Tissue Distribution of C14 After the Intravenous Injection of Labeled Chylomicrons and Unesterified Fatty Acids in the Rat

Bragdon, Joseph H.; Gordon, Robert

doi:10.1172/jci103640

Cited by 359 publications

(94 citation statements)

References 9 publications

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“…In most tissues there was little or no difference. The main difference was in adipose tissue, and only in the fed state as previously observed by Bragdon & Gordon (1958). Total uptake in adipose tissue accounted for 20-30 % of the injected chylomicron label and this amount was about the same as the amount of fatty acids that apparently do not enter the plasma FFA.…”

supporting

confidence: 51%

Lipoprotein lipase: regulation and role in lipoprotein metabolism

et al. 1997

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supporting

confidence: 51%

Lipoprotein lipase: regulation and role in lipoprotein metabolism

et al. 1997

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“…2). The relationship with respect to visceral adipose tissue remained significant excluding the results from four participants whose omental biopsies were collected at 6, 7, 8, or 11 h after the [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]oleate bolus. In contrast, there was no significant relationship between femoral lipid SA and LBSQ fat mass (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In vivo animal studies have shown that circulating FFA can be taken up and restored in subcutaneous adipose tissue, thus forming a cycle of release and reuptake (5)(6)(7)(8)(9)(10)(11). By using a combination of FFA tracers and tissue biopsies, it has been possible to assess total uptake (storage and oxidation) or, exclusively, storage of circulating FFA in adipose tissue.…”

mentioning

confidence: 99%

Plasma Free Fatty Acid Storage in Subcutaneous and Visceral Adipose Tissue in Postabsorptive Women

et al. 2008

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1OBJECTIVE-We assessed the direct (VLDL-triglycerides [VLDL-TG] independent) storage of circulating free fatty acids (FFAs) in visceral and subcutaneous fat in postabsorptive women.RESEARCH DESIGN AND METHODS-Twelve women (BMI 29.6 Ϯ 6.6 kg/m 2 ) received an identical, intravenous bolus dose of [1-14 C]oleate followed by timed subcutaneous fat biopsies (abdominal and femoral) and then omental fat biopsy during tubal ligation surgery. Regional fat masses were assessed by combining dual-energy X-ray absorptiometry and computed tomography scanning. Separately, we assessed the fraction of FFA tracer entering VLDL-TG over the time representing the delay in collecting omental fat.RESULTS-Site-specific fat specific activity (SA) (dpm/g lipid) decreased as a function of fat mass in both upper-body subcutaneous (UBSQ) and visceral fat depots. These patterns are consistent with dilution of a relatively fixed amount of FFA tracer within progressively greater amounts of fat. Interestingly, femoral SA did not vary as a function of lower-body subcutaneous (LBSQ) fat mass. [1-14 C]oleate storage per million LBSQ adipocytes was positively associated with LBSQ fat mass, but no significant relationships were observed in UBSQ or visceral fat depot. The fraction of [1-14 C]oleate stored in UBSQ, LBSQ, and visceral fat was 6.7 Ϯ 3.2, 4.9 Ϯ 3.4, and 1.0 Ϯ 0.3%, respectively. Only ϳ4% of the tracer traversed VLDL-TG over 9.5 h. CONCLUSIONS-The increase in FFA tracer storage per adipocyte as a function of LBSQ fat mass implies that LBSQ adipocytes, in contrast to UBSQ and omental adipocytes, store more FFA in women with greater adiposity. The direct FFA storage pathway might play a role in favoring lower-body fat accumulation in women.

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“…Recent studies by Bragdon and Gordon (23) have shown that fasting produced profound alteration in the tissue distribution of C14-labeled chylomicron triglyceride. i\Iore of the label was found in the myocardium, and considerably less in the adipose tissue of the fasted animals.…”

Section: Discussionmentioning

confidence: 99%