Tissue Electropotentials in Kalanchoë Blossfeldiana During Wound Healing

Abstract

Penetrating palladium probes were placed in the stem and leaf of Kalancho'ë blossfeldiana. The electropotentials arising in the probes at the wound site were continuously monitored during the subsequent healing process. The potential change was always in the positive going direction, beginning at an average of 320 millivolts and stabilizing at an average of 601 millivolts relative to a standard hydrogen electrode. The healing process can be divided into two distinct phases: an initial phase of approximately 6 hr duration in which the potential rises rapidly and a second phase extending to approximately dusk on the third day in which the potential rises at a substantially lower rate. There is also a definite change of the transition potential waveshape at the beginning and end of the 16‐hr light period during the healing process. Rewounding results in a potential recovery waveshape similar to the original healing response but of shorter duration. The data are interpreted in terms of the redox status of the wound site.

429 — A physiological basis for electrophytograms

“…It is necessary to understand exactly what is occurring at the point of contact between the metal and the plant. Gensler (1978b) has pointed out the paucity of literature on the subject of plant wound healing around a penetrating, chemically inactive object. It might be expected that, if an object is truly inert, normal repair processes would occur.…”

An Electrochemical Instrumentation System for Agriculture and the Plant Sciences

“…Healing potentials are observed immediately after the penetration of the probe into the tissue (4,5). The potential drops precipitously.…”

“…The selenide solutions used in these cells are colored, air sensitive, and highly toxic, properties which would make a suitable substitute attractive. Bard (3) and others (4,5) are attempting to use nonaqueous solvents to avoid the photocorrosion reactions which involve water and to make available a wider range of organic and organometallic redox couples for use in these cells, however the limited solubility of many of these couples has precluded their use at light intensities near or above solar levels (3). The difficulty of permanently removing water from the organic solvents and the additional expense they would add to cell construction are other problems associated with the use of these solvents.…”

Investigation of the Causative Reactant of the Apoplast Electropotentials of Plants

“…A rest potential is measured across the two electrodes which has been found to be stable and reproducible. Furthermore, the potential elicits definite potential-time patterns 2 termed phytograms which relate to the plant condition and changes in the soil or atmospheric environment (1)(2)(3)(4)(5)(6)(7)(8)(9)(10).…”