2013
DOI: 10.1016/j.biomaterials.2013.04.058
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Tissue decellularization by activation of programmed cell death

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Cited by 71 publications
(77 citation statements)
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“…Decellularization of tissue engineered grafts represents a promising strategy to accelerate the translation of such therapies into the clinic by providing 'off-the-shelf' constructs that can facilitate endogenous tissue or organ regeneration [34][35][36]. The results of this study provide support for the hypothesis that a porous scaffold generated from hypertrophic cartilage ECM, engineered using allogeneic MSCs, will support vascularization and host-mediated bone formation upon implantation.…”
Section: Discussionsupporting
confidence: 52%
“…Decellularization of tissue engineered grafts represents a promising strategy to accelerate the translation of such therapies into the clinic by providing 'off-the-shelf' constructs that can facilitate endogenous tissue or organ regeneration [34][35][36]. The results of this study provide support for the hypothesis that a porous scaffold generated from hypertrophic cartilage ECM, engineered using allogeneic MSCs, will support vascularization and host-mediated bone formation upon implantation.…”
Section: Discussionsupporting
confidence: 52%
“…The multitude of parameters involved may also require the use of miniaturized high-throughput devices (e.g. microfluidic devices) followed by combinatorial analyses in order to define the minimal set of necessary eECM signals which are required for predictable instruction of bone regeneration [84].…”
Section: Discussionmentioning
confidence: 99%
“…Existing strategies rely on physical, chemical or enzymatic treatment that fail to achieve an efficient cell removal while preserving the bioactive ECM elements. A valuable direction may be represented by the specific activation of the apoptotic cell program [84], to selectively target the killing of the cellular fraction while reducing the side effects of ECM damage.…”
Section: Standardization Of Eecmmentioning
confidence: 99%
“…S2), which was previously proposed to target the living cell fraction with minimal changes in the ECM components (18). Primary hMSCs were thus retrovirally transduced with a death system based on the inducible dimerization of modified caspase 9 (iDS; Fig.…”
Section: Generation Of Hypertrophic Constructs Using Death-inducible mentioning
confidence: 99%
“…This implies the use of a devitalization strategy reducing alterations in the composition and architecture of the generated template to mimic both the physiologic regenerative milieu and the 3D structure of the fracture callus. Toward this objective, a devitalization approach has been proposed via the induction of apoptosis (18). In particular, an inducible genetic system (19) can be incorporated into primary hMSCs to specifically induce their apoptosis on exposure to a clinical-grade chemical compound.…”
mentioning
confidence: 99%