: Discussionsupporting

confidence: 79%

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

Singh¹,

Agarwal²

2016

J. For. Sci.

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A micropropagation protocol was developed by further improvement of prevailing methods using proven germplasm for ex vitro rooting and addressed the effect of the number of subcultures on the rooting ability of shoots. A comparative study was done between in vitro rooting method and ex vitro rooting method. Using the ex vitro rooting method a plantlet could be produced in 135 days, which was in a shorter time compared to the in vitro rooting method -180 days. The best axillary shoot bud induction was observed on Murashige and Skoog (MS) medium supplemented with 4.6 µM thidiazuron (TDZ) with 5 shoot buds per explant. In the shoot cluster, which was subcultured on MS medium supplemented with 2.3 µM TDZ, the rate of shoot multiplication increased in the 3 rd subculture. The maximum mean number of shoots per explant (20) was obtained at the 3 rd subculture on the same medium. Shoots were harvested at the 1 st , 3 rd and 5 th subculture and pulse treated for root induction. The highest rooting (95%) was achieved from the 3 rd subculture onwards with pulse treated shoots for fifteen days. The rooted plants could be established in a greenhouse with 99% survival. Ex vitro rooting is a promising method to reduce the time for plant generation. The resultant plantlets well established in pots and fruiting was observed within a year.

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Section: Discussionsupporting

confidence: 79%

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

Singh¹,

Agarwal²

2016

J. For. Sci.

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“…Zuraida et al (2011) reported that explants cultured on liquid medium supplemented with 1.0 mg L -1 BAP produced the highest number of shoots (31) after 4 weeks when compared with 5 mg L -1 BAP which produced 14 shoots after four weeks of culture. This confirms the assertion that liquid culture explants usually display a higher frequency of growth rate when compared with solid or semi-liquid cultures (Gupta et al, 1981), and maybe as a result of continuous shaking of liquid cultures thus promoting increased intake of the nutrients in the medium. After a second subculture, a 3-fold increase in the average number of shoots (18.3) was recorded for 1.5 mg L -1 BAP at 10 weeks; and at 20 weeks a 28-fold increase was observed (167.6) (Figure 1, Plate 1C).…”

Section: Shoot Proliferationsupporting

confidence: 77%

“…This result agrees with previous report (Firoozabady and Gutterson, 2003), which shows that BAP (0.1 to 0.5 mg L semi-liquid medium (solidified with 1.0 g L -1 Gelrite) in this study maybe due to increased intake of the nutrients made possible by free movement of compounds or nutrients from the culture medium into the plant tissues. Gupta et al (1981) showed that the use of liquid medium facilitates the nutrient uptake by the plant due to better distribution in the culture medium. Similar higher regeneration rates in liquid medium when compared with solid medium have been reported by Alvard et al (1993).…”

Section: Shoot Regenerationmentioning

confidence: 99%

Influence of medium type and growth regulators on in vitro micropropagation of pineapple (Ananas comosus (L.), var. Smooth cayenne)

Akin-Idowu¹,

Akinyemi²,

Ibitoye³

2014

Afr. J. Plant Sci.

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“…4 B Means followed by the same letter do not differ significantly (Tukey, 0.05) (lower-case in columns and upper-case in line) 1 The original data was transformed using (x + 1.0) 1/2 explant in liquid medium was 554.2, which was significantly higher than in solidified medium, with an average of 128.7 shoots per explant. According to Gupta et al (1981), the use of liquid medium facilitates the nutrient uptake by the plant due to its distribution in the culture medium. The higher shoot production in liquid medium was probably favored by the better nutrient uptake.…”

Section: Resultsmentioning

confidence: 99%

Optimization of a protocol for the micropropagation of pineapple

Almeida¹,

Santana²,

Rodriguez

et al. 2002

Rev. Bras. Frutic.

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-The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L.) Merrill) cv. Pérola. Changes in benzylaminopurine (BAP) concentration, type of medium (liquid or solidified) and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%), for three minutes, followed by calcium hypochlorite (2%), for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L -1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m -2 .s -1 ), 27 ± 2°C. After eight weeks, cultures were subcultured for multiplication in MS medium. The following treatments were tested: liquid x solidified medium with different BAP concentrations (0.0, 1.5 or 3.0 mg L -1 ), and the longitudinal cut, or not, of the shoot bud used as explant. The results showed that liquid medium supplemented with BAP at 1.5 mg L -1 , associated with the longitudinal sectioning of the shoot bud used as explant presented the best results, maximizing shoot proliferation. On average, the best treatment would allow for an estimated production of 161,080 plantlets by the micropropagation of the axillary buds of one plant with eight slips and ten buds/slips, within a period of eight months. Index terms:Ananas comosus, tissue culture, in vitro culture, in vitro clonal propagation. OTIMIZAÇÃO DO PROTOCOLO DE MICROPROPAGAÇÃO DO ABACAXIZEIRORESUMO -O objetivo do trabalho foi maximizar o número de brotações, buscando adequar o protocolo de micropropagação do abacaxizeiro-'Pérola', pela manipulação de concentrações de BAP, do estado físico do meio de cultura e do seccionamento das brotações na fase de proliferação. Mudas do tipo filhote foram utilizadas como fonte de explantes, que se constituíram de gemas axilares extraídas após a eliminação das folhas. A desinfestação procedeu-se com álcool 70% (três minutos) e, posteriormente, com hipoclorito de cálcio 2% (quinze minutos). Em seguida, os explantes foram estabelecidos em frascos contendo 15 mL do meio de cultura MS sólido, suplementado com 2,0 mg L -1 de BAP e permaneceram por 60 dias em câmara de crescimento. Na fase de proliferação, estudaram-se os meios de cultura MS sólido e líquido, as concentrações de BAP (0,0; 1,5 e 3,0 mg L -1 ) e o seccionamento longitudinal das brotações. Concluiu-se que o meio MS líquido favoreceu a indução de brotações. O seccionamento das brotações mostrou-se fundamental no aumento da taxa de multiplicação, e a concentração 1,5 mg L -1 de BAP promoveu a melhor resposta para o número de brotações. O trabalho demonstrou que se pode obter até 161.080 plântulas de abacaxi, no final de oito meses, partindo de uma única planta com oito filhotes e dez gemas axilares/filhote. Termos para indexação:Ananas comosus, cultura de tecidos, cultivo in vitro, propagação clonal in vitro.

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Tissue culture of forest trees—Clonal propagation of mature trees of Eucalyptus citriodora Hook, by tissue culture

Cited by 95 publications

References 8 publications

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

Influence of medium type and growth regulators on in vitro micropropagation of pineapple (Ananas comosus (L.), var. Smooth cayenne)

Optimization of a protocol for the micropropagation of pineapple

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