Tissue-engineered peripheral nerve grafting by differentiated bone marrow stromal cells

Hou, Sheng‐Mou; Zhang, H.-Y.; Quan, Daping; Liu, X.-L.; Jing, Zhu

doi:10.1016/j.neuroscience.2006.01.066

Cited by 78 publications

(56 citation statements)

References 24 publications

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“…Commercially available natural conduits are usually composed of ECM components such as collagen [38] and fibrin [28] . Synthetic materials include polyglycolic acid [57] , Poly(lactic-coglycolic) acid [164] , silicone tube [39,43] , polyhydroxybutyrate [20] , polytetrafluoroethylene [24] silk fibroin [42] and chitosan [91,165] . The degradation profile of conduits must be carefully considered when used with cell therapy.…”

Section: Cell Scaffolds and Methods Of Deliverymentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

128

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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Section: Cell Scaffolds and Methods Of Deliverymentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

128

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show abstract

“…Polylactic-glycolic acid (PLGA) is one of the top biodegradable synthetic polymers used for tissue engineering due to the ease of controlling its mechanical properties and biodegradation rate [6]. PLGA hollow tubes filled with cells have been studied for use in nerve graft and showed promising results [7,8]. However, the hydrophobicity of PLGA and absence of cell recognition sites on the PLGA scaffold may hinder the cell adhesion onto PLGA surface [9].…”

mentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits (Adv Clin Exp Med 2014, 23, 3, 353-362).

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“…As demonstrated previously, BMSCs implanted within the conduits can synthesize neurotrophic factors and extracellular matrix elements for peripheral nerve regeneration (2,25). Furthermore it was well documented that BMSCs can differentiate exclusively into SCs in vivo (3,6,25) and in vitro (7,11,12,16,18,23) and promote axonal regeneration if the appropriate microenvironment or cell culture conditions were constituted.…”

mentioning

confidence: 67%

“…BMSCs have pluripotent plasticity to differentiate into different cells including neural cell lineage under convenient conditions (9). Recently, introduction of undifferentiated (2,6,12,20) and transdifferentiated (7,11,(16)(17)(18) BMSCs into nerve conduits represents a popular approach for peripheral nerve regeneration. It has been demonstrated that BMSCs in the surgical repair of peripheral nerves have improved axonal regeneration and functional recovery.…”

Section: Histological and Immunohistological Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Nerve tÝssue prefabrÝcatÝon ÝnsÝde the rat femoral bone: does Ýt work?

Özbek

Koçman²,

Özatik³

et al. 2015

Turkish Neurosurgery

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Tissue-engineered peripheral nerve grafting by differentiated bone marrow stromal cells

Cited by 78 publications

References 24 publications

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit

Nerve tÝssue prefabrÝcatÝon ÝnsÝde the rat femoral bone: does Ýt work?

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