Tissue distribution and duration of mouse hepatitis virus in naturally infected immunocompetent ICR (CD-1) and immunodeficient athymic nude-nu mouse strains used for ovarian transplantation and in vitro fertilization

Abstract

Mouse hepatitis virus (MHV) is a ribonucleic acid (RNA) virus belonging to the Coronaviridae family and is one of the most prevalent viruses found in laboratory mice throughout the world (Barthold &

Risk Assessment of Mouse Hepatitis Virus Infection via In Vitro Fertilization and Embryo Transfer by the Use of Zona-Intact and Laser-Microdissected Oocytes1

“…Several studies used embryos produced in vivo or in vitro from mice that harbored different viral or bacterial pathogens, including Sendai virus, MHV, mouse parvovirus (MPV), and Pasteurella pneumotropica [4,7,33,34,36,42]. The embryos were washed using different washing procedures and transferred to pseudopregnant recipients.…”

Helicobacter typhlonius was detected in the sex organs of three mouse strains but did not transmit vertically

“…Previous findings (Scavizzi & Raspa 2004) on mouse hepatitis virus (MHV) indicated that at least ovary transplantation could transmit this virus. In contrast, in the case of H. typhlonius, the pathogen was never detected in nude-nu (nu/nu and nu/ þ ) recipient females after IVF, ET or infected ovary transplantation, and never in the resulting offspring.…”

Rodent and Germplasm Trafficking: Risks of Microbial Contamination in a High-Tech Biomedical World

“…Retroviral contamination of donor tissue by a recipient female has occurred with transmission to recovered offspring (Lock et al 1988), demonstrating that genetic alteration via retroviruses can occur in offspring as a consequence of either the donor or recipient female. Ovarian tissue from mice naturally infected with MHV has been shown to transmit MHV to the recipient females and offspring (Scavizzi and Raspa 2004). In contrast, H. typhlonius-infected ovarian tissue transplantation did not result in infection of the recipient female or offspring (Scavizzi and Raspa 2006).…”