Tissue distribution and change in potato starch phosphorylase mRNA levels in wounded tissue and sprouting tubers

Bhatt, Anuj M.; Knowler, John T.

doi:10.1111/j.1432-1033.1992.tb16718.x

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…These genes also have a similar pattern of expression in the organs of potato plants: a low level of expression in most organs and a high level of expression in sink tubers (Mfiller-R6ber et al 1990;Kogmann et al 1991;Bhatt and Knowler 1992). These genes also have a similar pattern of expression in the organs of potato plants: a low level of expression in most organs and a high level of expression in sink tubers (Mfiller-R6ber et al 1990;Kogmann et al 1991;Bhatt and Knowler 1992).…”

Section: Accumulation Of Starch Phosphorylase M R N a Is Induced By Tmentioning

confidence: 99%

Induction of the plastidic starch-phosphorylase gene in potato storage sink tissue

St-Pierre

Brisson

1995

Planta

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The expression of the gene encoding the plastidic enzyme starch phosphorylase (EC 2.4.1.1) varies according to tissue carbohydrate status. Incubation of excised potato (Solanum tuberosum L. cv. D6sir6e) leaves carrying a portion of the stem under a short photoperiod resulted in a drastic accumulation of starch, accompanied by a rapid increase in the level of phosphorylase mRNA and by a similar change in phosphorylase protein level. However, under the same incubation conditions, the transcriptional activity of the phosphorylase promoter in transgenic plants did not change markedly. Therefore, the increased expression of the phosphorylase gene in petioles of stem cuttings is not controlled by the level of initiation of transcription. Phosphorylase mRNA accumulated to a high level in petioles of detached leaves kept under constant light for 24 h, but not in petioles kept in the dark. The effect of light on the accumulation of the mRNA was appreciably reduced if the petioles were incubated in ethylendiaminetetraacetic acid (ED-TA), a treatment known to increase phloem exudation in detached leaves. The inhibition by EDTA could be partially counteracted by the addition of sucrose to the incubation solution. Furthermore, incubation of petioles in darkness in solutions with high levels of sucrose resulted in enhanced expression of the gene. These results suggest that sucrose, the main compound transported by phloem in potato, is involved in the regulation of the starch phosphorylase gene. This also indicates that conditions favouring starch synthesis lead to increased expression of the phosphorylase gene.

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Section: Accumulation Of Starch Phosphorylase M R N a Is Induced By Tmentioning

confidence: 99%

Induction of the plastidic starch-phosphorylase gene in potato storage sink tissue

St-Pierre

Brisson

1995

Planta

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Actin Depolymerization Affects Stress-Induced Translational Activity of Potato Tuber Tissue1

Morelli

Zhou

et al. 1998

Plant Physiology

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Changes in polymerized actin during stress conditions were correlated with potato (Solanum tuberosum L.) tuber protein synthesis. Fluorescence microscopy and immunoblot analyses indicated that filamentous actin was nearly undetectable in mature, quiescent aerobic tubers. Mechanical wounding of postharvest tubers resulted in a localized increase of polymerized actin, and microfilament bundles were visible in cells of the wounded periderm within 12 h after wounding. During this same period translational activity increased 8-fold. By contrast, low-oxygen stress caused rapid reduction of polymerized actin coincident with acute inhibition of protein synthesis. Treatment of aerobic tubers with cytochalasin D, an agent that disrupts actin filaments, reduced wound-induced protein synthesis in vivo. This effect was not observed when colchicine, an agent that depolymerizes microtubules, was used. Neither of these drugs had a significant effect in vitro on run-off translation of isolated polysomes. However, cytochalasin D did reduce translational competence in vitro of a crude cellular fraction containing both polysomes and cytoskeletal elements. These results demonstrate the dependence of wound-induced protein synthesis on the integrity of microfilaments and suggest that the dynamics of the actin cytoskeleton may affect translational activity during stress conditions.

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Tissue distribution and change in potato starch phosphorylase mRNA levels in wounded tissue and sprouting tubers

Cited by 2 publications

References 29 publications

Induction of the plastidic starch-phosphorylase gene in potato storage sink tissue

Induction of the plastidic starch-phosphorylase gene in potato storage sink tissue

Actin Depolymerization Affects Stress-Induced Translational Activity of Potato Tuber Tissue1

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