Tissue distribution of sulfolipids in the rat. Restricted location of sulfatoxygalactosylacylalkylglyerol

Lingwood, Clifford A.; Hay, Genevieve H.; Schachter, Harry

doi:10.1139/o81-077

Cited by 28 publications

(10 citation statements)

References 4 publications

(4 reference statements)

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“…In addition, SGC is the major glycolipid of the human endometrium which varies during the menstrual cycle (Kubushiro et al, 1989). SGC is also a significant fraction of the glycolipid of the kidney, brain, and gastrointestinal tract (Lingwood et al, 1981;Murray et al, 1980). We have previously isolated a plasma membrane sulfoglycolipid binding protein, termed SLIP 1, from rat testicular germ cells by SGG affinity chromatography (Lingwood, 1985).…”

mentioning

confidence: 99%

Members of the 70 kDa heat shock protein family specifically recognize sulfoglycolipids: Role in gamete recognition and mycoplasma‐related infertility

Boulanger

Faulds²,

Eddy

et al. 1995

Journal Cellular Physiology

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We have previously shown that several mycoplasma species associated with infertility bind specifically to sulfated glycolipids isolated from the mammalian reproductive tract. We now show that a germ cell-specific sulfoglycolipid binding protein (SLIP 1), which is a potent inhibitor of sperm/egg binding in vitro, is immunologically related to the heat shock protein(Hsp) 70 family of stress proteins and that Hsps are surface antigens in male germ cells. Our present data demonstrate that several mycoplasma and mammalian Hsps share this glycolipid binding specificity in vitro, and suggest that surface Hsps can function as adhesins which mediate sulfoglycolipid recognition in infectious disease and normal reproductive physiology.

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confidence: 99%

Members of the 70 kDa heat shock protein family specifically recognize sulfoglycolipids: Role in gamete recognition and mycoplasma‐related infertility

Boulanger

Faulds²,

Eddy

et al. 1995

Journal Cellular Physiology

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“…In the ileal sections, SGC is expressed in the villus crypts from normal mice. SGC is a major GSL in the mouse kidney (Lingwood et al, 1981), but ΔF508CFTR-transgenic mouse kidney showed no SGC. These results showed that there are dramatic changes of SGC expression in CFTR defective mice.…”

Section: Resultsmentioning

confidence: 99%

A Soluble Sulfogalactosyl Ceramide Mimic Promotes ΔF508 CFTR Escape from Endoplasmic Reticulum Associated Degradation

Park

Mylvaganum

McPherson

et al. 2009

Chemistry & Biology

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SUMMARY AdaSGC binds Hsc70s to inhibit ATPase activity. Using single-turnover assays, adaSGC, a soluble SGC mimic, preferentially inhibited Hsp40-activated Hsc70 ATP hydrolysis (Ki ~ 10 μM) to reduce C-terminal Hsc70-peptide binding and, potentially, chaperone function. ERAD of misfolded ΔF508 CFTR requires Hsc70-Hsp40 chaperones. In transfected baby hamster kidney (BHK) cells, adaSGC increased ΔF508CFTR ERAD escape, and after low-temperature glycerol rescue, maturation, and iodide efflux. Inhibition of SGC biosynthesis reduced ΔF508CFTR but not wtCFTR expression, whereas depletion of other glycosphingolipids had no affect. WtCFTR transfected BHK cells showed increased SGC synthesis compared with ΔF508CFTR/mock-transfected cells. Partial rescue of ΔF508CFTR by low-temperature glycerol increased SGC synthesis. AdaSGC also increased cellular endogenous SGC levels. SGC in the lung, liver, and kidney was severely depleted in ΔF508CFTR compared with wtCFTR mice, suggesting a role for CFTR in SGC biosynthesis.

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“…"SGG was prepared in vitro from PAP35S by use of the renal galactolipid sulfotransferase (Taylor et al, 1987) since this tissue contains no endogenous SGG (Lingwood et al, 1981), yet GG remains a n effective substrate in vitro (Lingwood, 1985~). "SGC was prepared by using the testicular enzyme for a similar reason.…”

Section: Discussionmentioning

confidence: 99%

“…GG was prepared from SGG by anchimeric assisted solvolysis (Lingwood et al, 1983). SGG was deacylated by mild basic hydrolysis (Lingwood et al, 1981) GC and SGC were purchased from Sigma (St. Louis, MO). GM, and GM, gangliosides were kindly provided by Dr. A. Novak, Department of Neurosciences, Hospital for Sick Children.…”

Section: Methodsmentioning

confidence: 99%

Male germ cell specific sulfogalactoglycerolipid is recognized and degraded by mycoplasmas associated with male infertility

Lingwood

Schramayr

Quinn

1990

Journal Cellular Physiology

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Sulfogalactosylglycerolipid (SGG) is the major mammalian male germ cell glycolipid and has been implicated in sperm/egg binding. Mycoplasma pulmonis, a species of Mollicutes, is associated with male infertility in rodents. Purified SGG incubated in the presence of M. pulmonis was enzymatically degraded by both desulfation and deacylation. Desulfation occurred primarily at alkaline pH, and deacylation also increased with increased pH, indicating that these represent novel enzymatic activities. Digestion was facilitated, but not dependent on, the presence of detergent. Rat spermatozoa exposed to M. pulmonis showed a reduction in SGG content which was particularly marked for cauda (mature) spermatozoa. With the aid of tlc overlay binding procedure, intact M. pulmonis were found to bind specifically to sulfated glycolipids and thus SGG may provide the cell membrane receptor for this organism. The topology of mycoplasma binding to rat sperm was consistent with the known topology of sperm SGG. The reduced binding (and subsequent digestion) of caput spermatozoan SGG correlates with the membrane colocalization of SGG and its endogenous binding protein at this stage. Separation of SGG and its binding protein during epididymal sperm maturation appears to facilitate M. pulmonis binding to and digestion of cauda sperm SGG. The binding and degradation of the sperm SGG by M. pulmonis may play a role in the induction of infertility which follows infection with these organisms by interfering in sperm/egg receptor recognition.

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Tissue distribution of sulfolipids in the rat. Restricted location of sulfatoxygalactosylacylalkylglyerol

Cited by 28 publications

References 4 publications

Members of the 70 kDa heat shock protein family specifically recognize sulfoglycolipids: Role in gamete recognition and mycoplasma‐related infertility

Members of the 70 kDa heat shock protein family specifically recognize sulfoglycolipids: Role in gamete recognition and mycoplasma‐related infertility

A Soluble Sulfogalactosyl Ceramide Mimic Promotes ΔF508 CFTR Escape from Endoplasmic Reticulum Associated Degradation

Male germ cell specific sulfogalactoglycerolipid is recognized and degraded by mycoplasmas associated with male infertility

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