Tissue-engineered alloplastic scaffolds for reconstruction of alveolar defects

Witek, Lukasz; Colon, Ricardo Rodriguez; Wang, Maxime M.; Torroni, Andrea; Young, Simon; Melville, James C.; Lopez, Christopher D.; Flores, Roberto L.; Cronstein, Bruce N.; Coelho, Paulo G.

doi:10.1016/b978-0-08-102563-5.00024-1

Cited by 10 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3D-printing technology has been playing a pivotal role in regenerative medicine. It has paved the way to customized soft and hard tissue reconstruction and rehabilitation by presenting opportunities to fabricate patient-specific, space-maintaining scaffolds tailored to site-specific defects that are osteogenic, osteoconductive, osteoinductive, facilitate angiogenesis/vasculogenesis, and are mechanically stable upon implantation to prevent immediate failure 14,22,28,81,82 …”

Section: Evolution and Optimization Of Scaffolds For Craniofacial Bon...mentioning

confidence: 99%

“…Current available modalities assisting in bone regeneration and repair of defects include the autogenous, allogenous, xenogenous, or alloplastic particulate bone grafts in addition to synthetic scaffolds 4,10–24 . All have various degrees of osteogenic, osteoinductive and osteoconductive potential.…”

mentioning

confidence: 99%

“…Xenografts, which are commonly bovine or porcine-derived, are processed to produce a deproteinized, porous hydroxyapatite (HA) scaffold with high osteoconductivity and lower immunogenicity relative to the allograft 29 . Currently, a great deal of scientific innovation has led to advances in craniomaxillofacial tissue engineering (TE), and applications of TE to reconstructive surgery, with the objective of decreasing the invasiveness of the procedures and eliminating potential biological complications witnessed with allografts or xenografts 17–24 …”

mentioning

confidence: 99%

“…These alloplastic provide a readily available, wide array of reconstructive options. However, alloplastic materials are limited by their decreased osteogenic and angiogenic potential, high costs, unfavorable immune response, and high infection and extrusion rates, thereby necessitating further refinement 17–24 …”

mentioning

confidence: 99%

See 3 more Smart Citations

Bone Tissue Engineering (BTE) of the Craniofacial Skeleton, Part I: Evolution and Optimization of 3D-Printed Scaffolds for Repair of Defects

Nayak,

Slavin,

Bergamo

et al. 2023

Journal of Craniofacial Surgery

View full text Add to dashboard Cite

Bone tissue regeneration is a complex process that proceeds along the well-established wound healing pathway of hemostasis, inflammation, proliferation, and remodeling. Recently, tissue engineering efforts have focused on the application of biological and technological principles for the development of soft and hard tissue substitutes. Aim is directed towards boosting pathways of the healing process to restore form and function of tissue deficits. Continued development of synthetic scaffolds, cell therapies, and signaling biomolecules seeks to minimize the need for autografting. Despite being the current gold standard treatment, it is limited by donor sites’ size and shape, as well as donor site morbidity. Since the advent of computer-aided design/computer-aided manufacturing (CAD/CAM) and additive manufacturing (AM) techniques (3D printing), bioengineering has expanded markedly while continuing to present innovative approaches to oral and craniofacial skeletal reconstruction. Prime examples include customizable, high-strength, load bearing, bioactive ceramic scaffolds. Porous macro- and micro-architecture along with the surface topography of 3D printed scaffolds favors osteoconduction and vascular in-growth, as well as the incorporation of stem and/or other osteoprogenitor cells and growth factors. This includes platelet concentrates (PCs), bone morphogenetic proteins (BMPs), and some pharmacological agents, such as dipyridamole (DIPY), an adenosine A2A receptor indirect agonist that enhances osteogenic and osteoinductive capacity, thus improving bone formation. This two-part review commences by presenting current biological and engineering principles of bone regeneration utilized to produce 3D-printed ceramic scaffolds with the goal to create a viable alternative to autografts for craniofacial skeleton reconstruction. Part II comprehensively examines recent preclinical data to elucidate the potential clinical translation of such 3D-printed ceramic scaffolds.

show abstract

Section: Evolution and Optimization Of Scaffolds For Craniofacial Bon...mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Bone Tissue Engineering (BTE) of the Craniofacial Skeleton, Part I: Evolution and Optimization of 3D-Printed Scaffolds for Repair of Defects

Nayak,

Slavin,

Bergamo

et al. 2023

Journal of Craniofacial Surgery

View full text Add to dashboard Cite

show abstract

“…13,14 Furthermore, these 3D printed scaffolds can be customized to fill any defect site precisely. 14,15 The osteogenic potential of these bone tissue engineering scaffolds can be further augmented by osteogenic agents such as recombinant human bone morphogenic protein-2 (rhBMP-2) and dipyridamole. 14,[16][17][18] Previous studies, using several different animal models, have demonstrated the ability of 3D-printed bioceramic (3DPBC) scaffolds composed of 100% β-TCP to regenerate bone within critical-sized defects with bone morphology and mechanical properties that are equivalent to native bone.…”

mentioning

confidence: 99%

“Bone Tissue Engineering in the Growing Calvaria: A 3D Printed Bioceramic Scaffold to Reconstruct Critical-Sized Defects in a Skeletally Immature Pig Model”

DeMitchell-Rodriguez

Shen

Nayak

et al. 2023

Plastic &Amp; Reconstructive Surgery

Self Cite

View full text Add to dashboard Cite

Background: Three-dimensional printed bioceramic scaffolds composed of 100% β-tricalcium phosphate augmented with dipyridamole (3DPBC-DIPY) can regenerate bone across critically sized defects in skeletally mature and immature animal models. Before human application, safe and effective bone formation should be demonstrated in a large translational animal model. This study evaluated the ability of 3DPBC-DIPY scaffolds to restore critically sized calvarial defects in a skeletally immature, growing minipig. Methods: Unilateral calvarial defects (~1.4 cm) were created in 6-week-old Göttingen minipigs (n = 12). Four defects were filled with a 1000 μm 3DPBC-DIPY scaffold with a cap (a solid barrier on the ectocortical side of the scaffold to prevent soft-tissue infiltration), four defects were filled with a 1000 μm 3DPBC-DIPY scaffold without a cap, and four defects served as negative controls (no scaffold). Animals were euthanized 12 weeks postoperatively. Calvariae were subjected to micro–computed tomography, 3D reconstruction with volumetric analysis, qualitative histologic analysis, and nanoindentation. Results: Scaffold-induced bone growth was statistically greater than in negative controls (P ≤ 0.001), and the scaffolds with caps produced significantly more bone generation compared with the scaffolds without caps (P ≤ 0.001). Histologic analysis revealed woven and lamellar bone with haversian canals throughout the regenerated bone. Cranial sutures were observed to be patent, and there was no evidence of ectopic bone formation or excess inflammatory response. Reduced elastic modulus and hardness of scaffold-regenerated bone were found to be statistically equivalent to native bone (P = 0.148 for reduced elastic modulus of scaffolds with and without caps and P = 0.228 and P = 0.902 for hardness of scaffolds with and without caps, respectively). Conclusion: 3DPBC-DIPY scaffolds have the capacity to regenerate bone across critically sized calvarial defects in a skeletally immature translational pig model. Clinical Relevance Statement: This study assessed the bone generative capacity of 3D-printed bioceramic scaffolds composed of 100% β-tricalcium phosphate and augmented with dipyridamole placed within critical-sized calvarial defects in a growing porcine model.

show abstract