Tissue Culture of Woody Plants and Its Relevance to Molecular Biology

Minocha, Rakesh; Jain, S. Mohan

doi:10.1007/978-94-017-2311-4_13

Cited by 15 publications

(6 citation statements)

References 158 publications

(111 reference statements)

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“…The decline is the most threatening stress on tree species, and the D. sissoo decline leads this tree to the danger of extinction (Orwa et al, 2009;Shah et al, 2010). The use of resistant germplasm to control dieback will be the most effective way to attain the disease's sustainable management, especially for new plantations (Minocha et al, 2000). Plants possess complex defense mechanisms against phytopathogens (Sarwar et al, 2022a) that lead to biochemical and physiological alterations in plants.…”

Section: Introductionmentioning

confidence: 99%

Identification of resistance gene analogs of the NBS-LRR family through transcriptome probing and in silico prediction of the expressome of Dalbergia sissoo under dieback disease stress

Ijaz

Haq²,

Khan³

et al. 2022

Front. Genet.

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Dalbergia sissoo is an important timber tree, and dieback disease poses a dire threat to it toward extinction. The genomic record of D. sissoo is not available yet on any database; that is why it is challenging to probe the genetic elements involved in stress resistance. Hence, we attempted to unlock the genetics involved in dieback resistance through probing the NBS-LRR family, linked with mostly disease resistance in plants. We analyzed the transcriptome of D. sissoo under dieback challenge through DOP-rtPCR analysis using degenerate primers from conserved regions of NBS domain-encoded gene sequences. The differentially expressed gene sequences were sequenced and in silico characterized for predicting the expressome that contributes resistance to D. sissoo against dieback. The molecular and bioinformatic analyses predicted the presence of motifs including ATP/GTP-binding site motif A (P-loop NTPase domain), GLPL domain, casein kinase II phosphorylation site, and N-myristoylation site that are the attributes of proteins encoded by disease resistance genes. The physicochemical characteristics of identified resistance gene analogs, subcellular localization, predicted protein fingerprints, in silico functional annotation, and predicted protein structure proved their role in disease and stress resistance.

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Section: Introductionmentioning

confidence: 99%

Identification of resistance gene analogs of the NBS-LRR family through transcriptome probing and in silico prediction of the expressome of Dalbergia sissoo under dieback disease stress

Ijaz

Haq²,

Khan³

et al. 2022

Front. Genet.

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“…Biotic and abiotic factors also affect trees that result in declined tree populations (Rehman et al, 2012). Development of resistant material against various natural calamities will reduce supply-demand strain and will be more beneficial for farmers as well for industries (Minocha and Jain, 2000). Different conventional and non conventional strategies have been employed to multiply the resistant plants in huge number (Pradhan et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Development of Source Independent Micropropagation System in <i>Dalbergia sissoo</i> Roxb, as a basis for Germplasm Conservation and Disease Free Plants Production

Zaidi¹,

Ijaz²,

Khan³

et al. 2016

mpb

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Plant micropropagation has becoming an imperative strategy at commercial level that provides large number of plants in less time and space. In woody trees, micropropagation is more tedious and cumbersome process than other crop plants. Less success rate in tree micropropagation has been reported in literature. Therefore in this study, expedite, reproducible and source independent micropropagation protocol was developed in Dalbergia sissoo (Shisham) that is an economically important timer yielding tree with medicinal values. For this purpose, eight micropropagation media were studied with various combination of BAP (benzylaminopurine) and IAA (indole acetic acid). Auxially and epicormic shoots of Dalbergia sissoo having at least one nodal bud were used as explants. We studied the impact of these media combination and explant types on micropropagation. We found MPMS5 is the best medium combination that gave maximum number of shoots (2.78 shoots per explant) followed by MPMS7. This selected media combination can be used for Dalbergia sissoo micropropagation as a routine work. Similarly rooting media combinations were also studied for the best root induction in micropropagated shoots of Dalbergia sissoo but little success had been achieved.

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“…Plant regeneration by tissue culture, through either organogenesis or somatic embryogenesis, is a prerequisite for potential clone propagation, genetic transformation and in vitro preservation for germplasm from timber trees, including andiroba (Handley, 1995;Park et al, 1998;Minocha and Jain, 2000). Clone propagation in aseptic conditions is an alternative method of propagation for some medicinal plant species with large-scale production issues, accelerating the conventional propagation process and producing genetically identical plants (Zhou and Wu, 2006).…”

Section: Introductionmentioning

confidence: 99%

The effect of growth regulators on two different in vitro-cultured explants of Carapa guianensis

Oliveira¹,

Henriques²,

Macedo³

2015

J. Med. Plants Res.

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Carapa guianensisAubl. (Meliaceae), known locally as andiroba, is a multi-use species from Amazonia. Andiroba oil is considered an important natural product in the Brazilian market, and international demand is increasing due to its cosmetic and pharmaceutical potential. C. guianensis trees produce seed irregularly over different harvest periods, leading to inconsistent oil production and difficulties with supply. No management plans or protocols have been developed for in vitro or clonal production of Carapa seedlings and the maintenance of genetic resources. The objective of this study was to assess the effect of growth regulators on explants (young leaves, old leaves and apical buds). Explants consisting of leaf segments 1 cm on a side were cultivated in MS medium with and without growth regulators. Evaluation was based on fresh and dry weight of the explants after 20 days. In the media with 2,4-dichlorophenoxyacetic acid (5, 15, 35 or 45 µM), changes were observed in weight and explant appearance (callus). Bud breakage and development of shoots were achieved using 5 µM of 6benzylaminopurine. Overall, the results showed that 2,4-dichlorophenoxyacetic acid stimulates callus formation on andiroba foliar explants, while 6-benzylaminopurine was superior to thidiazuron for the initial development of shoots.

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Tissue Culture of Woody Plants and Its Relevance to Molecular Biology

Cited by 15 publications

References 158 publications

Identification of resistance gene analogs of the NBS-LRR family through transcriptome probing and in silico prediction of the expressome of Dalbergia sissoo under dieback disease stress

Identification of resistance gene analogs of the NBS-LRR family through transcriptome probing and in silico prediction of the expressome of Dalbergia sissoo under dieback disease stress

Development of Source Independent Micropropagation System in <i>Dalbergia sissoo</i> Roxb, as a basis for Germplasm Conservation and Disease Free Plants Production

The effect of growth regulators on two different in vitro-cultured explants of Carapa guianensis

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