Tissue cytogenetic studies in chronic ulcerative colitis and carcinoma of the colon

Xavier, Rogério Gastal; Prolla, João Carlos; Bemvenuti, Giovani A.; Kirsner, Joseph B.

doi:10.1002/1097-0142(197409)34:3<684::aid-cncr2820340327>3.0.co;2-p

Cited by 16 publications

(3 citation statements)

References 26 publications

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“…Unbanded chromosome studies in gastrointestinal cancers have been carried out on malignant effusions (Benedict and Porter, 1972;Ishihara et al, 1961 and1963;Ishihara and Sandberg, 1963;Makino et al, 1964) and later on the primary tumors (Atkin, 1974;Baker and Atkin, 1970; Enterline and Arvan, 1967;Lubs and Clark, 1963;Lubs and Kotler, 1967;Miles, 1967; Sandberg and Yamada, 1966;Xavier et al, 1974;Yamada et af., 1966). The numerical aberrations observed in colorectal cancers were separable into a near-diploid group (46-48 chromosomes) and a broad group ranging from the hypotriploid to the hypotetraploid levels (Atkin, 1976).…”

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confidence: 99%

Chromosomal banding patterns in human large bowel cancer

Reichmann

Martin

Levin

1981

Intl Journal of Cancer

121

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Cytogenetic banding studies were performed on direct preparations from 31 large bowel tumors. Twenty-eight of these were from primary sites and three from metastases. Some distinctive patterns were observed. The 31 tumors were separable into four groups: (1) Tumors with normal karyotypes (three cases); (2) tumors with simple gains of chromosomes (three cases); (3) tumors with gains, losses and structural aberrations (ten cases). Groups 1, 2 and 3 were all in the diploid range; and (4) tumors with hypotriploid-hypotetraploid karyotypes (15 cases). Non-random gains of several chromosomes, especially No. 8 and non-random losses of No. 17 were seen in this series. Thirteen cases had sex chromosome abnormalities. The most prominent structural abnormalities involved chromosomes No. 1 and No. 5. Marker chromosomes were sometimes absent, but when present ranged from simple translocations to the most complex rearrangements. Doubling of chromosome and/or marker chromosomes were prominent in Group 4. Double minutes were seen in all three metastatic tumors and three primary cancers. Premature chromosome condensation was observed in three cases in the hypotriploid-hypotetraploid group.

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confidence: 99%

Chromosomal banding patterns in human large bowel cancer

Reichmann

Martin

Levin

1981

Intl Journal of Cancer

121

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“…Occasionally, murine metaphase spreads were also observed, although these were infrequent. Tumour Lines AC4, GC3, VRC5 and ELC2 produced some obvious marker chromosomes, a feature characteristic of human colorectal tumours (Xavier et al, 1974). Two marker chromosomes present in later passages of ELC2 were also present on Passage 2 from female hosts.…”

Section: Retention Of Histological Integritymentioning

confidence: 96%

Maintenance of biological and biochemical characteristics of human colorectal tumours during serial passage in immune-deprived mice

Houghton¹,

Taylor²

1978

Br J Cancer

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The effect of serial passage in immune-deprived mice on certain biological and biochemical parameters has been studied in a series of 6 human colorectal tumour xenografts. Histological integrity is maintained for up to 10 serial passages, together with production of epithelial mucins and carcinoembryonic antigen. Passaged tumours retain human lactate dehydrogenase and glucose-6-phosphate dehydrogenase isoenzyme patterns and a human chromosome constitution. The induction of a murine tumour has been identified in this system, and the importance of routine checks for the presence of human tissue during serial passage is stressed. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4

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“…The technique presently used is similar to that recommended by Xavier et al (1974). All samples must be taken within 15 min of the surgical specimen being freed and must be taken from the inner face of the peripheral tumoural swelling.…”

Section: Discussionmentioning

confidence: 99%

Human chromosome analysis in 24 cases of primary carcinoma of the large intestine: contribution of the G-banding technique

Couturier-Turpin¹,

Couturier²,

Nepveux³

et al. 1982

Br J Cancer

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As in the haemopathies, the application of cytogenetics to epithelial cancers could aid in the study of their pathogenesis evaluation. In this context we performed chromosome analyses on a series of human colo-rectal cancers. The technique was consistently reliable since the modal number of chromosomes could be determined in all 24 cases. In 22, karyotypes could also be established. Each tumour was characterized by a single cell clone in 21 cases and by a mosaic of 2 populations in 3 cases. Numerical anomalies were not due to chance: they enabled near-diploid (11 cases), near-triploid (9 cases), mosaic (3 cases) and highly polyploid (1 case) cancers to be distinguished. Supernumerary chromosomes were primarily in groups C and F. The most frequent markers before denaturation techniques were No 2q +, No F and minutes. Each time double-minutes were observed (5 cases), they were in invasive cancers (B and C Dukes classification). Cells were generally diploid in non-invasive cancers with fewer quantitative and structural anomalies. Tumour cytogenetics were related to the histological type and localization in the colon, as well as to the local and metastatic spread. Images Fig. 2 Fig. 3 Fig. 4

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Tissue cytogenetic studies in chronic ulcerative colitis and carcinoma of the colon

Cited by 16 publications

References 26 publications

Chromosomal banding patterns in human large bowel cancer

Chromosomal banding patterns in human large bowel cancer

Maintenance of biological and biochemical characteristics of human colorectal tumours during serial passage in immune-deprived mice

Human chromosome analysis in 24 cases of primary carcinoma of the large intestine: contribution of the G-banding technique

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