2018
DOI: 10.1038/s41598-018-31469-4
|View full text |Cite
|
Sign up to set email alerts
|

Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy

Abstract: The Raman spectral features from spinal cord tissue sections of transgenic, ALS model mice and non-transgenic mice were compared using 457 nm excitation line, profiting from the favourable signal intensity obtained in the molecular fingerprint region at this wavelength. Transverse sections from four SOD1G93A mice at 75 days and from two at 90 days after birth were analysed and compared with sections of similarly aged control mice. The spectra acquired within the grey matter of tissue sections from the diseased… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
7
0

Year Published

2020
2020
2022
2022

Publication Types

Select...
6
1
1

Relationship

0
8

Authors

Journals

citations
Cited by 10 publications
(7 citation statements)
references
References 29 publications
0
7
0
Order By: Relevance
“… 15 19 Pertinent to the present study, a recent report used stimulated Raman scattering microscopy in vivo to study nerve pathology in several mouse models of ALS. 20 In addition, spontaneous Raman has been used to study the spinal cord in the SOD1 G93A model 21 and to examine fly models of human muscle diseases (myopathies). 22 While these studies require either technically challenging in vivo methods or rely on ex vivo analysis, they demonstrate the potential of the technique for the study of neuromuscular disorders.…”
Section: Introductionmentioning
confidence: 99%
“… 15 19 Pertinent to the present study, a recent report used stimulated Raman scattering microscopy in vivo to study nerve pathology in several mouse models of ALS. 20 In addition, spontaneous Raman has been used to study the spinal cord in the SOD1 G93A model 21 and to examine fly models of human muscle diseases (myopathies). 22 While these studies require either technically challenging in vivo methods or rely on ex vivo analysis, they demonstrate the potential of the technique for the study of neuromuscular disorders.…”
Section: Introductionmentioning
confidence: 99%
“…Stimulated Raman scattering (SRS) microscopy-based methods are a set of powerful label-free techniques, which have been successfully applied to detect peripheral nerve degeneration and tissue degeneration of the spinal cord in mouse models of amyotrophic lateral sclerosis (ALS) 283 , 284 . They were also used in the quantification and spectral analysis of native polyQ aggregates with subcellular resolution in live cells 285 .…”
Section: Building a C-mod Discovery Platformmentioning
confidence: 99%
“…Interestingly, the expression of TEs was higher in the dorsal and ventral horns, than the expression observed in more medial or distal regions of the spinal cord (Figure 1, right panels). This is an interesting finding because the cell population most affected during ALS is located within the ventral horn [16]. Then, we analyzed the TE expression according to the categories of LINE, SINE, LTR, and DNA transposons (Figure 2).…”
Section: Spatially Resolved Te Expression Of P120 Spinal Cord Section...mentioning
confidence: 99%