Tissue Culture of Legumes for Crop Improvement

Mroginski, L. A.; Kartha, K.K.

doi:10.1002/9781118060995.ch7

Cited by 47 publications

(11 citation statements)

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“…probably due to the use of inadequate hormones. This lack of regeneration from callus confirms the well-known recalcitrance of grain legumes to in vitro cultures (Mroginski and Kartha, 1984 ;Nagl et al, 1997).…”

Section: Discussionsupporting

confidence: 77%

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

Koné¹,

Kouakou²,

Kone³

et al. 2009

Agronomie Africaine

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A technique was developed to induce callus formation and differentiation using leaf, petiole and root explants from Bambara groundnut [Vigna subterranea (L.) Verdc.]. The combinations and concentrations of different growth regulators were shown to be critical factors in the frequency of callus formation, as well as, in the potential for callus differentiation. It was established that the best responses in callus induction (75 %) were obtained from petiole on a MS (Murashige and Skoog, 1962) basal medium, supplemented with 3 -5 mg/l N-benzylaminopurine (BAP) + 0.5 mg/l α-naphthaleneacetic acid (NAA). Callus growth (GI) was more significant with Ci1 (3.52) and Ci4 (2.76) landraces, while root formation was especially observed with the Ci3 (24.81 %) landrace. Here, for the first time, induction of embryogenic and organogenic callus from petiole, leaf and root of Vigna subterranea (L.) was reported. Both adventitious buds and somatic embryos were initiated after 3 months of subculture on a callus induction medium containing 3-5mg/l BAP + 0.1 -0.5 mg/l ANA. Somatic embryogenesis (50 -68 %) occur more frequently than organogenesis (11 -27 %).Keywords : Callus, bambara groundnut (Vigna subterranea L.), somatic embryogenesis, organogenesis. Ci1 (3,52) et Ci4 (2,76) RESUME FACTEURS INFLUENÇANT L'INDUCTION ET LA DIFFERENCIATION DE CALS Un protocole a été développé pour induire la formation de cals, à partir des explants de feuille, de pétiole et de racine chez le pois de bambara [Vigna subterranea (L.)]. Les combinaisons et les concentrations de différents régulateurs de croissance se sont avérées être des facteurs déterminants dans la fréquence d'induction des cals, de même que pour le potentiel de différenciation des cals. Les résultats montrent également que l'induction des cals a été plus importante avec le pétiole (75 %) sur le milieu de base MS (Murashige et Skoog, 1962) supplémenté avec 3 -5 mg/l de la N-benzylaminopurine (BAP) + 0,5 mg/l de l'acide α-naphthalène acétique (ANA). La croissance des cals (IC) a été plus importante avec les écotypes 16M. KONE et al.

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Section: Discussionsupporting

confidence: 77%

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

Koné¹,

Kouakou²,

Kone³

et al. 2009

Agronomie Africaine

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“…This in vitro regeneration studies were carried out following two earlier reports of Sarker et al (2005) and Sharmin et al (2012) with slight modification. In general pulses have been considered recalcitrant due to their passiveness to in vitro techniques (Mroginski and Kartha 1984). The development of desired transgenic plants in grain legumes is mainly hampered due to the lack of efficient gene delivery system (Nisbet and Webb 1990).…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation

Mubina

Hoque

Sarker

2018

Plant Tissue Cult. & Biotech.

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In vitro regeneration studies compatible to Agrobacterium-mediated genetic transformation were carried out using two different types of zygotic embryo derived explants namely, decapitated embryo (DE) and decapitated embryo with single cotyledon disc (DEC) from three varieties of chickpea (Cicer arietinum L.) such as BARI chhola-4, -5 and -9 cultivated in Bangladesh. The best responses towards in vitro shoot regeneration was obtained from decapitated embryo with DEC on MS containing 0.5 mg/l BAP, 0.5 mg/l Kn and 0.2 mg/l NAA. Healthy and effective roots from the regenerated shoots were developed on MS supplemented with 0.2 mg/l IBA. Genetic transformation was carried out with Agrobacterium strain LBA4404 containing the binary plasmid pCAMBIA1301-PDH45 to integrate salt tolerant PDH45 gene in locally grown varieties of chickpea. The transformed plantlets were successfully established in soil following adequate hardening. Integration of salt tolerant PDH45 gene within the genomic DNA was confirmed through GUS histochemical assay and PCR analysis.

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“…Investigations on in vitro regeneration of Arachis are reported since 1955 (Mroginski and Kartha 1984). In vitro plant regeneration of wild species of Arachis has been achieved from different explant types, including embryonic and mature tissues, through distinct morphogenic pathways, aiming at conservation, multiplication and germplasm availability for improvement programs of the cultivated groundnut (Table 2).…”

Section: Tissue Culture Of Wild Arachis Speciesmentioning

confidence: 99%

Micropropagation and in vitro conservation of wild Arachis species

Pacheco

Gagliardi

Valls

et al. 2009

Plant Cell Tiss Organ Cult

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Wild species of Arachis constitute potential sources of novel genes for groundnut improvement programs and some of them are also considered as new agricultural usage. The majority of these species occur in regions under intensive human activity and their areas of distribution are being drastically reduced, thus requiring effective conservation measures. Conservation of Arachis germplasm is usually carried out in seed banks or as live plants. However, seed renewal can be impaired by loss of germinative potential, and plant multiplication under field conditions can be limited by specific soil and environmental requirements or by low seed yield. Therefore, complementary in vitro methodologies represent an important tool for ex situ conservation of Arachis germplasm. In this work, we analyse the state of art of micropropagation and the use of in vitro conservation methodologies for wild relatives of the groundnut.

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Tissue Culture of Legumes for Crop Improvement

Cited by 47 publications

References 100 publications

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation

Micropropagation and in vitro conservation of wild Arachis species

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