Tissue distribution of stem cell factor in adults

Foster, Brittni M.; Langsten, Kendall; Mansour, Ammar; Shi, Lihong; Kerr, Bethany A.

doi:10.1016/j.yexmp.2021.104678

Cited by 5 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 21 Heterozygous germ line Kitl knockout mice display a reduction in HSC numbers 7 and a corresponding gene dosage effect on the systemic sKITL levels. 21 Because KITL is expressed by ECs outside and throughout the BM, 22 , 23 , 24 this raised the alternative possibility that EC-derived KITL might sustain HSCs primarily through sKITL, potentially produced outside the HSC niche or even outside the BM, rather than through the proposed endothelial niche cell expression of mKITL. 5 , 7 Herein, we pursued experiments to provide further insights into the regulatory roles of EC-derived systemic sKITL and EC niche–presented mKITL.…”

Section: Introductionmentioning

confidence: 99%

Loss of endothelial membrane KIT ligand affects systemic KIT ligand levels but not bone marrow hematopoietic stem cells

Matsuoka

Facchini

Luís

et al. 2023

Blood

View full text Add to dashboard Cite

A critical regulatory role of hematopoietic stem cell vascular niches in the bone marrow has been implicated to occur through endothelial niche cell expression of KIT Ligand. However, endothelial-derived KIT Ligand is expressed in both a soluble and membrane-bound form, and not unique to bone marrow niches and is also systemically distributed through the circulatory system. Here we confirm that upon deletion of both the soluble and membrane-bound form of endothelial-derived KIT Ligand hematopoietic stem cells are reduced in mouse bone marrow. However, deletion of endothelial-derived KIT Ligand was also accompanied by reduced soluble KIT Ligand levels in blood, precluding any conclusion as to whether the reduction in HSC numbers reflect reduced endothelial expression of KIT Ligand within HSC niches, elsewhere in the bone marrow and/or systemic sKIT Ligand produced by endothelial cells outside of the bone marrow. Notably, endothelial deletion specifically of the membrane bound form of KIT Ligand also reduced systemic levels of soluble KIT Ligand although with no effect on stem cell numbers, implicating a hematopoietic stem cell regulatory role primarily of soluble rather than membrane KIT Ligand expression in endothelial cells. In support of a role of systemic rather than local niche expression of soluble KIT Ligand, hematopoietic stem cells were unaffected in bones with deletion of KIT ligand when implanted in mice with normal systemic levels of soluble KIT Ligand. Our findings highlight the need for more specific tools to unravel niche-specific roles of regulatory cues expressed in hematopoietic niche cells in the bone marrow.

show abstract

Section: Introductionmentioning

confidence: 99%

Loss of endothelial membrane KIT ligand affects systemic KIT ligand levels but not bone marrow hematopoietic stem cells

Matsuoka

Facchini

Luís

et al. 2023

Blood

View full text Add to dashboard Cite

show abstract

“…IHC analysis on liver and intestine tissue was performed as previously reported (Foster et al., 2021). The primary antibody utilised was TLR4 (Catalogue no.…”

Section: Methodsmentioning

confidence: 99%

Characterisation of LPS+ bacterial extracellular vesicles along the gut‐hepatic portal vein‐liver axis

