Tissue distribution and cytofluorometric analysis of oral mucosal T cells in the balb/c mouse

Bolduc, Carl; Waterfield, J D; Deslauriers, N

doi:10.1016/0923-2494(90)90016-r

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…In mammals, the normal salivary glands host an indigenous population of immunocytes that can take the form of organized lymphoid tissue in both minor (4,22,23) and major (24) salivary glands in association with the duct system. Although lymphoid aggregations have not been reported in rodents, T and B cells and macrophages can be recovered from major salivary glands after enzymatic digestion (5,25), and the oral mucosa and its minor salivary gland network host populations of T and B lymphocytes and mast cells (19,(26)(27)(28). However, the strong preference of immunocytes for the vicinity of ducts and blood vessels argues in favour of an organized, although diffuse, immune system in mice.…”

Section: Discussionmentioning

confidence: 99%

Murine autoimmune exocrinopathy: the minor salivary gland network shows a dichotomous pattern of histopathologic involvement

Barbeau

Deslauriers

1995

J Oral Pathology Medicine

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The minor salivary gland network of the MRL/l mouse was investigated in a kinetic study and compared with the major submandibular gland. We report that minor salivary glands adopt two mutually exclusive patterns of inflammatory lesions depending on the gland. The first pattern is characteristic of human Sjogren's syndrome. It developed during the second month, affected 89% of the animals over 20 weeks old, and consisted of an accumulation of mononuclear cells around the duct system. Only the anterior buccal gland (ABG) showed this pattern, which is shared by the major salivary glands. The ratio of CD4+ to CD8+ cells was the same in lesions and in healthy tissue. No neutrophils were found in these lesions. The second pattern affected all the minor salivary glands except the ABG. These lesions were never observed before the age of 20 weeks and affected 38% of MRL/l mice between the ages of 10-32 weeks. In this pattern, neutrophils were frequently found, but mainly gathered at the periphery of the gland lobules. That a systemic immunoregulatory defect may be expressed as two different patterns of histopathology in the minor salivary glands suggest that the network behaves as a dichotomous entity depending on particular microenvironmental influences.

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Section: Discussionmentioning

confidence: 99%

Murine autoimmune exocrinopathy: the minor salivary gland network shows a dichotomous pattern of histopathologic involvement

Barbeau

Deslauriers

1995

J Oral Pathology Medicine

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“…Due to coating antibodies, a faint background was observed but positive cells could easily be discriminated over the background because of the high concentration of the specific labelled antibody at the cell surface. The ratio of L3T4+/Lyt-2.2+ cells found in the spleen (3,14) and the oral mucosa (3) was conserved among panned cells since L3T4+ panned cells were approximately twice as numerous as Lyt-2.2+ panned cells.…”

Section: Bolduc and Deslauriersmentioning

confidence: 96%

“…It contains a population of B lymphocytes associated with the minor salivary gland network (MSG-network) (I), macrophages (2) and a recently described population of Thy-1.2+ cells that is well compartmentalized in the oral epithelium and in the minor salivary glands (3). The majority of Thy-1.2+ cells either express the L3T4 or the Lyt-2.2 marker and are 2-3 times more numerous than B cells or macrophages (1,3). According to their incidence and distribution we hypothesized that they may play a part in local immune responses either as effector cells or as regulatory cells.…”

Section: Introductionmentioning

confidence: 99%

“…The entire oral mucosa, which includes cheeks and hard and soft palates, was excised free of the underlying muscle tissue immediately after killing by exsanguination. Tissues were then digested with collagenase (Type I, Sigma, Chemical Co, St.Louis, MO, USA) and hyaluronidase (Type IV-S, Sigma) in complete Iscove's medium (Sigma) containing 100U/ml penicillin, 1 OOpg/ml streptomycin, 50 pg/ml gentamicin (Sigma) and 5% fetal calf serum (FCS, Sigma), as described previously (3). In the resulting single cell suspensions, the nucleated cell yield was approximately 1 -2xlO4 cells/mg tissue (about 1x106 nucleated cells/mucosa).…”

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confidence: 99%

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Receptors for the F_CPortion of Immunoglobulins (F_CR) on Murine Oral Mucosal T Cells

Bolduc¹,

Deslauriers²

1990

Immunological Investigations

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The incidence and distribution of immunoglobulin receptors on oral mucosal T cells were investigated. Enrichment of either Thy-1.2, L3T4 or Lyt-2.2-positive cells was achieved by the use of a panning procedure following cell extraction by an optimized digestion method that preserved all three T-cell markers. The percentage of cells possessing Fc receptors was determined by using immunoglobulin-coated (IgM, IgG or IgA) fluorescent microspheres in a multipoint rosetting assay. We report that approximately 60% of Thy-1.2, L3T4 and Lyt-2.2 positive cells bear Fc gamma R whereas less than 5% of T cells of any subset bear Fc alpha R or Fc mu R. In frozen tissue sections, Fc gamma R+ cells were mainly scattered throughout the basal and suprabasal epithelium and were observed at a lower frequency in the minor salivary gland network. From their distribution, it is anticipated that Fc gamma R+ cells may be involved in the surveillance of the epithelium while the minor Fc alpha R+ L3T4+ T lymphocyte population may promote the expression of sIgA by resident sIgM-bearing B cells, and their differentiation into IgA plasma cells.

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Peroral Immunization with a Cholera Toxin-Linked Bacterial Protein Antigen and Synthetic Peptide

Mw¹,

Hy²,

Pl³

et al. 1992

Genetically Engineered Vaccines

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Tissue distribution and cytofluorometric analysis of oral mucosal T cells in the balb/c mouse

Cited by 10 publications

References 37 publications

Murine autoimmune exocrinopathy: the minor salivary gland network shows a dichotomous pattern of histopathologic involvement

Murine autoimmune exocrinopathy: the minor salivary gland network shows a dichotomous pattern of histopathologic involvement

Receptors for the F_CPortion of Immunoglobulins (F_CR) on Murine Oral Mucosal T Cells

Peroral Immunization with a Cholera Toxin-Linked Bacterial Protein Antigen and Synthetic Peptide

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Tissue distribution and cytofluorometric analysis of oral mucosal T cells in the balb/c mouse

Cited by 10 publications

References 37 publications

Murine autoimmune exocrinopathy: the minor salivary gland network shows a dichotomous pattern of histopathologic involvement

Murine autoimmune exocrinopathy: the minor salivary gland network shows a dichotomous pattern of histopathologic involvement

Receptors for the FCPortion of Immunoglobulins (FCR) on Murine Oral Mucosal T Cells

Peroral Immunization with a Cholera Toxin-Linked Bacterial Protein Antigen and Synthetic Peptide

Contact Info

Product

Resources

About

Receptors for the F_CPortion of Immunoglobulins (F_CR) on Murine Oral Mucosal T Cells