Tissue Culture Techniques for Native Amazonian Fruit Trees

Pasqual, Moacir; Chagas, Edvan Alves; Soares, Joyce Dória Rodrigues; Rodrigues, Filipe Almendagna

doi:10.5772/52211

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…selection, its use in biotechnological processes, details regarding its ecological management and the establishment of commercial orchards/plant husbandries (Kinupp, 2011). Studies that focus on the in vitro propagation of Red Strawberry Guava can promote important mechanisms for the sustainable exploitation of this species; mechanisms that, up until now, have been rather scarce (Pasqual, Chagas, Soares, & Rodrigues, 2012).…”

Section: Introductionmentioning

confidence: 99%

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Freire¹,

Gardin²,

Baratto³

et al. 2016

JAS

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Red Araza, or Red Strawberry Guava (Psidium cattleianum Sabine) is a native Brazilian Atlantic Forest species of the Myrtaceae family, whose seeds exhibit integumental dormancy. Due to its importance to different industries worldwide, recent research efforts are seeking to expand this species' micropropagation processes using in vitro seedling germination, especially since in vitro micropropagation of adult plant material has, so far, been limited. This research effort evaluated different methods of overcoming integumental dormancy during in vitro germination of the Red Araza, so as to allow future micropropagation of the species. The seeds' emergence and vigor were evaluated based on mechanical and acid scarification, using different substrates and immersions in solutions with different levels of gibberellic acid (GA 3 ), and on the influence of the pre-immersion of seeds in water and sulfuric acid. The mechanical and acid scarification of the seeds, combined or separate, resulted in higher in vitro germination percentages and a higher germination rate index (GRI). Pre-immersion in distilled water (20 hours) also proved to be efficient for the germination of the Red Araza seed, with 76.2% of the seeds germinating and a higher speed of emergence (GRI = 0.18). When compared to a Murashige and Skoog (MS-zero) medium, sowing in a hydrophilic cotton substrate showed greater emergence and vigor, with approximately 70% of the seeds germinating. Treating the seeds by pre-immersing them in GA 3 turned out to be unnecessary. The methods used for overcoming integumental dormancy during in vitro germination of Red Araza seeds proved to be efficient, and could be used to develop micropropagation protocols of seminal origin for this species.

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Section: Introductionmentioning

confidence: 99%

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Freire¹,

Gardin²,

Baratto³

et al. 2016

JAS

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“…In the case of seedlings from São Paulo, which is from another state, the similarity with the seedlings from Lagoa da Confusão may be related to environmental factors and adaptability, as the seedlings were planted under the same conditions. Pasqual et al (2012) observed variability between the morphological characteristics in 16 genotypes of Bacuri (Platonia insignis Mart.) that compose the germplasm collection in the Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) Middle-North, and using the same method they reported the formation of five groups.…”

Section: Resultsmentioning

confidence: 95%

Morphological divergence of seedlings Calophyllum brasiliense cambes collected in São Paulo and Tocantins

Nascimento¹,

Silva²,

Reyes

et al. 2021

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Objective: The objective was to quantify the morphological diversity of Calophyllum brasiliense Cambes from four municipalities of the State of Tocantins employing morphological characteristics, to strengthen information on conservation and future breeding of the species. Methodology: Seeds were collected from four municipalities in the State of Tocantins and one in the State of São Paulo. The following were evaluated: plant height, stem diameter, root length, number of leaves, leaf area, root dry mass, shoot dry mass, total dry mass, and Dickson´s quality index. The data were subjected to univariate analysis of variance, Tocher grouping method, and UPGMA, obtaining a dendrogram through the generalized Mahalanobis distance. Results: The results showed a statistical difference of 1 and 5% probability. Dueré stood out in stem diameter(5.52 mm), Sandolandia in height (34.84 cm) and root length (42.13 cm). Formoso stood out in the number of leaves (34 leaves). Lagoa da Confusão in leaf area (856.28 cm 2 ) and São Paulo in root dry mass (16.20 g), shoot dry mass (12.38 g), total dry mass (16.20 g), and Dickson´s quality index (1.57). Implications: Variations in morphological characteristics can be used as a tool for genetic studies of guanandi progeny accordingto their similarity and/or differences. Conclusions: The morphological divergence evidenced that among the five studied areas it is possible to direct the collection of seeds to subsidize conservation strategies and future breeding of the species.

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“…A regeneração de plantas in vitro a partir de calos é um sistema bem utilizado para a micropropagação de plantas frutíferas. Para o cupuaçuzeiro, a embriogênese somática tem sido pouco utilizada, sendo a calogênese, a primeira etapa desse processo, cujos trabalhos na área carecem de avaliação de crescimento e comportamento dos calos (LEDO et al, 2002;PASQUAL et al, 2012;FERREIRA et al, 2013).…”

Section: Introductionunclassified

CALOGÊNESE in vitro EM EXPLANTES FLORAIS DE CUPUAÇU USANDO THIDIAZURON E ÁCIDO 2,4-DICLOROFENOXIACÉTICO

Ramos¹,

Rodrigues²,

Lemos³

et al. 2017

Enci Bio

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RESUMOPara dar suporte ao melhoramento genético do cupuaçuzeiro, a micropropagação é importante pela necessidade de obtenção de plantas genética e morfologicamente iguais e uniformes. A calogênese é uma etapa inicial da regeneração de plantas via embriogênese somática, pois a partir de calos embriogênicos há desenvolvimento de embriões. Visando induzir a calogênese in vitro em estruturas florais de cupuaçuzeiro, cógulas, estaminoides e ovários foram cultivados em meio de indução de calos, meio de cultura DKW, suplementado com thidiazuron (TDZ) (0,0025; 0,005; 0,015; 0,02; ou 0,01 mg l de BAP. O delineamento foi inteiramente casualizado em fatorial 3 X 6 (três tipos de explante e seis meios de cultura). Os explantes aos 28 dias de cultivo foram transferidos para um terceiro meio de cultura, constituído de sais e vitaminas DKW desprovido de reguladores de crescimento. A avaliação considerou o número de explantes calogênicos de cada tipo de explante nos meios de cultura em três estádios de desenvolvimento dos calos (pequeno, médio ou grande), conforme o diâmetro da massa calosa. Os dados foram analisados quanto ao percentual de calogênese e testes de comparação de média de Tukey. Todos os explantes, cógulas, estaminoides e ovários responderam à indução, ao crescimento e à multiplicação de calos. As cógulas e os estaminoides responderam em maior número na concentração de 0,0025 mg l -1de TDZ e 1 mg l -1 de 2,4-D no meio inicial, sendo a mais indicada para a calogênese usando explante floral de cupuaçuzeiro nas condições desse estudo. PALAVRAS-CHAVE: Cógulas; embriogênese somática; micropropagação. CALLOGENESIS in vitro IN CUPUASSU FLORAL EXPLANTS USINGTHIDIAZURON AND 2,4-DICHLOROPHENOXYACETIC ACID ABSTRACT To assist the development of commercial plantations of cupuassu, micropropagation studies are fundamental for the need to obtain genetically and morphologically plants

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Tissue Culture Techniques for Native Amazonian Fruit Trees

Cited by 5 publications

References 21 publications

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Morphological divergence of seedlings Calophyllum brasiliense cambes collected in São Paulo and Tocantins

CALOGÊNESE in vitro EM EXPLANTES FLORAIS DE CUPUAÇU USANDO THIDIAZURON E ÁCIDO 2,4-DICLOROFENOXIACÉTICO

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