Jain,

Kumar,

Almousa

et al. 2024

J of Extracellular Vesicle

Self Cite

View full text Add to dashboard Cite

Gut microbiome dysbiosis is a major contributing factor to several pathological conditions. However, the mechanistic understanding of the communication between gut microbiota and extra‐intestinal organs remains largely elusive. Extracellular vesicles (EVs), secreted by almost every form of life, including bacteria, could play a critical role in this inter‐kingdom crosstalk and are the focus of present study. Here, we present a novel approach for isolating lipopolysaccharide (LPS)+ bacterial extracellular vesicles (bEVLPS) from complex biological samples, including faeces, plasma and the liver from lean and diet‐induced obese (DIO) mice. bEVLPS were extensively characterised using nanoparticle tracking analyses, immunogold labelling coupled with transmission electron microscopy, flow cytometry, super‐resolution microscopy and 16S sequencing. In liver tissues, the protein expressions of TLR4 and a few macrophage‐specific biomarkers were assessed by immunohistochemistry, and the gene expressions of inflammation‐related cytokines and their receptors (n = 89 genes) were measured using a PCR array. Faecal samples from DIO mice revealed a remarkably lower concentration of total EVs but a significantly higher percentage of LPS+ EVs. Interestingly, DIO faecal bEVLPS showed a higher abundance of Proteobacteria by 16S sequencing. Importantly, in DIO mice, a higher number of total EVs and bEVLPS consistently entered the hepatic portal vein and subsequently reached the liver, associated with increased expression of TLR4, macrophage markers (F4/80, CD86 and CD206), cytokines and receptors (Il1rn, Ccr1, Cxcl10, Il2rg and Ccr2). Furthermore, a portion of bEVLPS escaped liver and entered the peripheral circulation. In conclusion, bEV could be the key mediator orchestrating various well‐established biological effects induced by gut bacteria on distant organs.

show abstract

“…Additional markers for hematopoietic stem cells could include CD117/c‐kit and lineage negative (Lin & Goodell, 2011; Mayle, Luo, Jeong, & Goodell, 2013; Rundberg Nilsson, Bryder, & Pronk, 2013). CD117 was not included due to its high expression on mast cells and endothelial cells (Foster, Zaidi, Young, Mobley, & Kerr, 2018; Foster, Langsten, Mansour, Shi, & Kerr, 2021; Li et al., 2003). For mesenchymal stem cells, we chose the markers CD29, CD90, and CD146 for primates, with the addition of Sca1 for mice.…”

Section: Commentarymentioning

confidence: 99%

A New Method of Bone Stromal Cell Characterization by Flow Cytometry

et al. 2022

Self Cite

View full text Add to dashboard Cite

The bone microenvironment cellular composition plays an essential role in bone health and is disrupted in bone pathologies, such as osteoporosis, osteoarthritis, and cancer. Flow cytometry protocols for hematopoietic stem cell lineages are well defined and well established. Additionally, a consensus for mesenchymal stem cell flow markers has been developed. However, flow cytometry markers for bone‐residing cells—osteoblasts, osteoclasts, and osteocytes—have not been proposed. Here, we describe a novel partial digestion method to separate these cells from the bone matrix and present new markers for enumerating these cells by flow cytometry. We optimized bone digestion and analyzed markers across murine, nonhuman primate, and human bone. The isolation and staining protocols can be used with either cell sorting or flow cytometry. Our method allows for the enumeration and collection of hematopoietic and mesenchymal lineage cells in the bone microenvironment combined with bone‐residing stromal cells. Thus, we have established a multi‐fluorochrome bone marrow cell‐typing methodology. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Partial digestion for murine long bone stromal cell isolation Alternate Protocol 1: Partial digestion for primate vertebrae stromal cell isolation Alternate Protocol 2: Murine vertebrae crushing for bone stromal cell isolation Basic Protocol 2: Staining of bone stromal cells Support Protocol 1: Fluorescence minus one control, isotype control, and antibody titration Basic Protocol 3: Cell sorting of bone stromal cells Alternate Protocol 3: Flow cytometry analysis of bone stromal cells Support Protocol 2: Preparing compensation beads

show abstract

Tissue distribution of stem cell factor in adults

Cited by 5 publications

References 34 publications

Loss of endothelial membrane KIT ligand affects systemic KIT ligand levels but not bone marrow hematopoietic stem cells

Loss of endothelial membrane KIT ligand affects systemic KIT ligand levels but not bone marrow hematopoietic stem cells

Characterisation of LPS+ bacterial extracellular vesicles along the gut‐hepatic portal vein‐liver axis

A New Method of Bone Stromal Cell Characterization by Flow Cytometry

Contact Info

Product

Resources

About