Tissue engineered bone-regeneration using degradable polymers: The formation of mineralized matrices

Laurencin, Cato T.; Attawia, Mohamed; Elgendy, Hoda M.; Herbert, Kelly M.

doi:10.1016/s8756-3282(96)00132-9

Cited by 157 publications

(82 citation statements)

References 16 publications

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“…Bone tissue formation throughout the scaffold has been demonstrated [86]. Several other groups fabricated PLGA/ HAP or PLGA/PCL/HAP composite scaffolds using a salt-leaching technique [104][105][106][107]. Although the salt-leaching technique has limitations in generating highly porous and wellconnected pore structures, they also consistently demonstrated the improved osteoconductivity over the PLGA scaffolds.…”

Section: Composite and Nano-composite Materialsmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,213

819

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Section: Composite and Nano-composite Materialsmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,213

819

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“…Osteogenic growth factors typically include ascorbic acid to aid collagen synthesis and some form of phosphate donor which has been shown to be beneficial for in vitro mineralisation (Laurencin et al, 1996). To this, vitamins D 3 and K 3 and recombinant human TGF-β 1 , key regulators of bone formation, were added as this combination 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 F o r P e e r R e v i e w gave optimal results in our 2D culture studies (data not shown).…”

Section: Introductionmentioning

confidence: 99%

A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering

2010

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• to copy, distribute, display, and perform the work.• to make derivative works. Under the following conditions:• Attribution -You must give the original author credit.• Non-Commercial -You may not use this work for commercial purposes.• suggest the benefit of introducing an initial high treatment of TGF-β 1 (10ng/ml) followed by a low continuous treatment (0.2ng/ml) to enhance human osteogenesis on the scaffold. Osteogenesis coincided with a reduction in scaffold size and shape (up to 70% that of original). A notable finding was that after 49 days of culture, core degradation was identified at the centre of the tissue engineered construct. This was not observed at earlier time points therefore a maximum of 35 days in culture to be an appropriate end point for in vitro studies of these scaffolds. Weconclude that the CG scaffold shows excellent potential as a biomaterial for human bone tissue engineering.

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“…Composite scaffolds may prove necessary for reconstruction of multitissue organs, tissues interfaces, and structural tissue including bone, cartilage, tendons, ligaments and muscles. Ceramics including dense and porous hydro-xyapatite (HA), tricalcium phosphate (TCP) ceramics and bioactive glasses and glass-ceramics have been combined with a large number of polymers including natural collagen [4], chitosan [5], non-biodegradable poly(ethylene) [6], poly(methyl methacrylate) [7,8], polylsulfone [9], and biodegradable poly(α-hydroxya-cids) [10][11][12][13][14][15][16]. Bioresorbable poly(lactic acid) (PLA), poly(glycolic acid) (PGA), and poly(lactic acid-co-glycolic acid) (PLGA) copolymers are very attractive for scaffolds for tissue engineering [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Melt-extrusion or compression-molding of PLA and bioactive glass or HA particles was used for the preparation of non-porous bone fixation devices [15]. Compatible polymer processing techniques including combined solvent-casting and salt-leaching [14,19], phase separation and freeze-drying [13] and immersion-precipitation [20] have been used for the preparation of highly porous PLLA/ HA and PLGA/HA scaffolds. Each processing method has advantages that suit different tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation

et al. 2004

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Highly porous composites scaffolds of poly-D,L-lactide (PDLLA) and poly(lactide-co-glycolide) (PLGA) containing different amounts (10, 25 and 50wt%) of bioactive glass (45S5 bioglass ® ) were prepared by thermally induced solid-liquid phase separation (TIPS) and subsequent solvent sublimation. The addition of increasing amounts of bioglass ® into the polymer foams decreased the pore volume. Conversely, the mechanical properties of the polymer materials were improved. The composites were incubated in phosphate buffer saline at 37°C to study the in vitro degradation of the polymer by measurement of water absorption, weight loss as well as changes in the average molecular weight of the polymer and in the pH of the incubation medium as a function of the incubation time. The addition of bioglass ® to polymer foams increased the water absorption and weight loss compared to neat polymer foams. However, the polymer molecular weight, determined by size exclusion chromatography, was found to decrease more rapidly and to a larger extent in absence of bioglass ® . The presence of the bioactive filler was therefore found to delay the degradation rate of the polymer as compared to the neat polymer foams. Formation of hydroxyapatite on the surface of composites, as an indication of their bioactivity, was recorded by EDXA, X-ray diffractometry and confirmed by Raman spectroscopy.

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Tissue engineered bone-regeneration using degradable polymers: The formation of mineralized matrices

Cited by 157 publications

References 16 publications

Biomimetic materials for tissue engineering

Biomimetic materials for tissue engineering

A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering

Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation

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