Tissue distribution of monoamine neurotransmitters in normal and regenerating arms of the feather star Antedon mediterranea

Carnevali, M. Daniela Candia; Bonasoro, F.; Invernizzi, Roberto William; Lucca, Elisa; Welsch, U.; Thorndyke, Michael C.

doi:10.1007/s004410050651

Cited by 23 publications

(43 citation statements)

References 27 publications

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“…The regenerate was three times longer for the arm cut at 50·mm from the tip but less differentiated (DI of 45% versus 70%) than the one cut at 5·mm from the tip. Bonasoro, 1994;Candia Carnevali et al, 1995;Candia Carnevali et al, 1996;Candia Carnevali et al, 1997;Candia Carnevali et al, 1998). If neurally secreted factors are responsible for our observed differences in growth versus differentiation, it is possible to hypothesize that growth and/or differentiation rates could be proportional to the concentration and/or identity of one or several of these factors.…”

Section: Origin Of the Variabilitymentioning

confidence: 93%

Growth or differentiation? Adaptive regeneration in the brittlestarAmphiura filiformis

Dupont¹,

Thorndyke²

2006

Journal of Experimental Biology

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SUMMARY Amphiura filiformis is a burrowing brittlestar, which extends arms in the water column when suspension feeding. In previous studies, unexpectedly high variability was observed in regeneration rate between individuals even when experiments were performed under identical conditions. The aims of this work were to understand this variability and interpret the observed variability in terms of adaptation to sublethal predation. Our experiments on the dynamics of arm regeneration in A. filiformis revealed that the developmental program during regeneration is well adapted to its burrowing life style. We demonstrate that there is a trade-off between regeneration in length and functional recovery for feeding (differentiation index). The amount of tissue lost (length lost), which represents the quantity of tissue needed to completely regenerate an intact arm with no previous history of regeneration, determines whether the arm will invest more energy in growth and/or in differentiation, which must be a reflection of the ability to differentially regulate developmental programs during regeneration. We show that combining regeneration rate with differentiation index provides an ideal tool for the definition of a standard temporal framework for both field and laboratory studies of regeneration.

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Section: Origin Of the Variabilitymentioning

confidence: 93%

Growth or differentiation? Adaptive regeneration in the brittlestarAmphiura filiformis

Dupont¹,

Thorndyke²

2006

Journal of Experimental Biology

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“…1A) were collected by scuba divers from the Tirrenian coast of Italy (Elba island) and maintained at [15][16] • C in a closed artificial sea-water system with a diet of invertebrate food for fine filter feeders (Coral Fliud -Marin). The visceral mass of each individual was removed from the calyx by gently pulling it after a superficial incision of the tegmen at the point of the arm branchings.…”

Section: Methodsmentioning

confidence: 99%

“…They are able to completely regenerate arms [2,[11][12][13][14][15][16][17][18][19][20][21], pinnules, cirri, and also viscera, including the whole gut, after self-induced or traumatic mutilation [1,2].…”

Section: Introductionmentioning

confidence: 99%

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

et al. 2006

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Abstract:Crinoids are able to regenerate completely many body parts, namely arms, pinnules, cirri, and also viscera, including the whole gut, lost after self-induced or traumatic mutilations. In contrast to the regenerative processes related to external appendages, those related to internal organs have been poorly investigated. In order to provide a comprehensive view of these processes, and of their main events, timing and mechanisms, the present work is exploring visceral regeneration in the feather star Antedon meditteranea. The histological and cellular aspects of visceral regeneration were monitored at predetermined times (from 24 hours to 3 weeks post evisceration) using microscopy and immunocytochemistry. The overall regeneration process can be divided into three main phases, leading in 3 weeks to the reconstruction of a complete functional gut. After a brief wound healing phase, new tissues and organs develop as a result of extensive cell migration and transdifferentiation. The cells involved in these processes are mainly coelothelial cells, which after trans-differentiating into progenitor cells form clusters of enterocytic precursors. The advanced phase is then characterized by the growth and differentiation of the gut rudiment. In general, our results confirm the striking potential for repair (wound healing) and regeneration displayed by crinoids at the organ, tissue and cellular levels.

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“…Blocking access of the nerve to the wound site prevented the di¡erentiation of the terminal structures and mitosis was reduced, although the epidermis still formed and cells gathered at the amputation zone. This in£uence of the nerve, possibly on di¡erentiation, may be mediated by a neurotransmitter, growth factor, a neuropeptide such as S1, or a combination of such molecules (Candia-Carnevali et al 1996). It is now known that the neural tissue plays a role in many regenerative processes and its investigation has become a fundamental underlying feature of regeneration studies (Singer & Geraudie 1989).…”

Section: Discussionmentioning

confidence: 99%

Patterns of bromodeoxyuridine incorporation and neuropeptide immunoreactivity during arm regeneration in the starfish Asterias rubens

Moss

Hunter

Thorndyke

1998

Phil. Trans. R. Soc. Lond. B

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Regeneration of the arm of the star¢sh, Asterias rubens (L.) (Echinodermata: Asteroidea) was examined using two preparations. The ¢rst involved regeneration of the entire arm tip and its associated sensory structures and the second examined regeneration of a small section of radial nerve cord in the mid-arm region. Cell cycle activity was investigated by incorporation of the thymidine analogue, bromodeoxyuridine (BrdU). Details of neuroanatomy were obtained by immunocytochemistry (ICC) using an antiserum to the recently isolated star¢sh neuropeptide, GFNSALMFamide (S1). BrdU labelling indicated that initial events occur by morphallaxis, with cell cycle activity ¢rst apparent after formation of a wound epidermis. As regeneration proceeded, BrdU immunoreactive (IR) nuclei revealed cell cycle activity in cells at the distal ends of the radial nerve cord epidermis, in the coelomic epithelium, the perihaemal and water vascular canal epithelia, and in the forming tube feet of both preparations. By varying the time between BrdU pulses and tissue ¢xation, the possible migration or di¡erentiation of labelled cells was investigated. Neuropeptide ICC indicated the extension of S1-IR nerve ¢bres into the regenerating area, soon after initial wound healing processes were complete. These ¢bres were varicose and disorganized in appearance, when compared to the normal pattern of S1-IR in the radial nerve. S1-IR was also observed in cell bodies, which reappeared in the reforming optic cushion and radial nerve at later stages of regeneration. Double labelling studies with anti-BrdU and anti-S1 showed no co-localization in these cell bodies, in all the stages examined. It appeared that S1-IR cells were not undergoing, and had not recently undergone, cell cycle activity. It cannot be con¢rmed whether S1-IR neurons were derived from proliferating cells of epithelial origin, or from transdi¡erentiation of epithelial cells, although the former mechanism is suggested. Di¡er-entiation of the regenerating structures to replace cells such as S1-containing neurons, is thought to involve cell cycle activity and di¡erentiation of epithelial cells in the epidermal tissue, possibly in association with certain types of coelomocytes which move into the regenerating area.

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Tissue distribution of monoamine neurotransmitters in normal and regenerating arms of the feather star Antedon mediterranea

Cited by 23 publications

References 27 publications

Growth or differentiation? Adaptive regeneration in the brittlestarAmphiura filiformis

Growth or differentiation? Adaptive regeneration in the brittlestarAmphiura filiformis

Visceral regeneration in the crinoid Antedon mediterranea: basic mechanisms, tissues and cells involved in gut regrowth

Patterns of bromodeoxyuridine incorporation and neuropeptide immunoreactivity during arm regeneration in the starfish Asterias rubens

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